Transcript: Human NM_006310.4

Homo sapiens aminopeptidase puromycin sensitive (NPEPPS), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
NPEPPS (9520)
Length:
4309
CDS:
194..2953

Additional Resources:

NCBI RefSeq record:
NM_006310.4
NBCI Gene record:
NPEPPS (9520)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006310.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000313550 AGAAGCCCGTCGTCGGTTTAA pLKO_005 2362 CDS 100% 13.200 18.480 N Npepps n/a
2 TRCN0000073842 GCAGGACATAAGGCAACGTTA pLKO.1 2339 CDS 100% 4.950 6.930 N NPEPPS n/a
3 TRCN0000291128 GCAGGACATAAGGCAACGTTA pLKO_005 2339 CDS 100% 4.950 6.930 N NPEPPS n/a
4 TRCN0000331163 TACCGAACAGCTGATTCATAT pLKO_005 3011 3UTR 100% 0.000 0.000 N NPEPPS n/a
5 TRCN0000296878 TTGTGAATGAGCCCAATTATA pLKO_005 2121 CDS 100% 15.000 10.500 N NPEPPS n/a
6 TRCN0000313586 TTGTGAATGAGCCCAATTATA pLKO_005 2121 CDS 100% 15.000 10.500 N Npepps n/a
7 TRCN0000073841 CAATGGGTGAAGTTAAACTTA pLKO.1 1922 CDS 100% 5.625 3.938 N NPEPPS n/a
8 TRCN0000073838 CCAAGGATTGTAGTTTAGTTT pLKO.1 3521 3UTR 100% 5.625 3.938 N NPEPPS n/a
9 TRCN0000073840 CCAGACCAATGGGTGAAGTTA pLKO.1 1916 CDS 100% 5.625 3.938 N NPEPPS n/a
10 TRCN0000291188 CCAGACCAATGGGTGAAGTTA pLKO_005 1916 CDS 100% 5.625 3.938 N NPEPPS n/a
11 TRCN0000073839 GCTGGAATCATTAGCACTGTA pLKO.1 2075 CDS 100% 4.950 3.465 N NPEPPS n/a
12 TRCN0000291187 GCTGGAATCATTAGCACTGTA pLKO_005 2075 CDS 100% 4.950 3.465 N NPEPPS n/a
13 TRCN0000052407 CGGGAACCTTAAAGATAGATT pLKO.1 615 CDS 100% 5.625 3.375 N NPEPPSP1 n/a
14 TRCN0000313596 TGATGAATTGTGCTGATATTG pLKO_005 474 CDS 100% 13.200 6.600 Y Npepps n/a
15 TRCN0000031106 GCCTGCTATCAAAGCAACTTT pLKO.1 772 CDS 100% 5.625 2.813 Y Npepps n/a
16 TRCN0000317190 GCCTGCTATCAAAGCAACTTT pLKO_005 772 CDS 100% 5.625 2.813 Y Npepps n/a
17 TRCN0000031105 GCCTTGTTACTTATAGGGAAA pLKO.1 1158 CDS 100% 4.050 2.430 N Npepps n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006310.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11380 pDONR223 100% 95.2% 95.2% None 1_132del n/a
2 ccsbBroad304_11380 pLX_304 0% 95.2% 95.2% V5 1_132del n/a
3 TRCN0000476461 ATGTGTCTCAGCCTTCGAGTTGCC pLX_317 14.1% 95.2% 95.2% V5 1_132del n/a
Download CSV