Transcript: Human XM_017025373.1

PREDICTED: Homo sapiens aminopeptidase puromycin sensitive (NPEPPS), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NPEPPS (9520)
Length:
4236
CDS:
38..2800

Additional Resources:

NCBI RefSeq record:
XM_017025373.1
NBCI Gene record:
NPEPPS (9520)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017025373.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000313550 AGAAGCCCGTCGTCGGTTTAA pLKO_005 2194 CDS 100% 13.200 18.480 N Npepps n/a
2 TRCN0000073842 GCAGGACATAAGGCAACGTTA pLKO.1 2171 CDS 100% 4.950 6.930 N NPEPPS n/a
3 TRCN0000291128 GCAGGACATAAGGCAACGTTA pLKO_005 2171 CDS 100% 4.950 6.930 N NPEPPS n/a
4 TRCN0000331163 TACCGAACAGCTGATTCATAT pLKO_005 2938 3UTR 100% 0.000 0.000 N NPEPPS n/a
5 TRCN0000296878 TTGTGAATGAGCCCAATTATA pLKO_005 1953 CDS 100% 15.000 10.500 N NPEPPS n/a
6 TRCN0000313586 TTGTGAATGAGCCCAATTATA pLKO_005 1953 CDS 100% 15.000 10.500 N Npepps n/a
7 TRCN0000073841 CAATGGGTGAAGTTAAACTTA pLKO.1 1754 CDS 100% 5.625 3.938 N NPEPPS n/a
8 TRCN0000073838 CCAAGGATTGTAGTTTAGTTT pLKO.1 3448 3UTR 100% 5.625 3.938 N NPEPPS n/a
9 TRCN0000073840 CCAGACCAATGGGTGAAGTTA pLKO.1 1748 CDS 100% 5.625 3.938 N NPEPPS n/a
10 TRCN0000291188 CCAGACCAATGGGTGAAGTTA pLKO_005 1748 CDS 100% 5.625 3.938 N NPEPPS n/a
11 TRCN0000073839 GCTGGAATCATTAGCACTGTA pLKO.1 1907 CDS 100% 4.950 3.465 N NPEPPS n/a
12 TRCN0000291187 GCTGGAATCATTAGCACTGTA pLKO_005 1907 CDS 100% 4.950 3.465 N NPEPPS n/a
13 TRCN0000052407 CGGGAACCTTAAAGATAGATT pLKO.1 447 CDS 100% 5.625 3.375 N NPEPPSP1 n/a
14 TRCN0000313596 TGATGAATTGTGCTGATATTG pLKO_005 306 CDS 100% 13.200 6.600 Y Npepps n/a
15 TRCN0000031106 GCCTGCTATCAAAGCAACTTT pLKO.1 604 CDS 100% 5.625 2.813 Y Npepps n/a
16 TRCN0000317190 GCCTGCTATCAAAGCAACTTT pLKO_005 604 CDS 100% 5.625 2.813 Y Npepps n/a
17 TRCN0000031105 GCCTTGTTACTTATAGGGAAA pLKO.1 990 CDS 100% 4.050 2.430 N Npepps n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017025373.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11380 pDONR223 100% 89.6% 88.7% None 1_120del;2547_2641del;2760_2761ins80 n/a
2 ccsbBroad304_11380 pLX_304 0% 89.6% 88.7% V5 1_120del;2547_2641del;2760_2761ins80 n/a
3 TRCN0000476461 ATGTGTCTCAGCCTTCGAGTTGCC pLX_317 14.1% 89.6% 88.7% V5 1_120del;2547_2641del;2760_2761ins80 n/a
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