Construct: ORF ccsbBroad304_11418

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000285.1_s304c1, BRDN0000400286
Derived from:
ccsbBroadEn_11418
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GIT2 (9815)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11418
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9815 GIT2 GIT ArfGAP 2 NM_057170.5 86.3% 86.3% 1_258del
2 human 9815 GIT2 GIT ArfGAP 2 XM_006719712.4 80.8% 80.8% 1_258del;1238_1239ins105
3 human 9815 GIT2 GIT ArfGAP 2 NM_014776.5 78.3% 76.1% (many diffs)
4 human 9815 GIT2 GIT ArfGAP 2 NM_001135213.3 78% 75.7% (many diffs)
5 human 9815 GIT2 GIT ArfGAP 2 XM_006719708.4 76.3% 76.3% 1_258del;1348_1596del
6 human 9815 GIT2 GIT ArfGAP 2 XM_017020258.2 76.1% 76.1% 1_258del;761_762insCAG;1345_1593del
7 human 9815 GIT2 GIT ArfGAP 2 XM_006719709.4 75.3% 73.6% (many diffs)
8 human 9815 GIT2 GIT ArfGAP 2 NM_001330153.2 75.2% 73.4% (many diffs)
9 human 9815 GIT2 GIT ArfGAP 2 NM_001135214.3 74.7% 74.7% 1_258del;1348_1641del
10 human 9815 GIT2 GIT ArfGAP 2 XM_006719707.4 73.2% 73.2% 1_258del;1347_1685del
11 human 9815 GIT2 GIT ArfGAP 2 NM_057169.5 71.8% 71.8% 1_258del;1347_1730del
12 human 9815 GIT2 GIT ArfGAP 2 XM_005253997.4 71.6% 71.6% 1_258del;761_762insCAG;1344_1727del
13 human 9815 GIT2 GIT ArfGAP 2 XM_017020260.2 65.5% 65.4% (many diffs)
14 human 9815 GIT2 GIT ArfGAP 2 XM_017020259.2 62.9% 62.8% (many diffs)
15 human 9815 GIT2 GIT ArfGAP 2 NM_139201.3 60% 56.9% (many diffs)
16 human 9815 GIT2 GIT ArfGAP 2 XM_017020261.2 59.8% 56.8% (many diffs)
17 human 9815 GIT2 GIT ArfGAP 2 XM_006719713.4 58% 57.8% (many diffs)
18 human 9815 GIT2 GIT ArfGAP 2 NM_001330154.2 52.9% 52.2% (many diffs)
19 mouse 26431 Git2 G protein-coupled receptor ... NM_001077360.1 69.1% 70.9% (many diffs)
20 mouse 26431 Git2 G protein-coupled receptor ... NM_001077359.1 68.9% 70.5% (many diffs)
21 mouse 26431 Git2 G protein-coupled receptor ... XM_006530338.3 68.9% 70.6% (many diffs)
22 mouse 26431 Git2 G protein-coupled receptor ... XM_017320893.1 66.3% 68.1% (many diffs)
23 mouse 26431 Git2 G protein-coupled receptor ... NM_019834.3 66.1% 67.9% (many diffs)
24 mouse 26431 Git2 G protein-coupled receptor ... XM_006530337.3 66.1% 67.7% (many diffs)
25 mouse 26431 Git2 G protein-coupled receptor ... XM_017320892.1 66.1% 67.7% (many diffs)
26 mouse 26431 Git2 G protein-coupled receptor ... XM_006530336.3 65.5% 69.5% (many diffs)
27 mouse 26431 Git2 G protein-coupled receptor ... XM_006530335.3 65.3% 69.2% (many diffs)
28 mouse 26431 Git2 G protein-coupled receptor ... XM_006530334.3 64% 67.8% (many diffs)
29 mouse 26431 Git2 G protein-coupled receptor ... NM_001347400.1 62.9% 66.7% (many diffs)
30 mouse 26431 Git2 G protein-coupled receptor ... XM_006530333.3 62.8% 66.6% (many diffs)
31 mouse 26431 Git2 G protein-coupled receptor ... XM_006530339.3 62.8% 66.4% (many diffs)
32 mouse 26431 Git2 G protein-coupled receptor ... XM_006530331.3 62.8% 66.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1701
ORF length:
1635
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag tggaagacgt aaagctaatc cacaggataa agtacatccc aataaagcgg
121aattcatcag agccaagtat cagatgttag cgttcgtcca tcgcttgccc tgccgggatg
181acgatagtgt gactgccaaa gatcttagca agcaactcca ttcgagcgtg agaacaggga
241atcttgaaac ctgtttgaga ctgttatctt taggagcaca agccaacttc tttcatcctg
301aaaaaggaaa caccccactc catgttgcct ccaaagcagg gcagatttta caggctgaat
361tattggcagt atatggagca gacccaggca cacaggattc tagtgggaaa actcccgttg
421attatgcaag gcaaggaggg caccatgagc tggcagagcg cctcgtggaa atacagtatg
481agctaacgga cagactagcc ttctatctct gtggcaggaa accagatcac aaaaatggac
541agcactttat aatacctcaa atggcagaca gcagcctgga tttgtctgaa ttggcaaaag
601ctgctaagaa gaaacttcaa tctctaagta atcatttgtt tgaagaactt gccatggatg
661tgtacgatga agttgacagg cgagagacgg atgcagtctg gcttgccacg caaaaccaca
721gcgccctggt aaccgagaca acggtcgtcc cctttcttcc ggtcaatcct gagtactcat
781caacacgaaa tcagggcaga cagaagttag ctcggttcaa cgcccatgag tttgccacgc
841tggtcattga cattctcagt gacgccaaga ggagacagca gggcagttct ctctcgggtt
901caaaagacaa tgtggagctc atactgaaaa ccatcaataa ccagcacagc gttgagagtc
961aagacaacga tcagcccgac tatgacagcg tggcatcaga cgaagacaca gatttggaaa
1021ccactgcaag caaaacaaac cggcagaaga gcctagattc agatttatca gatggaccag
1081tcactgtaca ggaatttatg gaggtcaaaa acgctctagt ggcttctgag gccaagatac
1141agcagctaat gaaggcatcc aggctggaga agcagaacag cacacctgag agtgactacg
1201acaacactcc caacgacatg gagccagatg gcatggggtc aagccgaaag ggacggcaaa
1261gaagtatggt gtggccaggg gatggcttgg taccagacac agcagaaccc catgtggccc
1321caagccccac tctccctagc accgaagatg tcatcaggaa gactgaacag atcaccaaaa
1381acatacagga gctcttaaga gcagcccaag aaaataaaca tgacagttat attccctgct
1441cagagaggat acacgtagct gttacagaaa tggcagcatt attccccaaa aaacccaagt
1501ctgatatggt gaggacttcc cttcgtttac tgacgtccag tgcctaccga ctgcagtcag
1561agtgcaagaa gaccctccca ggggaccccg gctcacccac agacgttcag ctggtcacgc
1621agcaggtcat ccagtgtgcg tacgacatcg ccaaggctgc caagcagctg gttaccatca
1681ccaccaaaga gaacaacaac tgcccaactt tcttgtacaa agtggttggt aagcctatcc
1741ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1801ttaagtcgac aatcaacctc tggatta

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