Construct: ORF ccsbBroad304_11438

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001869.1_s304c1, BRDN0000402310
Derived from:
ccsbBroadEn_11438
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC23A1 (9963)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11438
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9963 SLC23A1 solute carrier family 23 me... XM_011543769.1 61.8% 51.5% (many diffs)
2 human 9963 SLC23A1 solute carrier family 23 me... XM_011543767.1 48.5% 48.5% 1_108del;378_1091del
3 human 9963 SLC23A1 solute carrier family 23 me... XM_011543768.1 45.8% 38.3% (many diffs)
4 human 9963 SLC23A1 solute carrier family 23 me... XM_011543766.1 45.8% 45.8% 1_108del;378_1187del
5 human 9963 SLC23A1 solute carrier family 23 me... NM_005847.5 43.3% 43.3% 270_1286del
6 human 9963 SLC23A1 solute carrier family 23 me... NM_152685.3 43% 43% 270_1298del
7 human 9963 SLC23A1 solute carrier family 23 me... XM_005272149.4 40.8% 40.8% 1_108del;378_1394del
8 human 9963 SLC23A1 solute carrier family 23 me... XM_005272148.3 40.5% 40.5% 1_108del;378_1406del
9 human 9963 SLC23A1 solute carrier family 23 me... XM_011543765.2 40.5% 40.5% 1_108del;378_1406del
10 human 9963 SLC23A1 solute carrier family 23 me... XM_006714741.2 35.5% 35.5% 1_108del;378_1406del;1570_1571ins96
11 mouse 20522 Slc23a1 solute carrier family 23 (n... NM_011397.4 36.9% 36.6% (many diffs)
12 mouse 20522 Slc23a1 solute carrier family 23 (n... XM_017317851.1 36.9% 36.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
843
ORF length:
777
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag ggcccaggag gacctcgagg gccggacaca gcatgaaacc accagggacc
121cctcgacccc gctacccaca gagcctaagt ttgacatgtt gtacaagatc gaggacgtgc
181caccttggta cctgtgcatc ctgctgggct tccagcacta cctgacatgc ttcagtggta
241ccatcgccgt gcccttcctg ctggctgagg cgctgtgtgt gggccacgac cagcacatgg
301ttagtcagct catcggcacc atcttcacgt gcgtgggcat gttctgcact ctctttggca
361tgattacagc tgtggggctg tccaacctgc aatttgtgga catgaactcc tctcgcaacc
421tcttcgtgct gggattttcc atgttcttcg ggctcacgct gcccaattac ctggagtcca
481accctggcgc catcaataca ggcattcttg aagtggatca gattctgatt gtgctgctga
541ccacggagat gtttgtgggc gggtgccttg ctttcatact tgacaacaca gtgccaggga
601gcccagagga gcgtggtctg atacagtgga aagctggggc tcatgccaac agtgacatgt
661cttccagcct caagagctac gatttcccca ttgggatggg catagtaaaa agaattacct
721ttctgaaata cattcctatc tgcccagtct tcaaaggatt ttcttcaagt tcaaaagatc
781agattgcaat tccagaagac actccagaaa atacagaaac tgcatctgtg tgcaccaagg
841tctgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
901tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
961tctggatta

Download FASTA (ORF) (Full)