Construct: ORF ccsbBroad304_11654
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001231.1_s304c1, BRDN0000409059
- Derived from:
- ccsbBroadEn_11654
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCAP (22937)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11654
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22937 | SCAP | SREBF chaperone | XM_011533502.3 | 97.9% | 96.2% | (many diffs) |
| 2 | human | 22937 | SCAP | SREBF chaperone | XM_017005921.1 | 97.6% | 96.7% | (many diffs) |
| 3 | human | 22937 | SCAP | SREBF chaperone | XM_005264971.1 | 97.5% | 96.6% | (many diffs) |
| 4 | human | 22937 | SCAP | SREBF chaperone | NM_001320044.2 | 87.6% | 85.7% | (many diffs) |
| 5 | human | 22937 | SCAP | SREBF chaperone | XM_005264968.1 | 87.5% | 85.6% | (many diffs) |
| 6 | human | 22937 | SCAP | SREBF chaperone | XM_005264967.2 | 70.1% | 68.7% | (many diffs) |
| 7 | human | 22937 | SCAP | SREBF chaperone | NM_012235.4 | 70.1% | 68.6% | (many diffs) |
| 8 | human | 22937 | SCAP | SREBF chaperone | XM_011533501.1 | 70.1% | 68.6% | (many diffs) |
| 9 | human | 22937 | SCAP | SREBF chaperone | XM_017005918.1 | 70.1% | 68.6% | (many diffs) |
| 10 | mouse | 235623 | Scap | SREBF chaperone | NM_001001144.3 | 61.2% | 62.2% | (many diffs) |
| 11 | mouse | 235623 | Scap | SREBF chaperone | NM_001103162.2 | 61.2% | 62.2% | (many diffs) |
| 12 | mouse | 235623 | Scap | SREBF chaperone | XM_006512083.2 | 61.2% | 62.2% | (many diffs) |
| 13 | mouse | 235623 | Scap | SREBF chaperone | XM_006512084.3 | 61.2% | 62.2% | (many diffs) |
| 14 | mouse | 235623 | Scap | SREBF chaperone | XM_006512085.3 | 61.2% | 62.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2781
- ORF length:
- 2715
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac cctgactgaa aggctgcgtg agaagatatc tcgggccttc tacaaccatg 121 ggctcctctg tgcatcctat cccatcccca tcatcctcat cggctacttc accctagtgc 181 ccgccatcca ggagttctgt ctctttgctg tcgtggggct ggtgtctgac ttcttccttc 241 agatgctgtt tttcaccact gtcctgtcca ttgacattcg ccggatggag ctagcagacc 301 tgaacaagcg actgccccct gaggcctgcc tgccctcagc caagccagtg gggcagccaa 361 cgcgctacga gcggcagctg gctgtgaggc cgtccacacc ccacaccatc acgttgcagc 421 cgtcttcctt ccgaaacctg cggctcccca agaggctgcg tgttgtctac ttcctggccc 481 gcacccgcct ggcacagcgc ctcatcatgg ctggcaccgt tgtctggatt ggcatcctgg 541 tatacacaga cccagcaggg ctgcgcaact acctcgctgc ccaggtgacg gaacagagcc 601 cattgggtga gggagccctg gctcccatgc ccgtgcctag tggcatgctg ccccccagcc 661 acccggaccc tgccttctcc atcttcccac ctgatgcccc taagctacct gagaaccaga 721 cgtcgccagg cgagtcacct gagcgtggag gtccagcaga ggttgtccat gacagcccag 781 tcccagaggt aacctggggg cctgaggatg aggaactttg gaggaaattg tccttccgcc 841 actggccgac gctcttcagc tattacaaca tcacactggc caagaggtac atcagcctgc 901 tgcccgtcat cccagtcacg ctccgcctga acccgaggga ggctctggag ggccggcacc 961 ctcaggacgg ccgcagtgcc tggcccccac cggggcccat acctgctggg cactgggaag 1021 caggacccaa gggcccaggt ggggtgcagg cccatggaga cgtcacgctg tacaaggtgg 1081 cggcgctggg cctggccacc ggcatcgtct tggtgctgct gctgctctgc ctctaccgcg 1141 tgctatgccc gcgcaactac gggcagctgg gtggtgggcc cgggcggcgg aggcgcgggg 1201 agctgccctg cgacgactac ggctatgcgc cacccgagac ggagatcgtg ccgcttgtgc 1261 tgcgcggcca cctcatggac atcgagtgcc tggccagcga cggcatgctg ctggtgagct 1321 gctgcctggc aggccacatc tgcgtgtggg acgcgcagac cggggattgc ctaacgcgca 1381 ttccgcgccc agggcagcgc cgggacagtg gcgtgggcag cgggcttgag gctcaggaga 1441 gctgggaacg actttcagat ggtgggaagg ctggtccaga ggagcctggg gacagccctc 1501 ccctgagaca ccgcccccgg ggccctccgc cgccttccct cttcggggac cagcctgacc 1561 tcacctgctt aattgacacc aacttttcag cgcagcctcg gtcctcacag cccactcagc 1621 ccgagccccg gcaccgggcg gtctgtggcc gctctcggga ctccccaggc tatgacttca 1681 gctgcctggt gcagcgggtg taccaggagg aggggctggc ggccgtctgc acaccagccc 1741 tgcgcccacc ctcgcctggg ccggtgctgt cccaggcccc tgaggacgag ggtggctccc 1801 ccgagaaagg ctccccttcc ctcgcctggg cccccagtgc cgagggttcc atctggagct 1861 tggagctgca gggcaacctc atcgtggtgg ggcggagcag cggccggctg gaggtgtggg 1921 acgccattga aggggtgctg tgctgcagca gcgaggaggt ctcctcaggc attaccgctc 1981 tggtgttctt ggacaaaagg attgtggctg cacggctcaa cggttccctt gatttcttct 2041 ccttggagac ccacactgcc ctcagccccc tgcagtttag agggacccca gggcggggca 2101 gttcccctgc ctctccagtg tacagcagca gcgacacagt ggcctgtcac ctgacccaca 2161 cagtgccctg tgcacaccaa aaacccatca cagccctgaa agccgctgct gggcgcttgg 2221 tgactgggag ccaagaccac acactgagag tgttccgtct ggaggactcg tgctgcctct 2281 tcacccttca gggccactca ggggccatca cgaccgtgta cattgaccag accatggtgc 2341 tggccagtgg aggacaagat ggggccatct gcctgtggga tgtactgact ggcagccggg 2401 tcagccatgt gtttgctcac cgtggggatg tcacctccct tacctgtacc acctcctgtg 2461 tcatcagcag tggcctggat gacctcatca gcatctggga ccgcagcaca ggcatcaagt 2521 tctactccat tcagcaggac ctgggctgtg gtgcaagctt gggtgtcatc tcagacaacc 2581 tgctggtgac tggcggccag ggctgtgtct ccttttggga cctaaactac ggggacctgt 2641 tacagacagt ctacctgggg aagaacagtg aggcccagcc tgcccgccag atcctggtgc 2701 tggacaacgc tgccattgtc tgcaactttg gcagtgagct cagcctggtg tatgtgccct 2761 ctgtgctgga gaagctggac tgcccaactt tcttgtacaa agtggttggt aagcctatcc 2821 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 2881 ttaagtcgac aatcaacctc tggatta