Construct: ORF ccsbBroad304_11698
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015840.1_s304c1, BRDN0000408654
- Derived from:
- ccsbBroadEn_11698
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DTX4 (23220)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11698
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23220 | DTX4 | deltex E3 ubiquitin ligase 4 | NM_001300727.1 | 100% | 100% | |
| 2 | human | 23220 | DTX4 | deltex E3 ubiquitin ligase 4 | XM_006718483.3 | 100% | 100% | |
| 3 | human | 23220 | DTX4 | deltex E3 ubiquitin ligase 4 | NM_015177.2 | 82.8% | 82.8% | 1_318del |
| 4 | mouse | 207521 | Dtx4 | deltex 4, E3 ubiquitin ligase | XM_006526816.2 | 86.9% | 92.5% | (many diffs) |
| 5 | mouse | 207521 | Dtx4 | deltex 4, E3 ubiquitin ligase | NM_172442.3 | 72.1% | 76.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1608
- ORF length:
- 1539
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagtgggc atcaccatcc agcatgccta tgagaagcag cacccctgga 121 tcgacctcac ttccattggc tttagctacg taattgactt caacaccatg ggccagatca 181 accgtcagac ccagcgccaa cgccgcgtcc gccggcgcct cgacctcatc taccccatgg 241 tcacagggac cttgcctaag gctcagtcct ggccagtcag ccctgggcca gccacctcgc 301 cccccatgtc cccctgctcc tgtccccagt gtgtcttggt gatgagtgtt aaggcagccg 361 tggtcaatgg cagcactggg cccctacagc tgccagtgac ccgcaagaac atgccgcctc 421 ctggagtggt caagctaccc ccactgccag gctctggggc caagccactg gacagcacag 481 gcaccattcg aggcccactg aagaccgccc catcgcaggt gatccggaga caagcctcca 541 gcatgcccac tgggacaacc atgggctctc ctgccagtcc cccaggaccc aacagcaaga 601 ccggaagggt ggccctggcc accttgaatc gtaccaacct gcagcgactg gccattgccc 661 agtcccgggt gctgatcgcc tctggggtcc ccacagtccc agtgaagaac ctaaatgggt 721 ccagtcctgt caaccctgcc ttggcaggaa tcactgggat cctcatgagt gcagcggggc 781 tgcctgtgtg tctcaccagg ccaccaaagc tggtcctaca cccacccccc gtcagcaaga 841 gtgaaataaa atccatccca ggggtttcca acacaagccg caagaccacc aaaaaacaag 901 ccaagaaagg taaaacccca gaggaagtgc taaaaaaata tctacagaaa gtccggcacc 961 caccagatga ggactgcacc atctgtatgg aacgcctcac ggccccctca ggctacaagg 1021 gcccgcagcc tacggtaaaa cctgacctgg tagggaagct gtccagatgc ggccacgtct 1081 accacatcta ctgcttggtt gccatgtaca acaatgggaa caaggatgga agtttgcagt 1141 gtccaacctg caagaccatt tatggggtga agacaggcac ccaacctcca gggaagatgg 1201 agtaccacct catcccccac tccttgcctg gccacccaga ctgcaaaacc atccggatca 1261 tctacagcat cccccccggc attcagggac cggaacaccc gaatcctggg aagagtttca 1321 gcgcccgagg cttcccacga cactgttacc ttccggacag cgagaaaggg agaaaagttc 1381 tgaagctgct gctcgtggcc tgggatcgcc gcctcatttt tgccattggc acctccagca 1441 ccacaggcga gtcagacacc gtcatctgga atgaggtcca ccacaagaca gagtttggct 1501 ctaatctcac tggccatggc tacccagatg ccaattacct ggataatgtg ctggctgaac 1561 tggctgccca gggcatctct gaggacagca ctgcccagga gaaggacttg ccaactttct 1621 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1681 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta