Construct: ORF ccsbBroad304_11867
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000780.1_s304c1, BRDN0000398656
- Derived from:
- ccsbBroadEn_11867
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCLO (27445)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11867
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27445 | PCLO | piccolo presynaptic cytomat... | XM_017012007.1 | 1.6% | 1.3% | 1_6561del;6660_6667delTGTAAGAC;6693_7683del |
| 2 | human | 27445 | PCLO | piccolo presynaptic cytomat... | XM_017012006.2 | 1.4% | 1.1% | 1_6561del;6660_6667delTGTAAGAC;6693_8514del |
| 3 | human | 27445 | PCLO | piccolo presynaptic cytomat... | NM_014510.3 | .8% | .6% | 1_13656del;13755_13762delTGTAAGAC;13788_14805del |
| 4 | human | 27445 | PCLO | piccolo presynaptic cytomat... | NM_033026.6 | .7% | .6% | 1_13656del;13755_13762delTGTAAGAC;13788_15426del |
| 5 | human | 27445 | PCLO | piccolo presynaptic cytomat... | XR_001744643.2 | .5% | 1_15225del;15324_15331delTGTAAGAC;15357_22942del | |
| 6 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503586.3 | .8% | .8% | (many diffs) |
| 7 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | NM_001110796.2 | .7% | .6% | (many diffs) |
| 8 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503584.3 | .7% | .6% | (many diffs) |
| 9 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | NM_011995.4 | .7% | .6% | (many diffs) |
| 10 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503583.3 | .7% | .6% | (many diffs) |
| 11 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503582.3 | .7% | .6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 189
- ORF length:
- 123
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca agtattggaa tggaatggaa ttcccttgac ttctaaaaca tatgaagaag 121 ttcagagtat cattagtcag caaagtgggg aagcagaaat atgtggacct caatatgcta 181 tcagattctg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 241 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 301 tcaacctctg gatta