Construct: ORF ccsbBroad304_11986
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001432.1_s304c1, BRDN0000400810
- Derived from:
- ccsbBroadEn_11986
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLK (51426)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11986
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51426 | POLK | DNA polymerase kappa | NM_016218.3 | 53.8% | 52.1% | (many diffs) |
| 2 | human | 51426 | POLK | DNA polymerase kappa | XM_017009559.2 | 53.8% | 52.1% | (many diffs) |
| 3 | human | 51426 | POLK | DNA polymerase kappa | XM_017009560.2 | 53.8% | 52.1% | (many diffs) |
| 4 | human | 51426 | POLK | DNA polymerase kappa | XM_005248534.5 | 53% | 51.2% | (many diffs) |
| 5 | human | 51426 | POLK | DNA polymerase kappa | XM_011543463.3 | 53% | 51.2% | (many diffs) |
| 6 | human | 51426 | POLK | DNA polymerase kappa | XM_011543464.3 | 53% | 51.2% | (many diffs) |
| 7 | human | 51426 | POLK | DNA polymerase kappa | NM_001345921.1 | 46.2% | 44.5% | (many diffs) |
| 8 | human | 51426 | POLK | DNA polymerase kappa | XM_017009561.2 | 45.5% | 43.8% | (many diffs) |
| 9 | human | 51426 | POLK | DNA polymerase kappa | NM_001345922.1 | 43.5% | 41.8% | (many diffs) |
| 10 | human | 51426 | POLK | DNA polymerase kappa | XM_017009563.2 | 43.5% | 41.8% | (many diffs) |
| 11 | human | 51426 | POLK | DNA polymerase kappa | XM_011543467.3 | 42.8% | 41.1% | (many diffs) |
| 12 | human | 51426 | POLK | DNA polymerase kappa | XR_002956163.1 | 36.1% | (many diffs) | |
| 13 | human | 51426 | POLK | DNA polymerase kappa | XR_001742108.2 | 31.5% | (many diffs) | |
| 14 | human | 51426 | POLK | DNA polymerase kappa | XR_948273.3 | 30% | (many diffs) | |
| 15 | human | 51426 | POLK | DNA polymerase kappa | XR_001742105.2 | 28.5% | (many diffs) | |
| 16 | human | 51426 | POLK | DNA polymerase kappa | XR_241784.3 | 28.4% | (many diffs) | |
| 17 | human | 51426 | POLK | DNA polymerase kappa | XR_001742107.2 | 27.7% | (many diffs) | |
| 18 | human | 51426 | POLK | DNA polymerase kappa | NR_144315.1 | 13.4% | (many diffs) | |
| 19 | mouse | 27015 | Polk | polymerase (DNA directed), ... | NM_001347604.1 | 73% | 74% | (many diffs) |
| 20 | mouse | 27015 | Polk | polymerase (DNA directed), ... | NM_001347606.1 | 60.8% | 62.4% | (many diffs) |
| 21 | mouse | 27015 | Polk | polymerase (DNA directed), ... | NM_012048.3 | 47.1% | 47.6% | (many diffs) |
| 22 | mouse | 27015 | Polk | polymerase (DNA directed), ... | NM_001347602.1 | 41.1% | 40.3% | (many diffs) |
| 23 | mouse | 27015 | Polk | polymerase (DNA directed), ... | XM_006517680.3 | 40.4% | 40.4% | (many diffs) |
| 24 | mouse | 27015 | Polk | polymerase (DNA directed), ... | NM_001347603.1 | 39.2% | 40.1% | (many diffs) |
| 25 | mouse | 27015 | Polk | polymerase (DNA directed), ... | NR_144622.1 | 29.7% | (many diffs) | |
| 26 | mouse | 27015 | Polk | polymerase (DNA directed), ... | XR_001780788.1 | 27.8% | (many diffs) | |
| 27 | mouse | 27015 | Polk | polymerase (DNA directed), ... | NR_144623.1 | 24.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1482
- ORF length:
- 1416
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttagcatgga tagcacaaag gagaagtgtg acagttacaa agatgatctt ctgcttagga 121 tgggacttaa tgataataaa gcaggaatgg aaggattaga taaagagaaa attaacaaaa 181 ttataatgga agccacgaag gggtccagat tttatggaaa tgagctcaag aaagaaaagc 241 aagtcaacca acgaattgaa aatatgatgc aacaaaaagc tcaaatcacc agccaacagc 301 taagaaaagc acaattacag gttgacagat ttgcaatgga attagaacaa agccgaaatt 361 tgagcaatac catagtgcac attgacatgg atgctttcta tgcagctgta gaaatgaggg 421 acaatccaga attgaaggat aaacccattg ctgtaggatc aatgagtatg ctgtctactt 481 caaattacca tgcaaggaga tttggtgttc gtgcagccat gccaggattt attgctaaga 541 ggctgtgccc acaacttata atagtgcccc ccaactttga caaataccga gctgtgagta 601 aagaggttaa ggaaatactt gctgattatg atcccaattt tatggccatg agtcttgatg 661 aagcctactt gaatataaca aagcacttag aagaaagaca aaattggcct gaggataaaa 721 gaaggtattt catcaaaatg ggaagctctg tagaaaatga taatccagga aaggaagtta 781 ataaactgag tgagcatgaa cgatccatct ctccactact ttttgaagag agtccttctg 841 atgtgcagcc cccaggagat cctttccaag tgaactttga agaacaaaac aatcctcaaa 901 tactccaaaa ctcagttgtt tttggaacat cagcccagga agtggtaaag gaaattcgtt 961 tcagaattga gcagaaaaca acactgacag ccagtgcagg cattgcccca aatacaatgt 1021 tagcaaaagt atgcagtgat aagaataaac caaatggaca ataccaaatt cttcccaata 1081 gacaagctgt gatggacttc atcaaggatt tacccattag aaaggtttct ggaataggaa 1141 aagttacaga gaaaatgtta aaggcccttg gaattattac atgtacagaa ctttaccaac 1201 agagggcatt gctttctctc cttttctctg aaacatcttg gcattatttc cttcatatct 1261 ccttgggtct aggttcaaca cacctgacga gggatggaga gaggaaaagt atgagcgttg 1321 agaggacatt cagtgagata aataaagcgg aagagcaata cagcctatgt caagaacttt 1381 gcagtgagct tgctcaggat ctacagaaag aaagacttaa ggtgctttat tttgatatgg 1441 tgtccttagt ttttaagttt tttaatagta aaatgctacc ttacccaact ttcttgtaca 1501 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1561 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta