Construct: ORF ccsbBroad304_12064
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009203.1_s304c1, BRDN0000402902
- Derived from:
- ccsbBroadEn_12064
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NDFIP2 (54602)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12064
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54602 | NDFIP2 | Nedd4 family interacting pr... | NM_019080.2 | 72% | 2.3% | 1_282del |
| 2 | human | 54602 | NDFIP2 | Nedd4 family interacting pr... | XM_006719840.3 | 71.7% | 2.3% | 1_282del;317_318insGGT |
| 3 | human | 54602 | NDFIP2 | Nedd4 family interacting pr... | NM_001161407.1 | 66% | 2.5% | 1_282del;715_716ins60 |
| 4 | mouse | 76273 | Ndfip2 | Nedd4 family interacting pr... | NM_001190989.1 | 61.6% | 2.3% | (many diffs) |
| 5 | mouse | 76273 | Ndfip2 | Nedd4 family interacting pr... | NM_029561.4 | 61.6% | 2.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 58
- ORF end:
- 142
- ORF length:
- 84
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaatg 61 ttggcatgga tcaccaccag ccggggactg ggcgctacca ggtgcttctt aatgaagagg 121 ataactcaga atcatcggct atagagcagc cacctacttc aaacccagca ccgcagattg 181 tgcaggctgc gtcttcagca ccagcacttg aaactgactc ttcccctcca ccatatagta 241 gtattactgt ggaagtacct acaacttcag atacagaagt ttacggtgag ttttatcccg 301 tgccacctcc ctatagcgtt gctacctctc ttcctacata cgatgaagct gagaaggcta 361 aagctgctgc aatggcagct gcagcagcag aaacatctca aagaattcag gaggaagagt 421 gtccaccaag agatgacttc agtgatgcag accagctcag agtggggaat gatggcattt 481 tcatgctggc atttttcatg gcatttattt tcaactggct tggattttgt ttatccttct 541 gtatcaccaa taccatagct ggaaggtatg gtgctatctg cggatttggc ctttccttga 601 tcaaatggat ccttattgtc aggttttctg attattttac tggatatttc aatggacagt 661 attggctttg gtggatattt cttgtacttg gcctgctcct tttcttcaga ggatttgtta 721 attatctaaa agtcagaaac atgtctgaaa gtatggcagc tgctcataga acaaggtatt 781 tcttcttatt gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 841 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 901 caatcaacct ctggatta