Transcript: Mouse NM_001190989.1

Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Ndfip2 (76273)
Length:
2202
CDS:
1..1017

Additional Resources:

NCBI RefSeq record:
NM_001190989.1
NBCI Gene record:
Ndfip2 (76273)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001190989.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000174876 GCGTAATCAGAGTTACACTAT pLKO.1 1157 3UTR 100% 4.950 6.930 N Ndfip2 n/a
2 TRCN0000173783 CCACTTCAGATACTGACGTGT pLKO.1 485 CDS 100% 2.640 3.696 N Ndfip2 n/a
3 TRCN0000279355 CCACTTCAGATACTGACGTGT pLKO_005 485 CDS 100% 2.640 3.696 N Ndfip2 n/a
4 TRCN0000174516 CTTTGGTGGATATTTCTCGTA pLKO.1 889 CDS 100% 2.640 3.696 N Ndfip2 n/a
5 TRCN0000278733 CTTTGGTGGATATTTCTCGTA pLKO_005 889 CDS 100% 2.640 3.696 N Ndfip2 n/a
6 TRCN0000279356 AGTAATCCACACACGAAATAT pLKO_005 1221 3UTR 100% 15.000 12.000 N Ndfip2 n/a
7 TRCN0000365568 TATTGGCTTTGGTGGATATTT pLKO_005 883 CDS 100% 15.000 10.500 N NDFIP2 n/a
8 TRCN0000175855 GAGGTACTTCTTCCTGCTATA pLKO.1 996 CDS 100% 10.800 7.560 N Ndfip2 n/a
9 TRCN0000278925 GAGGTACTTCTTCCTGCTATA pLKO_005 996 CDS 100% 10.800 7.560 N Ndfip2 n/a
10 TRCN0000155558 CCTTCTGTATCACCAATACCA pLKO.1 758 CDS 100% 3.000 2.100 N NDFIP2 n/a
11 TRCN0000175102 GCATTCATATTCAACTGGCTT pLKO.1 724 CDS 100% 2.640 1.848 N Ndfip2 n/a
12 TRCN0000279357 GCTACCTCTCTGCCTACATAT pLKO_005 544 CDS 100% 13.200 7.920 N Ndfip2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001190989.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12064 pDONR223 100% 61.6% 2.3% None (many diffs) n/a
2 ccsbBroad304_12064 pLX_304 0% 61.6% 2.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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