Construct: ORF ccsbBroad304_12147

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015567.1_s304c1, BRDN0000461943
Derived from:
ccsbBroadEn_12147
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CRYBG2 (55057)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12147
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55057 CRYBG2 crystallin beta-gamma domai... NM_001039775.3 37% 37% 1_3135del
2 human 55057 CRYBG2 crystallin beta-gamma domai... XM_005245918.2 37% 37% 1_3135del
3 human 55057 CRYBG2 crystallin beta-gamma domai... XM_011541672.1 36.3% 36.3% 1_3135del;3470_3471ins36
4 human 55057 CRYBG2 crystallin beta-gamma domai... XM_011541673.2 35.8% 35.8% 1_3306del
5 human 55057 CRYBG2 crystallin beta-gamma domai... XR_946681.1 33.2% 1_3271del;4712_5039del;5448_5558del
6 human 55057 CRYBG2 crystallin beta-gamma domai... XR_001737260.1 33.1% 1_3271del;4205_4206ins113;5007_5117del
7 mouse 230806 Crybg2 crystallin beta-gamma domai... NM_001162970.1 34.6% 35.4% (many diffs)
8 mouse 230806 Crybg2 crystallin beta-gamma domai... XM_006538750.2 34.6% 35.4% (many diffs)
9 mouse 230806 Crybg2 crystallin beta-gamma domai... XM_006538751.3 34.6% 35.4% (many diffs)
10 mouse 230806 Crybg2 crystallin beta-gamma domai... XM_006538748.3 28.7% 29.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1917
ORF length:
1848
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaactcaga accccaggga caaagtggag tccccaaggc atcggctccc
121taaggagggt tgtctgggac tacagcaccc cggaaatcag cctcttctct gaggagggcc
181tcaaggggga gcaagtgaag ctaacagagg ccttgaagaa ttcccagggt ctggagaagc
241ccctgcaggt ggcatctgcc accgtctctg caggactgtg gctactgtac cccaaaccat
301tattcgaaga cactccctac atcctggaac ctggagagta ccccacctca gaggcctggg
361gcacatcgga ccccagcgtg ggctccctga agcccatgag attgggctgc ccaagtgtgg
421agaagccagg ggagcccagg gctgtggtgt atgaggcccc aggctttcag ggccgcagct
481gggaagtgag ccgagacatc tacaaccttc agcagccaga ggacagccag agcccccacc
541tggcctctgt ggggtccctg agagttctcg gaggctgctg ggtgggctac gagaaggagg
601gcttccgggg ccaccagtat ctgctggagg agggggaata cccagactgg tcacactggg
661gaggctatga cgagttgctg acctccctcc gggtcatccg gacggacttc ggggacccgg
721ccgtcgtgct atttgaggcc atggacttcg aggggcacgg cgtggaggtg agcaaggcat
781tgccggatgt ggagctggtg caacacggcc ccagcacaca ggccatccac gtgctcagcg
841gcgtgtgggt ggcctaccag gaggtgggct tctccgggga acagtacgtg ctggagaagg
901gcgtgtatcg taactgcgag gactggggcg ctggcaacag caccctcgcc tcgctgcagc
961cggtcctaca ggtcggggag cacgatctgc acttcgtctc aaagattcag cttttctccc
1021gccccgactt tctgggcgac cacttctctt tcgaagatga ccaggccgct ctgcccgcct
1081ccttccgacc tcagtcctgc cgggtccacg gcggcagctg gatcctgttt gatgagacga
1141acttcgaggg tgaccagcac attctctctg agggcgagtt ccccactctc acggccatgg
1201gctgcctcgc ctccacagtc ctgggctctc tccagaaggt atccctgcac ttttcagagc
1261cttccatttt cctgtatgga ctcgagtgct tcgaggggaa ggagatcgag ctcagcaggg
1321aggtgcggag cctgcaagcc gagggcttca acaaccatgt gctgtctgtg cggatcaagg
1381ggggcatttg ggtgctatgt gaacacagtg acttccgggg ccgccagtgg ctggtgggaa
1441gctgcgagat caccaactgg ctgacctaca gcggcaccca gagggtgggc tccctctacc
1501ccatcaagca gcgccgggtt tatttccgcc tctggaatgc agcactgggg ggattcctgg
1561cagtgccgga ccatgtggag gacatgaaag caggccgtgt ggtggtcgcc gacccccaag
1621ctggaggtag ctgcatctgg tactacgagg atgggctgct gaagaaccag atggccccca
1681ccatgagcct acaggtgatt ggacccccta gcccaggctc caaggtggtg ctgtgggccg
1741agagccgcct gccgcgccag acgtggagca tcagtgaatc gggccacatc tgcagccaga
1801tgttcgaagg ccagatcctg gacgtgaagg gaggccgggg ctacgaccgg gaccacgtgg
1861tgctatggga gccggatgag gacagggcat cccagatctg gactatccac gtgcttttgc
1921caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1981ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
2041tta

Download FASTA (ORF) (Full)