Construct: ORF ccsbBroad304_12156
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000095.1_s304c1, BRDN0000409016
- Derived from:
- ccsbBroadEn_12156
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG2B (55102)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12156
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55102 | ATG2B | autophagy related 2B | NM_018036.7 | 34.7% | 34.6% | 1_4068del;4700T>C |
| 2 | mouse | 76559 | Atg2b | autophagy related 2B | XM_006516361.2 | 60.6% | 63.3% | (many diffs) |
| 3 | mouse | 76559 | Atg2b | autophagy related 2B | XM_006516360.1 | 29.7% | 31% | (many diffs) |
| 4 | mouse | 76559 | Atg2b | autophagy related 2B | NM_029654.4 | 29.6% | 30.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2232
- ORF length:
- 2166
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa tctcattcag tacattgcaa gctatggtga cttgcagaca cctaacaagg 121 cagatatgaa gcctggagcc tttcaaagaa ggtctaaggt agattccagt ggtcgatcat 181 cctcacgtgg tccagtactt cctgaagcag atcaacaaat gttacgagat ctgatgagtg 241 atgctatgga ggagatcgac atgcaacaag gcacctcgtc agtaaaacca caggctaatg 301 gtgttttgga tgaaaaatct caaattcagg agccatgttg ttcagacctc ttcctgtttc 361 ctgacgagag tgggaatgta tcccaggagt ccggccccac ctatgcctca ttctctcacc 421 atttcatcag tgatgcaatg acaggtgtgc ccactgagaa tgatgacttt tgcattcttt 481 ttgcaccaaa agcagccatg caggagaagg aagaagaacc agttataaaa atcatggttg 541 atgatgcaat tgtgataaga gacaattatt tcagtctgcc cgttaataag accgatacga 601 gcaaagcccc cttacacttt cccattcctg tgattcgcta tgtggtgaag gaggtctctc 661 ttgtctggca tctttatgga ggaaaggatt ttggaacagt ccctcccact tctccggcta 721 aaagttatat tagtccccac agttcgcctt ctcacacacc cacgagacat ggacgtaata 781 cagtatgtgg gggaaaagga aggaaccatg actttttaat ggaaatacag ctaagcaagg 841 tgaagtttca gcatgaagtc tacccgccat gcaaacctga ttgtgattcc agcctctcag 901 aacacccagt ctcccggcag gtgttcattg ttcaggatct tgagattcga gatcgtttgg 961 caacatcaca aatgaataaa tttttatacc tgtattgcag taaagaaatg cctcgaaaag 1021 ctcactccaa catgttgaca gtgaaagcct tacacgtgtg tccagaatct ggcaggtccc 1081 cacaggagtg ctgcttgaga gtgtcgctga tgccgctccg cctcaatatt gaccaggatg 1141 ctttgttctt cctgaaggat ttcttcacaa gtctttctgc agaagtagag cttcaaatga 1201 ctccagatcc agaagttaaa aagtctcctg gagctgatgt cacctgcagt ttgccaaggc 1261 atttgagtac ctcaaaggag ccaaatctgg ttatttcttt ctctgggcca aaacagcctt 1321 cccaaaatga tagtgccaat tcagtggaag tggttaatgg catggaagag aagaacttct 1381 ctgctgaaga agcatctttt agggatcagc ctgtgttttt tagagaattt agattcacgt 1441 cagaagttcc cattcgactt gattatcatg gcaaacatgt atcaatggat cagggtacgc 1501 tagctgggat tttgattggt ctggctcagt taaactgctc tgaactaaag ctcaagaggc 1561 tttcctatcg acatggttta ctaggcgttg acaaattatt ctcatatgca atcactgagt 1621 ggcttaatga cattaagaag aaccagctac caggaatcct gggaggtgtt ggacctatgc 1681 attcactagt acaattagta caaggcctaa aggacttggt ctggctccca atagagcagt 1741 accggaagga tggccgcatt gtcagagggt ttcagagagg cgctgcttcc tttggtacct 1801 cgacagcgat ggctgctcta gaactcacaa acagaatggt tcaaaccata caggcagctg 1861 cagagactgc ttatgatatg gtgtctcctg gtaccctttc tatcgagccc aagaagacca 1921 aaaggtttcc tcatcaccgg ttagcccacc agccagtaga cctgagggaa ggtgtggcca 1981 aggcctacag tgttgtgaaa gagggaatca cagacacggc tcagaccatt tatgaaactg 2041 cggctcgaga acacgagagc agaggggtga ctggtgccgt gggcgaggtt ctgcgccaga 2101 ttcctccggc agtggtgaaa cctctgattg ttgccacaga agcaacgtca aacgtgctgg 2161 gtggcatgag aaaccaaatt aggccagatg tccggcaaga cgagtcacag aaatggcgcc 2221 acggggatga ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 2281 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 2341 caatcaacct ctggatta