Construct: ORF ccsbBroad304_12195
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013675.1_s304c1, BRDN0000405818
- Derived from:
- ccsbBroadEn_12195
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS53 (55275)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12195
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55275 | VPS53 | VPS53 subunit of GARP complex | XM_017024818.1 | 19.5% | 19.3% | (many diffs) |
| 2 | human | 55275 | VPS53 | VPS53 subunit of GARP complex | XM_017024817.2 | 17.7% | 17.5% | (many diffs) |
| 3 | human | 55275 | VPS53 | VPS53 subunit of GARP complex | NM_001128159.3 | 16.6% | 16.4% | (many diffs) |
| 4 | human | 55275 | VPS53 | VPS53 subunit of GARP complex | XR_001752553.2 | 8.3% | (many diffs) | |
| 5 | human | 55275 | VPS53 | VPS53 subunit of GARP complex | XR_934061.3 | 3.9% | (many diffs) | |
| 6 | mouse | 68299 | Vps53 | VPS53 GARP complex subunit | XM_006534053.3 | 17.2% | 18.3% | (many diffs) |
| 7 | mouse | 68299 | Vps53 | VPS53 GARP complex subunit | XM_006534052.3 | 15.1% | 16.1% | (many diffs) |
| 8 | mouse | 68299 | Vps53 | VPS53 GARP complex subunit | NM_026664.3 | 14.6% | 15.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 486
- ORF length:
- 420
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tttgctgctg ctggacaccc actcgctgaa gatggtcctg ctcgatctcc 121 cctccatcag ctcgcaggtg gtgaggaagg cacccgccag ctacaccaag atcgttgtca 181 aaggcatgac ccgggctgag atgatcctca aggtagtgat ggcccctcat gaaccgttgg 241 tggtgtttgt tgacaactac atcaaacttc tcacagactg caacacagaa acctttcaga 301 agatactgga catgaagggg ctgaagagga gtgagcagag cagcatgctg gaactcctgc 361 gccagcggct ccccgcaccg ccctcggggg cagaaagctc cggctcactg tccctgacgg 421 cgccgacacc agagcaagag tcgtcacgca tccgcaagct cgagaaactc attaaaaaga 481 gactgtaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 541 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 601 acctctggat ta