Construct: ORF ccsbBroad304_12494
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007952.1_s304c1, BRDN0000409658
- Derived from:
- ccsbBroadEn_12494
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D15 (64786)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12494
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | NM_022771.5 | 99.9% | 99.8% | 1621A>G |
| 2 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_006719565.4 | 98.6% | 98.4% | 0_1ins27;2T>A;1594A>G |
| 3 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_006719564.2 | 98.1% | 97.1% | (many diffs) |
| 4 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | NM_001146213.2 | 97.4% | 97.3% | 655_656ins51;1570A>G |
| 5 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | NM_001146214.2 | 95.7% | 94.7% | (many diffs) |
| 6 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_006719566.3 | 85.6% | 85.5% | 0_1ins297;1324A>G |
| 7 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_011538679.1 | 85.6% | 85.5% | 0_1ins297;1324A>G |
| 8 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_011538680.1 | 85.6% | 85.5% | 0_1ins297;1324A>G |
| 9 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_017019825.1 | 83.1% | 83% | 0_1ins297;358_359ins51;1273A>G |
| 10 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_017019827.1 | 79.1% | 79% | 0_1ins432;1189A>G |
| 11 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_017019828.1 | 76.6% | 76.5% | 0_1ins432;223_224ins51;1138A>G |
| 12 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_011538681.3 | 65.6% | 65.1% | (many diffs) |
| 13 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_006719567.2 | 64.3% | 64.2% | 0_1ins738;883A>G |
| 14 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | XM_011538682.1 | 64.3% | 64.2% | 0_1ins738;883A>G |
| 15 | human | 64786 | TBC1D15 | TBC1 domain family member 15 | NR_027449.2 | 29.4% | (many diffs) | |
| 16 | mouse | 66687 | Tbc1d15 | TBC1 domain family, member 15 | XM_006513957.3 | 87.5% | 91.1% | (many diffs) |
| 17 | mouse | 66687 | Tbc1d15 | TBC1 domain family, member 15 | NM_025706.3 | 85.1% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2139
- ORF length:
- 2073
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcgggt gttgtgagcg ggaagattat atatgaacaa gaaggagtat 121 atattcactc atcttgtgga aagaccaatg accaagacgg cttgatttca ggaatattac 181 gtgttttaga aaaggatgcc gaagtaatag tggactggag accattggat gatgcattag 241 attcctctag tattctctat gctagaaagg actccagttc agttgtagaa tggactcagg 301 ccccaaaaga aagaggtcat cgaggatcag aacatctgaa cagttacgaa gcagaatggg 361 acatggttaa tacagtttca tttaaaagga aaccacatac caatggagat gctccaagtc 421 atagaaatgg gaaaagcaaa tggtcattcc tgttcagttt gacagacctg aaatcaatca 481 agcaaaacaa agagggtatg ggctggtcct atttggtatt ctgtctaaag gatgacgtcg 541 ttctccctgc tctacacttt catcaaggag atagcaaact actgattgaa tctcttgaaa 601 aatatgtggt attgtgtgaa tctccacagg ataaaagaac acttcttgtg aattgtcaga 661 ataagagtct ttcacagtct tttgaaaatc ttcttgatga gccagcatat ggtttaatac 721 aagcaggact gctagacaga agaaagctgt tgtgggccat tcaccactgg aaaaaaatta 781 aaaaggaccc ttatacggca actatgatag gattttccaa agtcacaaac tacatttttg 841 acagtttgag aggcagcgat ccctctacac atcaacgacc accttcagaa atggcagatt 901 ttcttagtga tgctattcca ggtctaaaga taaatcaaca agaagaacca ggatttgaag 961 tcatcacaag aattgatttg ggggaacgcc ctgttgttca aaggagagaa ccggtatcac 1021 tggaagaatg gactaagaac attgattctg aaggaagaat tttaaatgta gataatatga 1081 agcagatgat atttagaggg ggacttagtc atgcattgag aaagcaagca tggaaatttc 1141 ttctgggtta ttttccctgg gacagtacca aggaggaaag aacccaatta caaaagcaaa 1201 aaactgatga atacttcaga atgaaactgc agtggaaatc catcagccag gaacaagaga 1261 aaagaaattc gaggttaaga gattatagaa gtcttatcga aaaagatgtt aacagaacag 1321 atcgaacaaa caagttttat gaaggccaag ataatccagg gttgatttta cttcatgaca 1381 ttttgatgac ctactgtatg tatgattttg atttaggata tgttcaagga atgagtgatt 1441 tactttcccc tcttttatat gtgatggaaa atgaagtgga tgccttttgg tgctttgcct 1501 cttacatgga ccaaatgcat cagaattttg aagaacaaat gcaaggcatg aagacccagc 1561 taattcagct gagtacctta cttcgattgt tagacagtgg attttgcagt tacttagaat 1621 ctcaggactc tggatacctt tatttttgct tcaggtggct tttaatcaga ttcaaaaggg 1681 aatttggttt tctagatatt cttcgattat gggaggtaat gtggaccgaa ctaccatgta 1741 caaatttcca tcttcttctc tgttgtgcta ttctggaatc agaaaagcag caaataatgg 1801 aaaagcatta tggcttcaat gaaatactta agcatatcaa tgaattgtcc atgaaaattg 1861 atgtggaaga tatactctgc aaggcagaag caatttctct acagatggta aaatgcaagg 1921 aattgccaca agcagtctgt gagatccttg ggcttcaagg cagtgaagtt acaacaccag 1981 attcagacgt tggtgaagac gaaaatgttg tcatgactcc ttgtcctaca tctgcatttc 2041 aaagtaatgc cttgcctaca ctctctgcca gtggagccag aaatgacagc ccaacacaga 2101 taccagtgtc ctcagatgtc tgcagattaa cacctgcatg cccaacttcc ttgtacaaag 2161 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2221 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta