Construct: ORF ccsbBroad304_12508
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018457.1_s304c1, BRDN0000406544
- Derived from:
- ccsbBroadEn_12508
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDRG4 (65009)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12508
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 65009 | NDRG4 | NDRG family member 4 | NM_001242836.1 | 100% | 100% | |
2 | human | 65009 | NDRG4 | NDRG family member 4 | NM_001242835.1 | 96.3% | 96% | 866_904del |
3 | human | 65009 | NDRG4 | NDRG family member 4 | NM_001242834.1 | 94.9% | 94.9% | 21_74del |
4 | human | 65009 | NDRG4 | NDRG family member 4 | NM_001242833.1 | 91.8% | 91.8% | 21_110del |
5 | human | 65009 | NDRG4 | NDRG family member 4 | XM_006721262.3 | 91.6% | 91.3% | 21_74del;920_958del |
6 | human | 65009 | NDRG4 | NDRG family member 4 | NM_020465.3 | 90.9% | 89.5% | (many diffs) |
7 | human | 65009 | NDRG4 | NDRG family member 4 | NM_022910.3 | 90.9% | 89.5% | (many diffs) |
8 | human | 65009 | NDRG4 | NDRG family member 4 | XM_017023590.1 | 90.9% | 89.5% | (many diffs) |
9 | human | 65009 | NDRG4 | NDRG family member 4 | XM_006721261.3 | 88.7% | 88.4% | 21_110del;956_994del |
10 | human | 65009 | NDRG4 | NDRG family member 4 | XM_017023589.2 | 87.8% | 86.4% | (many diffs) |
11 | human | 65009 | NDRG4 | NDRG family member 4 | XM_006721260.4 | 87.8% | 86.2% | (many diffs) |
12 | human | 65009 | NDRG4 | NDRG family member 4 | XM_011523293.2 | 87.8% | 86.2% | (many diffs) |
13 | human | 65009 | NDRG4 | NDRG family member 4 | XM_011523294.2 | 87.8% | 86.2% | (many diffs) |
14 | human | 65009 | NDRG4 | NDRG family member 4 | XM_017023582.2 | 86.6% | 85.6% | (many diffs) |
15 | human | 65009 | NDRG4 | NDRG family member 4 | XM_017023588.1 | 86.6% | 85.6% | (many diffs) |
16 | human | 65009 | NDRG4 | NDRG family member 4 | NM_001130487.1 | 86.2% | 84.9% | (many diffs) |
17 | human | 65009 | NDRG4 | NDRG family member 4 | XM_006721259.4 | 84.9% | 83.4% | (many diffs) |
18 | human | 65009 | NDRG4 | NDRG family member 4 | XM_011523292.3 | 84.2% | 82.7% | (many diffs) |
19 | human | 65009 | NDRG4 | NDRG family member 4 | XM_017023587.1 | 84.2% | 82.7% | (many diffs) |
20 | human | 65009 | NDRG4 | NDRG family member 4 | XM_017023586.1 | 83.8% | 82.8% | (many diffs) |
21 | human | 65009 | NDRG4 | NDRG family member 4 | XM_006721258.3 | 83.8% | 82.5% | (many diffs) |
22 | human | 65009 | NDRG4 | NDRG family member 4 | XM_011523291.3 | 83.8% | 82.5% | (many diffs) |
23 | human | 65009 | NDRG4 | NDRG family member 4 | NM_001363869.1 | 83.7% | 83.7% | 0_1ins165 |
24 | human | 65009 | NDRG4 | NDRG family member 4 | XM_006721257.4 | 83.4% | 81.9% | (many diffs) |
25 | human | 65009 | NDRG4 | NDRG family member 4 | XM_017023585.1 | 82.3% | 81.4% | (many diffs) |
26 | human | 65009 | NDRG4 | NDRG family member 4 | XM_006721256.3 | 81.6% | 79.9% | (many diffs) |
27 | human | 65009 | NDRG4 | NDRG family member 4 | XM_011523290.3 | 81.6% | 79.9% | (many diffs) |
28 | human | 65009 | NDRG4 | NDRG family member 4 | XM_024450398.1 | 81.6% | 79.9% | (many diffs) |
29 | human | 65009 | NDRG4 | NDRG family member 4 | XM_024450400.1 | 80.6% | 80.3% | 0_1ins165;701_739del |
30 | human | 65009 | NDRG4 | NDRG family member 4 | XM_017023584.1 | 80.2% | 78.8% | (many diffs) |
31 | human | 65009 | NDRG4 | NDRG family member 4 | XM_006721254.3 | 79.8% | 78.6% | (many diffs) |
32 | human | 65009 | NDRG4 | NDRG family member 4 | XM_006721253.3 | 79.1% | 77.5% | (many diffs) |
33 | human | 65009 | NDRG4 | NDRG family member 4 | XM_006721252.3 | 77.8% | 76.2% | (many diffs) |
34 | human | 65009 | NDRG4 | NDRG family member 4 | XM_017023583.2 | 71.3% | 70% | (many diffs) |
35 | human | 65009 | NDRG4 | NDRG family member 4 | NR_040072.1 | 30.9% | 1_106del;127_218del;1216_3282del | |
36 | mouse | 234593 | Ndrg4 | N-myc downstream regulated ... | NM_145602.3 | 91.2% | 97.9% | (many diffs) |
37 | mouse | 234593 | Ndrg4 | N-myc downstream regulated ... | NM_001195006.1 | 87.8% | 94% | (many diffs) |
38 | mouse | 234593 | Ndrg4 | N-myc downstream regulated ... | XM_006530869.1 | 80% | 84.4% | (many diffs) |
39 | mouse | 234593 | Ndrg4 | N-myc downstream regulated ... | XM_006530868.1 | 78.5% | 83.1% | (many diffs) |
40 | mouse | 234593 | Ndrg4 | N-myc downstream regulated ... | XM_006530867.1 | 76% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1083
- ORF length:
- 1017
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggagtgctgg gatggggaac atgacatcga gacaccctac ggccttctgc 121 atgtagtgat ccggggctcc cccaagggga accgcccagc catcctcacc taccatgatg 181 tgggcctcaa ccacaaacta tgcttcaaca ccttcttcaa cttcgaggac atgcaggaga 241 tcaccaagca ctttgtggtg tgtcacgtgg atgcccctgg acaacaggtg ggggcgtcgc 301 agtttcctca ggggtaccag ttcccctcca tggagcagct ggctgccatg ctccccagcg 361 tggtgcagca tttcgggttc aagtatgtga ttggcatcgg agtgggcgcc ggagcctatg 421 tgctggccaa gtttgcactc atcttccccg acctggtgga ggggctggtg ctggtgaaca 481 tcgaccccaa tggcaaaggc tggatagact gggctgccac caagctctcc ggcctaacta 541 gcactttacc cgacacggtg ctctcccacc tcttcagcca ggaggagctg gtgaacaaca 601 cagagttggt gcagagctac cggcagcaga ttgggaacgt ggtgaaccag gccaacctgc 661 agctcttctg gaacatgtac aacagccgca gagacctgga cattaaccgg cctggaacgg 721 tgcccaatgc caagacgctc cgctgccccg tgatgctggt ggttggggat aatgcacccg 781 ctgaggacgg ggtggtggag tgcaactcca aactggaccc gaccactacg accttcctga 841 agatggcaga ctctggaggg ctgccccagg tcacacagcc agggaagctg actgaagcct 901 tcaaatactt cctgcaaggc atgggctaca tgccctcagc cagcatgacc cgcctggcac 961 gctcccgcac tgcatccctc accagtgcca gctcggtgga tggcagccgc ccacaggcct 1021 gcacccactc agagagcagc gaggggctgg gccaggtcaa ccacaccatg gaggtgtcct 1081 gttgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1141 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1201 tctggatta