Transcript: Human XM_006721254.3

PREDICTED: Homo sapiens NDRG family member 4 (NDRG4), transcript variant X9, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NDRG4 (65009)
Length:
3461
CDS:
150..1418

Additional Resources:

NCBI RefSeq record:
XM_006721254.3
NBCI Gene record:
NDRG4 (65009)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006721254.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000134583 GACTGAAGCCTTCAAATACTT pLKO.1 1184 CDS 100% 5.625 3.938 N NDRG4 n/a
2 TRCN0000137674 GAAGCTGACTGAAGCCTTCAA pLKO.1 1178 CDS 100% 4.950 3.465 N NDRG4 n/a
3 TRCN0000138040 GCAGCTCTTCTGGAACATGTA pLKO.1 953 CDS 100% 4.950 3.465 N NDRG4 n/a
4 TRCN0000137216 GCTCTTCTGGAACATGTACAA pLKO.1 956 CDS 100% 4.950 3.465 N NDRG4 n/a
5 TRCN0000138797 GCCTCAACCACAAACTATGCT pLKO.1 478 CDS 100% 3.000 2.100 N NDRG4 n/a
6 TRCN0000137575 CCTAACTAGCACTTTACCCGA pLKO.1 827 CDS 100% 0.660 0.462 N NDRG4 n/a
7 TRCN0000138639 CATCCTCACCTACCATGATGT pLKO.1 455 CDS 100% 4.950 2.970 N NDRG4 n/a
8 TRCN0000133825 CAAACTATGCTTCAACACCTT pLKO.1 488 CDS 100% 2.640 1.584 N NDRG4 n/a
9 TRCN0000137231 GCCTTCAAATACTTCCTGCAA pLKO.1 1191 CDS 100% 2.640 1.584 N NDRG4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006721254.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12508 pDONR223 100% 79.8% 78.6% None (many diffs) n/a
2 ccsbBroad304_12508 pLX_304 0% 79.8% 78.6% V5 (many diffs) n/a
3 TRCN0000471852 TGATTCATGTCCCCATCGGCCGGG pLX_317 50.4% 79.8% 78.6% V5 (many diffs) n/a
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