Construct: ORF ccsbBroad304_12547
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013829.1_s304c1, BRDN0000398808
- Derived from:
- ccsbBroadEn_12547
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFAF5 (79133)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12547
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_001352406.2 | 100% | 100% | |
| 2 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_001352407.2 | 100% | 100% | |
| 3 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_001352403.2 | 84% | 84% | 1_90del |
| 4 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | XM_006723624.2 | 84% | 84% | 1_90del |
| 5 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | XM_024451999.1 | 84% | 84% | 1_90del |
| 6 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_001039375.3 | 49.8% | 49.8% | 1_477del |
| 7 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_024120.5 | 45.7% | 45.7% | 1_561del |
| 8 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | XR_937140.2 | 32.1% | (many diffs) | |
| 9 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_001352408.1 | 16.6% | 15.2% | (many diffs) |
| 10 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | XR_430269.3 | 9.8% | (many diffs) | |
| 11 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147980.2 | 8.7% | 1_498del;713_810del;1071_5443del | |
| 12 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | XR_001754396.1 | 8.6% | 1_520del;735_832del;1093_5465del | |
| 13 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147978.2 | 8.6% | 1_602del;758_813del;1133_5505del | |
| 14 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_029377.2 | 8.5% | 1_602del;817_914del;1175_5547del | |
| 15 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147979.2 | 8.5% | 1_622del;837_934del;1195_5567del | |
| 16 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147983.2 | 8.4% | 1_652del;867_964del;1225_5597del | |
| 17 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147981.2 | 8.3% | 1_736del;951_1048del;1309_5681del | |
| 18 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147982.2 | 8.2% | (many diffs) | |
| 19 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | XM_030252053.1 | 87.7% | 90.5% | (many diffs) |
| 20 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | XM_030252052.1 | 73.7% | 76% | (many diffs) |
| 21 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | XM_011239768.2 | 43.7% | 45.1% | (many diffs) |
| 22 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | NM_027093.4 | 40.1% | 41.4% | (many diffs) |
| 23 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | XR_003953716.1 | 2.8% | (many diffs) | |
| 24 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | XR_003953715.1 | 2.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 540
- ORF length:
- 474
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt tggaggcgac acactctatg aacttcggtg ttccttacag ttagcggaaa 121 cggaaaggga aggaggattt tctccacaca tttctccttt cactgctgtc aatgacctgg 181 gacatctgct tgggagagct ggctttaata ctctgactgt ggacactgat gaaattcaag 241 ttaactatcc tggaatgttt gaattgatgg aagatttaca aggtatgggt gagagtaact 301 gtgcttggaa tagaaaagcc ctgctgcatc gagacacaat gctggcagct gcggcagtgt 361 acagagaaat gtacagaaat gaagatggtt cagtacctgc tacataccag atctattaca 421 tgataggatg gaaatatcat gagtcacagg caagaccagc tgaaagaggt tccgcaactg 481 tgtcatttgg agagctagga aaaataaaca accttatgcc accggggaaa aaatcacaat 541 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 601 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 661 ggatta