Construct: ORF ccsbBroad304_12824

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015899.1_s304c1, BRDN0000408788
Derived from:
ccsbBroadEn_12824
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPIRE2 (84501)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12824
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84501 SPIRE2 spire type actin nucleation... NM_032451.2 81.3% 81.2% 1_393del;885_890delGGTCAT;2131C>T
2 mouse 234857 Spire2 spire homolog 2 (Drosophila) NM_172287.2 67.2% 68.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1812
ORF length:
1743
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggccaacaac gacagcgagg acagcggctg cggtgccgcc gatgagggct
121acgggggtcc cgaggaggag gaggaggccg agggcgtccc ccgcagcgtg cgcacctttg
181cccaggccat gcggctgtgc gcggcgcggc tgaccgaccc ccggggcgca caggcgcatt
241accaggccgt gtgccgcgcg ctcttcgtgg agacgctgga gctgcgggcc ttcctggcca
301gggtccggga ggccaaggag atgctgcaga agcttcggga ggacgagccg catctggaga
361cgcctcgggc agagctggac agcctgggtc acacagactg ggcccgactg tgggttcagc
421tcatgcggga gctccgccgc ggagtgaagc tgaagaaggt gcaagagcag gagttcaacc
481ccctccccac cgagttccag ctcacgccct tcgagatgct gatgcaggac atccgggccc
541ggaactacaa gctgcgcaag gtggatgggg acatcccgcc ccgggtgaag aaggacgctc
601acgagctcat cctggacttt atccgctcac ggcctccact gaagcaggtc tctgagaggc
661ggctgcgccc gttgccacca aagcaaaggt ccctgcatga gaagatcctg gaggagatca
721agcaggagcg gaggctgcgc ccggtgcggg gcgagggctg ggctgcccgc gggtttggct
781ctctgccctg catcctcaac gcctgctccg gagatgccaa gtccacctcc tgcatcaacc
841tgtcagtcac agatgctggg ggcagcgccc agcgcccgcg gccccgcgtg ctgctcaagg
901cgcctacctt ggctgaaatg gaagagatga atacatctga ggaagaagag tctccgtgtg
961gggaggtgac gctgaaacgg gaccgctcct tctcagagca tgacctggcc cagctccgaa
1021gtgaggtggc ctctggcctg cagtcggcca cccacccccc aggagggacg gagccaccac
1081ggccccgagc tggcagtgcg catgtgtgga ggcccggctc ccgagaccag ggtacctgtc
1141ccgcgagtgt ctctgacccc agccaccccc tactcagcaa ccggggctcc tcgggggaca
1201gacccgaggc ctccatgacc cccgatgcca aacacctgtg gctggagttc agccaccccg
1261tggagagcct ggcgctgact gtggaagagg tgatggacgt gcgccgtgtg ctggtgaagg
1321ccgagatgga aaagtttttg cagaacaagg agctcttcag cagtctgaag aaggggaaga
1381tttgctgctg ctgccgggcc aagttcccgc tgttctcgtg gccgcccagc tgtctcttct
1441gcaagagagc cgtctgcact tcctgtagca taaagatgaa gatgccttct aagaaatttg
1501gacacatccc tgtctacaca ctgggctttg agagtcctca gagggtatca gctgccaaaa
1561ccgcgccaat ccagagaaga gacatctttc agtctctgca agggccacag tggcagagcg
1621tggaggaggc gttcccccac atctactccc acggctgtgt cctgaaggat gtctgcagtg
1681agtgcaccag ctttgtggca gacgtggtgc gttccagccg caagagcgtg gacgtcctca
1741acactacgcc acgacgcagt cgccagaccc aatccctcta catccctaac accaggactt
1801ttgacttcaa gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1861tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1921caatcaacct ctggatta

Download FASTA (ORF) (Full)