Construct: ORF ccsbBroad304_12955

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012308.1_s304c1, BRDN0000399237
Derived from:
ccsbBroadEn_12955
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM67 (91147)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12955
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 91147 TMEM67 transmembrane protein 67 NM_153704.6 98.9% 98.9% 1_30del
2 human 91147 TMEM67 transmembrane protein 67 XM_006716686.4 90.7% 90.6% 1_1delAins267;6_7insA;8_9insAAACAT
3 human 91147 TMEM67 transmembrane protein 67 NM_001142301.1 90.1% 88.6% (many diffs)
4 human 91147 TMEM67 transmembrane protein 67 NR_024522.2 88.9% 1_51del;3007_3321del
5 human 91147 TMEM67 transmembrane protein 67 XR_428387.2 86% 1_71del;3027_3434del
6 human 91147 TMEM67 transmembrane protein 67 XM_024447326.1 78.8% 78.8% 0_1ins624
7 human 91147 TMEM67 transmembrane protein 67 XM_011517363.3 69.4% 69.4% 1_30del;404_405ins882
8 human 91147 TMEM67 transmembrane protein 67 XR_928360.3 66.1% 1_71del;3027_4465del
9 human 91147 TMEM67 transmembrane protein 67 XR_001745619.2 66% 1_71del;3027_4471del
10 human 91147 TMEM67 transmembrane protein 67 XR_928362.3 61.2% 1_71del;2805_2998del;3221_4828del
11 mouse 329795 Tmem67 transmembrane protein 67 NM_177861.4 83.9% 84.4% (many diffs)
12 mouse 329795 Tmem67 transmembrane protein 67 XM_006538007.3 78.7% 79.2% (many diffs)
13 mouse 329795 Tmem67 transmembrane protein 67 XM_006538008.2 70.6% 71.3% (many diffs)
14 mouse 329795 Tmem67 transmembrane protein 67 NR_110955.1 69.3% (many diffs)
15 mouse 329795 Tmem67 transmembrane protein 67 XR_001784156.1 57.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3021
ORF length:
2955
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggtttggtcc ctcttatccg cccgggccgt gaccgcgttc cttctgttgt
121tcctccctcg cttcttacag gcccagacct tctctttccc tttccagcag ccggagaagt
181gcgacaacaa ccagtacttt gatatctccg ccctctcgtg tgttccttgt ggagctaacc
241agaggcaaga tgcccgagga acttcatgtg tatgtctacc aggatttcag atgatctcta
301ataatggagg acctgctatt atttgtaaaa agtgcccaga aaacatgaaa ggtgttacag
361aagatggctg gaactgcatt tcttgcccta gtgacttaac tgccgaagga aaatgtcact
421gtcccattgg ccatatttta gtggaaagag acattaatgg aacattgttg tctcaagcaa
481cttgtgagct ctgtgatgga aatgaaaact cttttatggt agtaaatgct ttaggagaca
541ggtgcgtccg atgtgagcca acatttgtta ataccagcag gtcctgtgca tgttcagaac
601ctaacatttt aacaggggga ttatgtttca gcagcacagg gaattttcct ctacgtagaa
661tttcagctgc acgttatgga gaagttggca tgtctttaac ttcagaatgg tttgcaaagt
721atttgcaatc atcagcagct gcatgttggg tatatgccaa tctaacatct tgtcaagctc
781ttggaaatat gtgtgtgatg aacatgaatt cttacgactt tgccacattt gatgcatgtg
841gactatttca gtttatcttt gaaaatactg ctggactgag cactgttcat tctatttcat
901tttggagaca gaatcttcct tggctgtttt atggagacca gttaggatta gcacctcaag
961tgctcagttc tacctctctt cctacaaatt tcagttttaa aggagaaaac cagaatacaa
1021aactgaagtt tgttgctgct tcctatgata taagaggaaa ttttctcaag tggcaaactt
1081tagaaggagg tgttttacag ctttgtccag acacagagac aaggctaaat gctgcttatt
1141catttggaac aacctaccaa caaaattgtg agattcctat ctctaagatc ttaattgact
1201ttcccactcc tatattttat gatgtgtacc ttgaatatac tgatgaaaat caacatcaat
1261atattttggc tgtgcctgtg ttaaacctaa atcttcaaca taataagata tttgtgaacc
1321aagacagcaa ctctggaaag tggcttctaa ctcggcgcat tttcttagtg gatgcagtaa
1381gtggacgaga aaatgactta ggaactcagc caagagtaat tcgagttgct actcaaatat
1441cactgagtgt ccaccttgta cccaacacaa taaatggaaa catctaccct cccttaatca
1501ccattgccta cagtgacatt gatatcaaag atgccaacag ccagtctgtg aaggtttctt
1561tctcagtcac atatgaaatg gatcatggag aagcacatgt ccagacagat attgctttgg
1621gtgtattggg tgggctagct gttttagcat ctcttttgaa gacagcagga tggaagaggc
1681gcattgggag tcccatgatt gatttacaga cagttgtgaa attcttggtg tactatgctg
1741gtgatctggc caatgttttc tttatcatca cagtgggaac aggtctttac tggcttattt
1801tcttcaaagc acagaagtct gtgtctgttt tgctgccaat gccaattcag gaagaacgtt
1861ttgtcactta tgttggatgt gcctttgctc tgaaggcact acaatttttg cataagctca
1921tatcccagat tacaatagat gtattcttta ttgattggga gcgacctaaa ggaaaggttc
1981ttaaagctgt tgaaggtgag ggtggtgtac gaagtgccac tgttcctgta agcatatgga
2041gaacatattt tgtagcaaat gaatggaatg aaattcagac tgtgagaaaa attaattcac
2101tctttcaagt acttactgtc ctcttctttt tggaggttgt gggattcaag aacttagcat
2161taatggactc atcttctagt ctttctagaa acccacctag ctacatagct ccttatagct
2221gcattttgag atatgcagtg tctgctgctc tttggctagc cattggaatt atacaggtcg
2281tgttctttgc tgtcttttat gagagattta tagaagataa aattcgacag ttcgttgatt
2341tatgctctat gagtaatata tcagtgtttc tgttatccca caaatgtttt ggatattaca
2401ttcatggtag atcagtacat gggcatgcag atactaatat ggaagaaatg aatatgaacc
2461ttaaaagaga agcggaaaat ttgtgtagcc agagaggttt ggtacccaac acagatggtc
2521agacttttga gattgcaatt tctaaccaga tgagacaaca ttatgacaga attcatgaga
2581cactaataag gaaaaatggt cctgctagac tactgagttc atcagcaagt acttttgagc
2641agagtataaa agcatatcat atgatgaata aatttcttgg ctccttcatt gaccatgttc
2701ataaggaaat ggattacttt ataaaagata agttgcttct tgaaagaatt cttggaatgg
2761aattcatgga accaatggaa aaaagcatct tttacaatga tgaaggttat tctttcagca
2821gtgtcctgta ttatggaaat gaagctactc ttcttatttt tgatctgctg ttcttctgtg
2881ttgtggattt ggcttgccaa aattttattt tagcatcctt ccttacatat ctacaacaag
2941agatttttag atatatccgt aatacagtag gacaaaagaa tttggcatcc aaaacattgg
3001tggatcaaag atttttgatt tacccaactt tcttgtacaa agtggttggt aagcctatcc
3061ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
3121ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)