Construct: ORF ccsbBroad304_12959
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018087.1_s304c1, BRDN0000400729
- Derived from:
- ccsbBroadEn_12959
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COL23A1 (91522)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12959
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91522 | COL23A1 | collagen type XXIII alpha 1... | XM_017010018.1 | 61.3% | 48.9% | (many diffs) |
| 2 | human | 91522 | COL23A1 | collagen type XXIII alpha 1... | NM_173465.4 | 47.2% | 39.1% | (many diffs) |
| 3 | human | 91522 | COL23A1 | collagen type XXIII alpha 1... | XM_011534688.2 | 45.9% | 38% | (many diffs) |
| 4 | human | 91522 | COL23A1 | collagen type XXIII alpha 1... | XM_006714933.3 | 45.2% | 37.4% | (many diffs) |
| 5 | human | 91522 | COL23A1 | collagen type XXIII alpha 1... | XM_011534690.2 | 44.3% | 36.4% | (many diffs) |
| 6 | human | 91522 | COL23A1 | collagen type XXIII alpha 1... | XM_006714934.3 | 43.6% | 35.9% | (many diffs) |
| 7 | human | 91522 | COL23A1 | collagen type XXIII alpha 1... | XM_006714935.3 | 43.1% | 35.4% | (many diffs) |
| 8 | human | 91522 | COL23A1 | collagen type XXIII alpha 1... | XM_011534692.2 | 41.4% | 37.2% | (many diffs) |
| 9 | human | 91522 | COL23A1 | collagen type XXIII alpha 1... | XM_011534691.2 | 41.2% | 33.3% | (many diffs) |
| 10 | human | 91522 | COL23A1 | collagen type XXIII alpha 1... | XM_011534689.2 | 41.2% | 38.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 993
- ORF length:
- 927
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gtctcgctct ggcatccagg ctggagtgcg gtgtcgcgat ctcggctcac 121 tgcaacctcc gcctctcgcg ttaaagcagt tctccagcct cagtctccca agcagctggg 181 attataggcg cctgccacca cgctgtggct tcctttttga agtcagcgag accacgaacc 241 caccggcagg aaccaactcc agacacactt tgaccgtgaa gttggacgga ctagcgaaga 301 tccggactgc tcgggaagct ccatccgaat gtgtctgccc cccagggccc cctggacggc 361 gcggcaagcc tgggagaaga ggcgaccctg gtcctccagg acccctgggt ttggatggca 421 agcccggact tccaggcccg aaaggggaaa agggtgcacc aggagacttt ggcccccggg 481 gagaccaagg acaagatgga gctgctgggc ctccggggcc ccctggacct cctggggccc 541 ggggccctcc tggcgacact gggaaagatg gccccagggg agcacaaggc ccagcgggcc 601 ccaaaggaga gcccggacaa gacggcgaga tgggcccaaa gggaccccca gggcccaagg 661 gtgagcctgg agtacctgga aagaagggcg acgatgggac accaagccag cctggaccac 721 cagggcccaa gggcgagcca gggagcatgg ggcctcgggg agagaacggt gtggacggtg 781 ccccaggacc gaagggggag cctggccacc gaggcacgga tggagctgca gggccccggg 841 gtgacgtccg ggatcctggc ctcggctctg tctcctcatg ctcccagagg ctggcttctt 901 cctccaaaaa aaatggatct gagcccccgc ctggctgtgc agggtgcccc aggcctcaag 961 ggcgagcagg gagacacagt ggtgatcgac tatgcccaac tttcttgtac aaagtggttg 1021 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1081 accggtggcg cgttaagtcg acaatcaacc tctggatta