Transcript: Human XM_017010018.1

PREDICTED: Homo sapiens collagen type XXIII alpha 1 chain (COL23A1), transcript variant X10, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
COL23A1 (91522)
Length:
1214
CDS:
89..1006

Additional Resources:

NCBI RefSeq record:
XM_017010018.1
NBCI Gene record:
COL23A1 (91522)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017010018.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000250114 GTTGGACGGACTAGCGAAGAT pLKO_005 385 CDS 100% 4.950 6.930 N COL23A1 n/a
2 TRCN0000250115 GGAAGCTCCATCCGAATGTGT pLKO_005 418 CDS 100% 3.000 4.200 N COL23A1 n/a
3 TRCN0000119431 CCAGGGCAATCAGGACGAGAT pLKO.1 500 CDS 100% 1.350 1.890 N Col23a1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017010018.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12959 pDONR223 100% 61.3% 48.9% None (many diffs) n/a
2 ccsbBroad304_12959 pLX_304 0% 61.3% 48.9% V5 (many diffs) n/a
3 TRCN0000468769 TGGATTTATTACCCTCACACCCTA pLX_317 44.8% 61.3% 48.9% V5 (many diffs) n/a
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