Construct: ORF ccsbBroad304_13168
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017528.1_s304c1, BRDN0000410864
- Derived from:
- ccsbBroadEn_13168
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL32P3 (132241)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13168
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6161 | RPL32 | ribosomal protein L32 | NM_000994.4 | 23.4% | 23.7% | (many diffs) |
| 2 | human | 6161 | RPL32 | ribosomal protein L32 | NM_001007073.1 | 23.4% | 23.7% | (many diffs) |
| 3 | human | 6161 | RPL32 | ribosomal protein L32 | NM_001007074.1 | 23.4% | 23.7% | (many diffs) |
| 4 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NM_001300976.2 | 9.5% | 8.4% | (many diffs) |
| 5 | human | 132241 | RPL32P3 | ribosomal protein L32 pseud... | NR_003111.2 | 4.6% | 1_437del;534_534delAinsCG;536_2079del | |
| 6 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NR_125355.2 | 2.5% | (many diffs) | |
| 7 | mouse | 621697 | Rpl32l | ribosomal protein L32-like | NM_001101561.2 | 21.8% | 24% | (many diffs) |
| 8 | mouse | 19951 | Rpl32 | ribosomal protein L32 | NM_172086.2 | 21.7% | 23.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 165
- ORF length:
- 99
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgccctcaga tcccttgtga agcccaagat cgtcaaaaag agaaccaaga 121 aattcatccg gcaccagtca gaccgatatg tcaaaatcaa gcgttaccca actttcttgt 181 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 241 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a