Construct: ORF ccsbBroad304_13170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011507.1_s304c1, BRDN0000410833
- Derived from:
- ccsbBroadEn_13170
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCLT1 (132320)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13170
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 132320 | SCLT1 | sodium channel and clathrin... | NM_001300897.2 | 100% | 100% | |
| 2 | human | 132320 | SCLT1 | sodium channel and clathrin... | NM_001300898.2 | 59.7% | 59.5% | 235_236delAAinsGC;238_393del |
| 3 | human | 132320 | SCLT1 | sodium channel and clathrin... | XM_017007718.2 | 11.9% | 11.8% | 235_236delAAinsGC;238_1971del |
| 4 | human | 132320 | SCLT1 | sodium channel and clathrin... | XM_017007717.2 | 11.4% | 11.4% | 235_236delAAinsGC;238_2052del |
| 5 | human | 132320 | SCLT1 | sodium channel and clathrin... | NM_144643.4 | 11.3% | 11.3% | 235_236delAAinsGC;238_2064del |
| 6 | human | 132320 | SCLT1 | sodium channel and clathrin... | XM_017007716.2 | 11.3% | 11.2% | 235_236delAAinsGC;238_2076del |
| 7 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741126.2 | 10.1% | 1_477del;712_713delAAinsGC;715_2318del | |
| 8 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741131.2 | 9.3% | 1_477del;712_713delAAinsGC;715_2505del | |
| 9 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741129.2 | 9.1% | 1_477del;712_713delAAinsGC;715_2576del | |
| 10 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_427528.3 | 8.2% | 1_477del;712_713delAAinsGC;715_2835del | |
| 11 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741128.2 | 8.2% | 1_477del;712_713delAAinsGC;715_2847del | |
| 12 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741130.2 | 8% | 1_477del;712_713delAAinsGC;715_2931del | |
| 13 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_002959708.1 | 7.7% | 1_477del;712_713delAAinsGC;715_3025del | |
| 14 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741127.2 | 7.4% | 1_477del;712_713delAAinsGC;715_3139del | |
| 15 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_938689.2 | 7.3% | 1_477del;712_713delAAinsGC;715_3218del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 303
- ORF length:
- 237
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcagaaatc gactttctga gagagcaaaa tcgaagacta aatgaagatt 121 ttaggcggta tcaaatggaa agtttttcca aatattcatc tgtacagaaa gctgtctgcc 181 aaggagaagg agacgacaca tttgaaaacc tagtatttga ccaaagcttt ttagctcctc 241 ttgttactga gtatgataaa cacctaggag aactaaatgg gcagctgaaa tattaccagg 301 catacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 361 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 421 tctggatta