Transcript: Human XR_427528.3

PREDICTED: Homo sapiens sodium channel and clathrin linker 1 (SCLT1), transcript variant X9, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SCLT1 (132320)
Length:
2835
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_427528.3
NBCI Gene record:
SCLT1 (132320)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_427528.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423027 GAGGTAGGAACTGACATATAT pLKO_005 829 3UTR 100% 15.000 21.000 N SCLT1 n/a
2 TRCN0000412856 GCCAAATTGAACGAGTCATTA pLKO_005 1715 3UTR 100% 13.200 18.480 N SCLT1 n/a
3 TRCN0000429771 ATATGACTGAGGCCCAGATTC pLKO_005 980 3UTR 100% 10.800 15.120 N SCLT1 n/a
4 TRCN0000424884 TGTACAAGATGCTACCATAAG pLKO_005 1590 3UTR 100% 10.800 15.120 N SCLT1 n/a
5 TRCN0000137249 GCCAACCAATTAAGAACCGAA pLKO.1 1369 3UTR 100% 2.640 3.696 N SCLT1 n/a
6 TRCN0000414063 CAGACTTCAAAGGCGTCTAAG pLKO_005 2231 3UTR 100% 10.800 8.640 N SCLT1 n/a
7 TRCN0000435022 GAAGCATCAGATAGGCGTTTA pLKO_005 1288 3UTR 100% 10.800 8.640 N SCLT1 n/a
8 TRCN0000415145 GCAGTTACAGTCTAGTATAAA pLKO_005 1311 3UTR 100% 15.000 10.500 N SCLT1 n/a
9 TRCN0000421651 TGACCACTTCCTGTAAGTAAA pLKO_005 2511 3UTR 100% 13.200 9.240 N SCLT1 n/a
10 TRCN0000431350 TCAATAAAGGAACGTGGATTT pLKO_005 1941 3UTR 100% 10.800 7.560 N SCLT1 n/a
11 TRCN0000134982 CACTCACAGAACTTTAGAGTT pLKO.1 2366 3UTR 100% 4.950 3.465 N SCLT1 n/a
12 TRCN0000137303 GAAGAACTTTCAGCCCTTCAA pLKO.1 1672 3UTR 100% 4.950 3.465 N SCLT1 n/a
13 TRCN0000137221 GCAGTTGGACTAGAAGTGTAT pLKO.1 2393 3UTR 100% 4.950 3.465 N SCLT1 n/a
14 TRCN0000134808 CAGAACTTTAGAGTTGGGAAA pLKO.1 2372 3UTR 100% 4.050 2.835 N SCLT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_427528.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13170 pDONR223 100% 8.2% None 1_477del;712_713delAAinsGC;715_2835del n/a
2 ccsbBroad304_13170 pLX_304 0% 8.2% V5 1_477del;712_713delAAinsGC;715_2835del n/a
3 TRCN0000466235 TGCCGGTGGAACGACGATTACGGG pLX_317 100% 8.2% V5 1_477del;712_713delAAinsGC;715_2835del n/a
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