Construct: ORF ccsbBroad304_13293
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002886.1_s304c1, BRDN0000403493
- Derived from:
- ccsbBroadEn_13293
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM81B (153643)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13293
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153643 | FAM81B | family with sequence simila... | NM_152548.3 | 91.5% | 91.5% | 1_114del;810A>T |
| 2 | human | 153643 | FAM81B | family with sequence simila... | XM_017009118.1 | 89% | 89.1% | 0_1ins135;561A>T |
| 3 | human | 153643 | FAM81B | family with sequence simila... | XM_011543209.2 | 85.9% | 85.7% | 0_1ins169;3_4insTTGAC;522A>T |
| 4 | human | 153643 | FAM81B | family with sequence simila... | XR_002959845.1 | 75% | (many diffs) | |
| 5 | human | 153643 | FAM81B | family with sequence simila... | XR_246533.3 | 70.8% | (many diffs) | |
| 6 | human | 153643 | FAM81B | family with sequence simila... | XM_005271905.2 | 64.1% | 56.7% | 0_1ins325;96_97ins119;252A>T |
| 7 | human | 153643 | FAM81B | family with sequence simila... | XM_011543210.2 | 64.1% | 56.7% | 0_1ins325;96_97ins119;252A>T |
| 8 | human | 153643 | FAM81B | family with sequence simila... | XM_011543207.1 | 59.4% | 57% | (many diffs) |
| 9 | human | 153643 | FAM81B | family with sequence simila... | XM_024454381.1 | 58.5% | 57% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1308
- ORF length:
- 1242
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ttcagataca aatgtaaaca aaagtgcctc tccaactgcg actgcagagg 121 aacagccagt tgaacctgat ggcccccttc ctggctcaga caataaccaa gaaaagaaag 181 taagattatc tccagccaaa atgtcaacca agaattctac agatctagtt gaatatgttg 241 acaagagtca tgcttttctc cccatcattc caaacaccca gagaggtcag ctagaagaca 301 gactgaacaa ccaggcgcgt accatagctt tccttcttga acaagccttc cgcatcaagg 361 aggacatctc tgcttgcctg caggggaccc atggctttcg aaaagaggaa tcgctcgcca 421 ggaagttact ggaaagccac atccagacca tcaccagcat cgtcaaaaaa ctcagccaaa 481 atattgagat tttagaagac caaataagag ctcgagatca ggcggccaca ggaactaact 541 ttgcagtaca cgagataaac atcaaacacc tacaaggagt tggagatctt cgaggaagag 601 tagccagatg tgattcaagc attgtgaagc tttctggaga cattcactta ttcaggcaag 661 agcaccggca aattgagaaa gccattcaag aattcgtgcc cgccctggaa actctttcca 721 agaacttgga catgaaggtg atgcagctct taggaaagat tgaaactgcc agttctgagc 781 aaacctcgaa tttaaagatg gtccaggggg attatcgcca cgaaatgaac cttttggaat 841 tcaaatttca ttcactttca agtaatctgt acgaagaagt tgagaataat aaaaaatgga 901 cagaaaacca atttctcaaa tatagaaaag accacctggg ccatataaat gaatgtctga 961 aggtcctaca ggagaaactg gaaaagtctg aaaataaaat ggaagaaaaa ctgctgcagc 1021 tttcaagcaa agtagagaat ttcattaaca cacagaaaca ggaaacacaa ctaagtaaag 1081 taaagcatat ggaaaataaa ttgtccaaaa agatggaaca aatggaaaag cagatctggg 1141 gtgaattaga gacaatgcag aatgaatatc aatcaggatt taaatcaatt catgactctc 1201 tcagctccct ccaacaaata cagaaaacaa agatggattt agagaaatat aaagtacaga 1261 aagacctaaa gaaattacag cgcaagatag tggaactcca ggaagtatac ccaactttct 1321 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1381 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta