Construct: ORF ccsbBroad304_13295
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015789.1_s304c1, BRDN0000411713
- Derived from:
- ccsbBroadEn_13295
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMOT (154796)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13295
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154796 | AMOT | angiomotin | NM_133265.3 | 99.8% | 99.8% | 1853_1854insTCC |
| 2 | human | 154796 | AMOT | angiomotin | XM_005262090.1 | 99.8% | 99.8% | 1853_1854insTCC |
| 3 | human | 154796 | AMOT | angiomotin | XM_017029289.1 | 99.8% | 99.8% | 1853_1854insTCC |
| 4 | human | 154796 | AMOT | angiomotin | NM_001113490.1 | 62.2% | 62.2% | 1_1227del;3080_3081insTCC |
| 5 | human | 154796 | AMOT | angiomotin | XM_005262087.1 | 62.2% | 62.2% | 1_1227del;3080_3081insTCC |
| 6 | human | 154796 | AMOT | angiomotin | XM_011530875.2 | 62.2% | 62.2% | 1_1227del;3080_3081insTCC |
| 7 | mouse | 27494 | Amot | angiomotin | XM_006528867.3 | 70.4% | 72% | (many diffs) |
| 8 | mouse | 27494 | Amot | angiomotin | NM_153319.3 | 52.4% | 53.6% | (many diffs) |
| 9 | mouse | 27494 | Amot | angiomotin | XM_006528864.3 | 52.4% | 53.6% | (many diffs) |
| 10 | mouse | 27494 | Amot | angiomotin | XM_011247828.2 | 52.4% | 53.6% | (many diffs) |
| 11 | mouse | 27494 | Amot | angiomotin | NM_001290274.1 | 36.9% | 38.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2097
- ORF length:
- 2028
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctcgggct cagccatcct ctgcttctta tcagccagtg ccagcagacc 121 cttttgccat tgtttccaga gcccagcaga tggttgagat cctctcagac gagaaccgga 181 acttgaggca agagttggaa ggatgctatg agaaggtggc aagactgcag aaggtggaga 241 cagaaatcca gcgcgtctcg gaggcatatg agaacctcgt gaagtcatcc tccaaaagag 301 aggccctaga gaaagccatg agaaacaagc tagagggcga gattcggagg atgcatgatt 361 tcaacaggga tctgagagag cgtctagaga ctgccaacaa gcagcttgca gagaaggaat 421 atgaggggtc agaggacacc agaaaaacca tctcgcagct ctttgcaaaa aataaagaaa 481 gccagcgtga gaaggagaag ctggaagcgg agctggccac tgcccgttct accaatgagg 541 accaaagacg acacatcgaa atccgagatc aggccctgag taatgcccag gccaaggtgg 601 taaagctgga agaagagctg aaaaagaagc aagtgtacgt tgacaaggtg gagaagatgc 661 agcaggccct tgtacagctc caggcagcat gtgaaaaacg tgagcagcta gagcaccgtc 721 tccggacacg actggagagg gaactggaat ccctgagaat ccagcagcgt cagggcaact 781 gtcagcccac caacgtttca gaatacaatg ctgctgcact gatggagctc cttcgggaga 841 aagaggagag gattctggct ctggaagctg atatgacaaa gtgggagcag aaatatttgg 901 aggagaatgt gatgagacat tttgctctgg atgctgctgc aactgtggct gctcagaggg 961 acacaacagt catcagtcac tctcctaaca ccagctatga cacagctcta gaagctcgca 1021 tccagaaaga ggaggaagaa atcttgatgg ccaataagcg ttgccttgac atggagggca 1081 ggattaagac cctccatgcc cagattattg agaaggatgc catgatcaaa gtactccagc 1141 agcgttcccg gaaggagccg agcaagacag agcagctgtc gtgcatgcgg ccagcgaagt 1201 ctctgatgtc catttccaat gctggatcag gcttgctctc ccactcatcc accctgactg 1261 gctcccccat catggaagaa aagcgagacg acaagagctg gaaggggagc ctaggcattc 1321 tcctgggtgg agactaccgt gctgaatatg tcccttccac accctcgcct gtgccaccct 1381 cgactcccct gctctcggct cactccaaga caggcagccg agactgcagt acccaaactg 1441 aacgtgggac ggaatcgaac aaaactgcag ctgttgctcc catctctgtt cctgctccag 1501 ttgctgctgc cgccactgct gccgccatca ctgccactgc tgccaccatc accaccacca 1561 tggtagctgc tgctccagtt gctgttgctg ctgctgctgc tccagctgct gctgctgccc 1621 cgtctccagc cactgccgct gctactgctg ctgctgtttc tccagctgct gctggtcaga 1681 ttccagctgc tgcctctgtt gcctcagctg ctgccgttgc tccttctgct gctgctgctg 1741 ctgctgttca ggttgctcca gctgctccgg ctccagttcc agctccggct ctggttccgg 1801 ttccagctcc agcagcggct caggcttctg ctcctgctca gactcaggca ccaacttcag 1861 ctccggctgt ggctccaact ccagctccaa ctccaactcc agctgtggct caggctgagg 1921 ttcctcctgc aagtccagct accggtcctg gaccacatcg tttgtctata ccaagtttga 1981 cctgcaatcc agacaaaaca gatgggcctg tgttccactc caatactctg gaaagaaaaa 2041 ctcccattca gatcctggga caagagcctg atgcagagat ggtggaatat ctcatcttgc 2101 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2161 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2221 tta