Construct: ORF ccsbBroad304_13842

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007214.1_s304c1, BRDN0000409524
Derived from:
ccsbBroadEn_13842
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
DACH1 (1602)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13842
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1602 DACH1 dachshund family transcript... NM_080759.6 73.1% 2.4% 1_567del;580_581insG
2 human 1602 DACH1 dachshund family transcript... XM_011534942.3 70.5% 6% 0_1ins457
3 human 1602 DACH1 dachshund family transcript... NM_001366712.1 68.1% 2.2% 1_567del;580_581insG;1126_1281del
4 human 1602 DACH1 dachshund family transcript... XM_017020396.1 64.1% 4.6% 0_1ins457;103_258del
5 human 1602 DACH1 dachshund family transcript... NM_080760.5 52.2% 3% 1_567del;580_581insG;1125_1126ins444
6 human 1602 DACH1 dachshund family transcript... XM_011534940.2 46.4% 2.8% (many diffs)
7 human 1602 DACH1 dachshund family transcript... NM_004392.6 44.5% 3.3% 1_567del;580_581insG;962_963ins606
8 human 1602 DACH1 dachshund family transcript... XM_011534941.2 26.7% 4.4% (many diffs)
9 mouse 13134 Dach1 dachshund 1 (Drosophila) NM_001038610.2 68.4% 3% (many diffs)
10 mouse 13134 Dach1 dachshund 1 (Drosophila) NM_007826.3 63.7% 2.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
330
ORF length:
264
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt ggatctgagg gggggccaaa gtggcttcct tcacggtgga gggctgcgag
121ctgatctgcc tgccccaggc tttcgacctg ttcctgaagc acttggtggg gggcttgcat
181acggtctaca ccaagctgaa gcggctggag atcacgccgg tggtgtgcaa tgtggaacaa
241gttcgcatcc tgaggggact gggcgccatc cagccaggag tgaaccgctg caaactcatc
301tccaggaagg acttcgagac cctctacaat gactgcacca acgcaagttc tagacctgga
361aggcctccta agaggactca aagtgtcacc tccccagaga actctcacat catgccgcat
421tctgtccctg gtctcatgtc tcctgggata attccaccaa caggtctgac agcagccgct
481gcagcagctg ctgctgctac caatgcagct attgctgaag caatgaaggt gaaaaaaatc
541aaattagaag ccatgagcaa ctatcatgcc agtaataacc aacatggagc agactctgaa
601aacggggaca tgaattcaag tgtcggactg gaacttcctt ttatgatgat gccccaccct
661ctaattcctg tcagcctacc tccagcatct gtcaccatgg caatgagcca gatgaaccac
721ctcagcacca ttgcaaatat ggcagcagca gcacaagttc agagtccccc atccagagtt
781gagacatcag ttattaagga gcgtgttcct gatagcccct cacctgcccc ctctctggag
841gaggggagaa ggcctggcag tcacccatca tcacatcgca gcagcagcgt gtccagctcc
901cctgctcgga ctgagagctc ttctgacaga atcccggtcc atcagaatgg gttgtccatg
961aaccagatgc tgatgggctt atcaccaaat gtacttcctg ggcccaaaga gggagatttg
1021gccggtcatg acatgggaca tgagtcaaaa aggatgcata ttgaaaaaga tgagaccccg
1081ctttctacac caaccgcaag agacagcctt gacaaactct ctctaactgg gcatggacaa
1141ccactgcctc caggttttcc atctcctttt ctgtttcctg atggactgtc ttccatcgag
1201actcttctga ctaacataca ggggctgttg aaagttgcca tagataatgc cagagctcaa
1261gagaaacagg tccaactgga aaaaactgag ctgaagatgg attttttaag ggaaagagaa
1321ctaagggaaa cacttgagaa gcagttggct atggaacaaa agaatagagc catagttcaa
1381aagaggctaa agaaggagaa gaaggcaaag agaaaattgc aggaagcact tgagtttgag
1441acgaaacggc gtgaacaagc agaacagacg ctaaaacagg cagcttcaac agatagtctc
1501agggtcttaa atgactctct gaccccagag atagaggctg accgcagtgg cggcagaaca
1561gatgctgaaa ggacaataca agatggaaga ctgtatttga aaactactgt catgtactgc
1621ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat
1681tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg
1741atta

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