Construct: ORF ccsbBroad304_14064
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011571.1_s304c1, BRDN0000401026
- Derived from:
- ccsbBroadEn_14064
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NUDT4 (11163)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14064
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_019094.6 | 99.4% | 46.6% | 254_254delAinsNAN |
| 2 | human | 440672 | NUDT4B | nudix hydrolase 4B | NM_001355407.1 | 99.2% | 46.4% | 253_254insN;255_257delGCAinsN |
| 3 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_199040.4 | 99.2% | 46.4% | 253_254insN;255_257delGCAinsN |
| 4 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_001301023.2 | 70.6% | 17.7% | 0_1ins156;98_98delAinsNAN |
| 5 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_001301024.2 | 70.6% | 17.7% | 0_1ins156;98_98delAinsNAN |
| 6 | human | 11163 | NUDT4 | nudix hydrolase 4 | XM_011537795.2 | 70.6% | 17.7% | 0_1ins156;98_98delAinsNAN |
| 7 | human | 11163 | NUDT4 | nudix hydrolase 4 | XM_011537796.2 | 70.6% | 17.7% | 0_1ins156;98_98delAinsNAN |
| 8 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_001301022.2 | 70.5% | 18% | 0_1ins156;97_98insN;99_101delGCAinsN |
| 9 | human | 11163 | NUDT4 | nudix hydrolase 4 | XM_011537794.2 | 70.5% | 18% | 0_1ins156;97_98insN;99_101delGCAinsN |
| 10 | human | 170688 | NUDT4P2 | nudix hydrolase 4 pseudogene 2 | NR_104005.1 | 14% | (many diffs) | |
| 11 | mouse | 71207 | Nudt4 | nudix (nucleoside diphospha... | NM_027722.4 | 89.2% | 46.1% | (many diffs) |
| 12 | mouse | 71207 | Nudt4 | nudix (nucleoside diphospha... | XM_006514116.2 | 89.1% | 45.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 360
- ORF length:
- 294
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat gaagttcaag cccaaccaga cgcggaccta cgaccgcgag ggcttcaaga 121 agcgggcggc gtgcctgtgc ttccggagcg agcaggagga cgaggtgctg ctggtgagta 181 gcagccggta cccagaccag tggattgtcc caggaggagg aatggaaccc gaggaggaac 241 ctggcggtgc tgccgtgagg gaagtttatg aggaggctgg agtcaaagga aaactaggca 301 gacttctggg catatttgna ngaaccaaga ccgaaagcac agaacatatg tttatgttct 361 aacagtcact gaaatattag aagattggga agattctgtt aatattggaa ggaagagaga 421 gtggttcaaa gtagaagatg ctatcaaagt tctccagtgt cataaacctg tacatgcaga 481 gtatctggaa aagctaaagc tgggttgttc cccagccaat ggaaattcta cagtcccttc 541 ccttccggat aataatgcct tgtttgtaac cgctgcacag acctctgggt tgccatctag 601 tgtaagatac ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct 661 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat 721 caacctctgg atta