Construct: ORF ccsbBroad304_14073
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010149.1_s304c1, BRDN0000399960
- Derived from:
- ccsbBroadEn_14073
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- KIN (22944)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14073
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22944 | KIN | Kin17 DNA and RNA binding p... | NM_012311.4 | 99.9% | 3.4% | 3delG |
| 2 | human | 22944 | KIN | Kin17 DNA and RNA binding p... | XM_006717434.4 | 95.9% | 3.4% | (many diffs) |
| 3 | human | 22944 | KIN | Kin17 DNA and RNA binding p... | XM_011519427.2 | 94.9% | 3.5% | 3delG;1121delT;1122_1123ins58 |
| 4 | human | 22944 | KIN | Kin17 DNA and RNA binding p... | XM_017015946.1 | 73% | 2.4% | 0_1ins317 |
| 5 | human | 22944 | KIN | Kin17 DNA and RNA binding p... | XM_006717435.4 | 69.5% | 7.1% | (many diffs) |
| 6 | human | 22944 | KIN | Kin17 DNA and RNA binding p... | XR_930485.2 | 69% | (many diffs) | |
| 7 | human | 22944 | KIN | Kin17 DNA and RNA binding p... | NR_045609.2 | 29.3% | (many diffs) | |
| 8 | mouse | 16588 | Kin | Kin17 DNA and RNA binding p... | NM_025280.2 | 86.1% | 3.4% | (many diffs) |
| 9 | mouse | 16588 | Kin | Kin17 DNA and RNA binding p... | XM_006497365.3 | 80.4% | 3.8% | (many diffs) |
| 10 | mouse | 16588 | Kin | Kin17 DNA and RNA binding p... | XM_006497366.3 | 74.3% | 6.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 294
- ORF length:
- 228
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg aagtcggatt ttcttactcc caaggctatc gccaacagga tcaagtccaa 121 ggggctgcag aagctacgct ggtattgcca gatgtgccag aagcagtgcc gggacgagaa 181 tggctttaag tgtcattgta tgtccgaatc tcatcagaga caactattgc tggcttcaga 241 aaatcctcag cagtttatgg attatttttc agaggaattc cgaaatgact ttctagaact 301 tctcaggaga cgctttggca ctaaaagggt ccacaacaac attgtctaca acgaatacat 361 cagccaccga gagcacatcc acatgaatgc cactcagtgg gaaactctga ctgattttac 421 taagtggctg ggcagagaag gcttgtgcaa agtggacgag acaccaaaag gctggtatat 481 tcagtacata gacagggacc cagaaactat ccgccggcaa ctggaactgg agaaaaagaa 541 aaagcaggac cttgatgatg aagaaaaaac tgccaaattt attgaagagc aagtgagaag 601 aggcctggaa gggaaggaac aggaggtccc tacttttacg gaattaagca gagaaaatga 661 tgaagagaaa gtcacgttta atttgagtaa aggagcatgt agctcatccg gagcaacatc 721 ttccaagtca agtactctgg gaccgagtgc actgaagacg ataggaagtt cagcatcagt 781 gaaacgaaaa gaatcttccc agagctcaac tcagtctaaa gaaaagaaga aaaagaaatc 841 tgcactggat gaaatcatgg agattgaaga ggaaaagaaa agaactgccc gaacagacta 901 ctggctacag cctgaaatta ttgtgaaaat tataaccaag aaactgggag agaaatatca 961 taagaaaaag gctattgtta aggaagtaat tgacaaatat acagctgttg tgaagatgat 1021 tgattctgga gacaagctga aacttgacca gactcattta gagacagtaa ttccagcacc 1081 aggaaaaaga attctagttt taaatggagg ctacagagga aatgaaggta ccctagaatc 1141 catcaatgag aagacttttt cagctactat cgtcattgaa actggccctt taaaaggacg 1201 cagagttgaa ggaattcaat atgaagacat ttctaaactt gcctgcccaa ctttcttgta 1261 caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg 1321 agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta