Construct: ORF ccsbBroad304_14097

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008042.1_s304c1, BRDN0000403389
Derived from:
ccsbBroadEn_14097
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MTHFD1L (25902)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14097
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25902 MTHFD1L methylenetetrahydrofolate d... NM_001350490.1 73.4% 73.2% 1_1delAins289;214A>G;372C>G
2 human 25902 MTHFD1L methylenetetrahydrofolate d... NM_001350487.1 42% 42% 1_1506del;2008A>G;2166C>G
3 human 25902 MTHFD1L methylenetetrahydrofolate d... NM_001350492.1 42% 42% 1_1509del;2011A>G;2169C>G
4 human 25902 MTHFD1L methylenetetrahydrofolate d... NM_001350493.1 42% 42% 1_1509del;2011A>G;2169C>G
5 human 25902 MTHFD1L methylenetetrahydrofolate d... NM_001350486.1 40% 40% 1_1638del;2140A>G;2298C>G
6 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_011535734.2 40% 38.1% (many diffs)
7 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_017010705.1 40% 38.1% (many diffs)
8 human 25902 MTHFD1L methylenetetrahydrofolate d... NM_001242768.1 40% 39.9% 1_1641del;2143A>G;2301C>G
9 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_011535732.2 40% 38.1% (many diffs)
10 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_011535733.2 40% 38.1% (many diffs)
11 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_024446395.1 38.7% 38.7% 1_1731del;2233A>G;2391C>G
12 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_011535731.2 38.1% 36.3% (many diffs)
13 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_011535730.2 38.1% 36.3% (many diffs)
14 human 25902 MTHFD1L methylenetetrahydrofolate d... NM_015440.4 37.3% 37.3% 1_1836del;2338A>G;2496C>G
15 human 25902 MTHFD1L methylenetetrahydrofolate d... NM_001242767.1 37.3% 37.2% 1_1839del;2341A>G;2499C>G
16 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_017010702.2 37.3% 37.2% 1_1839del;2341A>G;2499C>G
17 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_017010703.2 37.3% 37.2% 1_1839del;2341A>G;2499C>G
18 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_005266907.5 35.6% 33.9% (many diffs)
19 human 25902 MTHFD1L methylenetetrahydrofolate d... XM_011535729.3 34.4% 34.4% (many diffs)
20 human 25902 MTHFD1L methylenetetrahydrofolate d... XR_001743322.2 31.1% (many diffs)
21 human 25902 MTHFD1L methylenetetrahydrofolate d... NR_146719.1 30.6% (many diffs)
22 human 25902 MTHFD1L methylenetetrahydrofolate d... XR_002956274.1 30.4% (many diffs)
23 human 101929583 LOC101929583 methylenetetrahydrofolate d... NR_136299.1 28.8% (many diffs)
24 human 25902 MTHFD1L methylenetetrahydrofolate d... NR_146720.1 24.9% (many diffs)
25 mouse 270685 Mthfd1l methylenetetrahydrofolate d... NM_001170785.1 32.7% 35.9% (many diffs)
26 mouse 270685 Mthfd1l methylenetetrahydrofolate d... NM_001170786.1 32.7% 35.9% (many diffs)
27 mouse 270685 Mthfd1l methylenetetrahydrofolate d... NM_172308.4 32.7% 35.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1164
ORF length:
1098
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggtgctggcc ctgacggaca gcctcgcaga catgaaggca cggctgggaa
121ggatggtggt ggccagtgac aaaagcgggc agcctgtgac agcagatgat ttgggggtga
181caggtgcttt gacagttttg atgaaagatg caataaaacc aaacctgatg cagaccctgg
241aagggacacc tgtgttcgtg catgcgggcc cttttgctaa cattgctcac ggcaactctt
301cagtgttggc tgataaaatt gccctgaaac tggttggtga agaaggattt gtagtgaccg
361aagctggctt tggtgctgac atcggaatgg agaaattctt caacatcaag tgccgagctt
421ccggcttggt gcccaacgtg gttgtgttag tggcaacggt gcgagctctg aagatgcatg
481gaggcgggcc aagtgtaacg gctggtgttc ctcttaagaa agaatataca gaggagaaca
541tccagctggt ggcagacggc tgctgtgacc tccagaagca aattcagatc actcagctct
601ttggggttcc cgttgtggtg gctctgaatg tcttcaagac cgacacccgc gctgagattg
661acttggtgtg tgagcttgca aagcgggctg gtgcctttga tgcagtcccc tgctatcact
721ggtcggttgg tggaaaagga tcggtggact tggctcgggc tgtgagagag gctgcgagta
781aaagaagccg attccagttc ctgtatgatg ttcaggttcc aattgtggac aagataagga
841ccattgctca ggctgtctat ggagccaaag atattgaact ctctcctgag gcacaagcca
901aaatagatcg ttacactcaa cagggttttg gaaatttgcc catctgcatg gcaaagaccc
961acctttctct atctcaccaa cctgacaaaa aaggtgtgcc aagggacttc atcttaccta
1021tcagtgacgt ccgggccagc ataggcgctg ggttcattta ccctttggtc ggaacgatga
1081gcaccatgcc aggactgccc acccggccct gcttttatga catagatctt gataccgaaa
1141cagaacaagt taaaggcttg ttctacccaa ctttcttgta caaagtggtt ggtaagccta
1201tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1261gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)