Transcript: Human XR_001743322.2

PREDICTED: Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (MTHFD1L), transcript variant X13, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MTHFD1L (25902)
Length:
3148
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001743322.2
NBCI Gene record:
MTHFD1L (25902)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001743322.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000045399 GCTCTGTATAATCGGCTGGTT pLKO.1 1691 3UTR 100% 2.640 2.112 N MTHFD1L n/a
2 TRCN0000229787 AGCAGTGAAGCCGAGATTATA pLKO_005 542 3UTR 100% 15.000 10.500 N MTHFD1L n/a
3 TRCN0000217958 GTCTGAACATCACTCACATTT pLKO_005 507 3UTR 100% 13.200 9.240 N MTHFD1L n/a
4 TRCN0000045402 CAAGTGACATTGAGATTTCAA pLKO.1 1221 3UTR 100% 5.625 3.938 N MTHFD1L n/a
5 TRCN0000045401 CGATTCCAGTTCCTGTATGAT pLKO.1 2861 3UTR 100% 5.625 3.938 N MTHFD1L n/a
6 TRCN0000045398 GCAGAGAGTATTGGATACAAA pLKO.1 1861 3UTR 100% 5.625 3.938 N MTHFD1L n/a
7 TRCN0000045400 GCCAAAGCTGTAATTGAACTT pLKO.1 749 3UTR 100% 4.950 3.465 N MTHFD1L n/a
8 TRCN0000229788 GTGAGAGAGGCTGCGAGTAAA pLKO_005 2834 3UTR 100% 13.200 7.920 N MTHFD1L n/a
9 TRCN0000036591 GCTGTCTATGGAGCCAAAGAT pLKO.1 2924 3UTR 100% 5.625 2.813 Y LOC389737 n/a
10 TRCN0000036590 GTGGACAAGATAAGGACCATT pLKO.1 2897 3UTR 100% 4.950 2.475 Y LOC389737 n/a
11 TRCN0000141858 GCAGTGATGATGGAGAAGGAA pLKO.1 2277 3UTR 100% 3.000 1.500 Y KIAA1211L n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001743322.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11782 pDONR223 100% 65.2% None (many diffs) n/a
2 ccsbBroad304_11782 pLX_304 0% 65.2% V5 (many diffs) n/a
3 TRCN0000472684 TAAATGCATCCAACAAAAACATAA pLX_317 20.9% 65.2% V5 (many diffs) n/a
4 ccsbBroadEn_14097 pDONR223 100% 31.1% None (many diffs) n/a
5 ccsbBroad304_14097 pLX_304 0% 31.1% V5 (many diffs) n/a
6 TRCN0000481112 TAATGATCTATAGGGTACTATCCT pLX_317 43.6% 31.1% V5 (many diffs) n/a
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