Transcript: Mouse NM_001170786.1

Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Mthfd1l (270685)
Length:
3623
CDS:
181..3114

Additional Resources:

NCBI RefSeq record:
NM_001170786.1
NBCI Gene record:
Mthfd1l (270685)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001170786.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000075944 GCTCTCTACAATCGACTGGTT pLKO.1 1726 CDS 100% 2.640 3.696 N Mthfd1l n/a
2 TRCN0000075946 CCTTGCCAAGGAGATAGGATT pLKO.1 1305 CDS 100% 4.950 3.465 N Mthfd1l n/a
3 TRCN0000075945 GCAACAAAGTTCTCAATGCTT pLKO.1 674 CDS 100% 3.000 2.100 N Mthfd1l n/a
4 TRCN0000075947 GCGCTGAAATTGGTCGGCGAA pLKO.1 2269 CDS 100% 0.720 0.504 N Mthfd1l n/a
5 TRCN0000075943 CGCTTCCTCATACAAGATTAA pLKO.1 3253 3UTR 100% 13.200 7.920 N Mthfd1l n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001170786.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11782 pDONR223 100% 64.5% 69.6% None (many diffs) n/a
2 ccsbBroad304_11782 pLX_304 0% 64.5% 69.6% V5 (many diffs) n/a
3 TRCN0000472684 TAAATGCATCCAACAAAAACATAA pLX_317 20.9% 64.5% 69.6% V5 (many diffs) n/a
4 ccsbBroadEn_14097 pDONR223 100% 32.7% 35.9% None (many diffs) n/a
5 ccsbBroad304_14097 pLX_304 0% 32.7% 35.9% V5 (many diffs) n/a
6 TRCN0000481112 TAATGATCTATAGGGTACTATCCT pLX_317 43.6% 32.7% 35.9% V5 (many diffs) n/a
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