Construct: ORF ccsbBroad304_14279
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010456.1_s304c1, BRDN0000411073
- Derived from:
- ccsbBroadEn_14279
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXL18 (80028)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14279
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_001363442.2 | 53.1% | 53% | 1090C>T;1091_1092insT;1094_2052delinsC |
| 2 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_001363441.2 | 52.3% | 52.2% | 1090C>T;1091_1092insT;1094_2085delinsC |
| 3 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_024963.6 | 50.6% | 50.5% | 1090C>T;1091_1092insT;1094_2154delinsC |
| 4 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_001321213.2 | 45.4% | 45.3% | 1090C>T;1091_1092insT;1094_2403delinsC |
| 5 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_001367780.1 | 36.7% | 36.6% | (many diffs) |
| 6 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_001367781.1 | 36.7% | 36.6% | (many diffs) |
| 7 | mouse | 231863 | Fbxl18 | F-box and leucine-rich repe... | XM_006504694.3 | 57.1% | 60.7% | (many diffs) |
| 8 | mouse | 231863 | Fbxl18 | F-box and leucine-rich repe... | NM_001033312.3 | 42.2% | 44.8% | (many diffs) |
| 9 | mouse | 231863 | Fbxl18 | F-box and leucine-rich repe... | XM_006504693.3 | 39.8% | 42.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1161
- ORF length:
- 1095
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cagctccgga gaggacatat ccaatgatga tgatgacatg caccctgcag 121 cagccgggat ggcagacggg gtccacctcc tagggttctc tgatgagatc ctccttcaca 181 tcctgagtca cgtccccagc acagatctga ttctgaacgt ccggcgtacc tgtcggaagc 241 ttgcagccct gtgccttgac aagagcctca tccacaccgt gttgctgcaa aaggactatc 301 aggcgagcga ggacaaagtg aggcagctgg tgaaggagat cggccgggag atccagcagc 361 tgagcatggc tggctgctac tggctgcctg gctccaccgt ggaacacgtg gcccgctgcc 421 gcagcctggt gaaggtgaac ctctcgggct gccacctcac ttccctgcgc ctctccaaga 481 tgctctcggc cctgcagcac ctgcgctcgc tggccatcga cgtgagcccc ggcttcgacg 541 ccagccagct gagcagcgag tgcaaggcca ccctgagccg cgtgcgggag ctcaagcaga 601 cgctgttcac tccctcctac ggcgtggtgc cctgctgcac cagcctagag aagctgctgc 661 tctacttcga gattctggac cgcacgcgcg agggcgccat cctctcgggc cagcttatgg 721 tgggccagag caacgtgccg cactaccaga acctgcgggt cttctatgcg cgcctggccc 781 ccggctacat caaccaggag gtggtgcggc tctacctggc tgtgcttagc gaccgcactc 841 ctcagaacct ccacgccttc ctcatctccg tccctggcag cttcgcggag agcggcgcca 901 ccaagaacct cctggactcc atggcgcgca atgtcgtgct ggatgccctg cagctgccca 961 agtcctggct gaacggctct tccctcctgc agcacatgaa attcaacaac ccgttctact 1021 tcagtttcag ccgctgtacc ctgtcaggcg gccatctgat ccagcaggtc atcaacggcg 1081 ggaaggacct gcggagcctg gccagcttga acctcagcgg ctgcgtccac tgcctgtccc 1141 cagactcgct gctctgtcac tgcccaactt tcttgtacaa agtggttggt aagcctatcc 1201 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1261 ttaagtcgac aatcaacctc tggatta