Construct: ORF ccsbBroad304_14348

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001479.1_s304c1, BRDN0000405968
Derived from:
ccsbBroadEn_14348
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EGLN2 (112398)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14348
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 112398 EGLN2 egl-9 family hypoxia induci... NM_053046.4 30.6% 30.4% 1_846del;856T>G
2 human 112398 EGLN2 egl-9 family hypoxia induci... NM_080732.4 30.6% 30.4% 1_846del;856T>G
3 human 100529264 RAB4B-EGLN2 RAB4B-EGLN2 readthrough (NM... NR_037791.1 13.1% 1_1894del;1904T>G;2270_2849del
4 mouse 112406 Egln2 egl-9 family hypoxia-induci... XM_017321929.1 39.5% 40.9% (many diffs)
5 mouse 112406 Egln2 egl-9 family hypoxia-induci... NM_053208.4 28.1% 29.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
441
ORF length:
375
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt ggcgggttac ccaggcaacg ggctcgggta cgtaaggcac gttgacaatc
121cccacggcga tgggcgctgc atcacctgta tctattacct gaatcagaac tgggacgtta
181aggtgcatgg cggcctgctg cagatcttcc ctgagggccg gcccgtggta gccaacatcg
241agccactctt tgaccggttg ctcattttct ggtctgaccg gcggaacccc cacgaggtga
301agccagccta tgccaccagg tacgccatca ctgtctggta ttttgatgcc aaggagcggg
361cagcagccaa agacaagtat cagctagcat caggacagaa aggtgtccaa gtacctgtat
421cacagccgcc tacgcccacc tacccaactt tcttgtacaa agtggttggt aagcctatcc
481ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
541ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)