Transcript: Human NR_037791.1

Homo sapiens RAB4B-EGLN2 readthrough (NMD candidate) (RAB4B-EGLN2), long non-coding RNA.

Source:
NCBI, updated 2018-09-16
Taxon:
Homo sapiens (human)
Gene:
RAB4B-EGLN2 (100529264)
Length:
2849
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_037791.1
NBCI Gene record:
RAB4B-EGLN2 (100529264)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_037791.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000022324 CGCATGGCAGACAGCTTAAAT pLKO.1 2289 3UTR 100% 15.000 7.500 Y EGLN2 n/a
2 TRCN0000349633 CGCATGGCAGACAGCTTAAAT pLKO_005 2289 3UTR 100% 15.000 7.500 Y EGLN2 n/a
3 TRCN0000022325 GCTGCATCACCTGTATCTATT pLKO.1 1965 3UTR 100% 13.200 6.600 Y EGLN2 n/a
4 TRCN0000318672 GCTGCATCACCTGTATCTATT pLKO_005 1965 3UTR 100% 13.200 6.600 Y EGLN2 n/a
5 TRCN0000381738 GGTCAGTGACGCGGAGTTATT pLKO_005 369 3UTR 100% 13.200 6.600 Y RAB4B n/a
6 TRCN0000379712 GACTGTGAAGCTACAGATTTG pLKO_005 322 3UTR 100% 10.800 5.400 Y RAB4B n/a
7 TRCN0000380533 GCACTATCCTCAACAAGATTG pLKO_005 657 3UTR 100% 10.800 5.400 Y RAB4B n/a
8 TRCN0000381336 GCAGTGCAGGAACTGGCAAAT pLKO_005 201 3UTR 100% 10.800 5.400 Y RAB4B n/a
9 TRCN0000380756 TCATTGAGAATAAGTTCAAAC pLKO_005 240 3UTR 100% 10.800 5.400 Y RAB4B n/a
10 TRCN0000073024 CGCACTATCCTCAACAAGATT pLKO.1 656 3UTR 100% 5.625 2.813 Y RAB4B n/a
11 TRCN0000380881 GATGGGCTCTGGCATTCAGTA pLKO_005 703 3UTR 100% 4.950 2.475 Y RAB4B n/a
12 TRCN0000009747 GCCAACATCGAGCCACTCTTT pLKO.1 2060 3UTR 100% 4.950 2.475 Y Egln2 n/a
13 TRCN0000273754 GCCAACATCGAGCCACTCTTT pLKO_005 2060 3UTR 100% 4.950 2.475 Y Egln2 n/a
14 TRCN0000380038 GGTCATCCTCTGTGGCAACAA pLKO_005 502 3UTR 100% 4.950 2.475 Y RAB4B n/a
15 TRCN0000022327 ACTGGGACGTTAAGGTGCATG pLKO.1 1998 3UTR 100% 4.050 2.025 Y EGLN2 n/a
16 TRCN0000349632 ACTGGGACGTTAAGGTGCATG pLKO_005 1998 3UTR 100% 4.050 2.025 Y EGLN2 n/a
17 TRCN0000380099 AGATTGACTCAGGCGAGCTAG pLKO_005 672 3UTR 100% 4.050 2.025 Y RAB4B n/a
18 TRCN0000381059 CTCAAGTGTGCCCGCACTATC pLKO_005 644 3UTR 100% 3.600 1.800 Y RAB4B n/a
19 TRCN0000073026 ACTGGCAAATCATGTCTCCTT pLKO.1 212 3UTR 100% 2.640 1.320 Y RAB4B n/a
20 TRCN0000022326 GCCACTCTTTGACCGGTTGCT pLKO.1 2071 3UTR 100% 0.880 0.440 Y EGLN2 n/a
21 TRCN0000318601 GCCACTCTTTGACCGGTTGCT pLKO_005 2071 3UTR 100% 0.880 0.440 Y EGLN2 n/a
22 TRCN0000022328 CTGGGACGTTAAGGTGCATGG pLKO.1 1999 3UTR 100% 0.750 0.375 Y EGLN2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_037791.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14348 pDONR223 100% 13.1% None 1_1894del;1904T>G;2270_2849del n/a
2 ccsbBroad304_14348 pLX_304 0% 13.1% V5 1_1894del;1904T>G;2270_2849del n/a
3 TRCN0000472254 GTTTATTACAACGAGACATCGATC pLX_317 90.6% 13.1% V5 1_1894del;1904T>G;2270_2849del n/a
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