Construct: ORF ccsbBroad304_14502

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF017732.1_s304c1, BRDN0000461082
• Derived from: ccsbBroadEn_14502
• DNA Barcode: None
• Epitope Tag: V5 (not translated due to frame shift)
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: MGC70870 (403340)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_14502
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	1488	CTBP2	C-terminal binding protein 2	XM_011539357.2	31.2%	27.5%	(many diffs)
2	human	1488	CTBP2	C-terminal binding protein 2	XM_011539358.2	31.2%	27.5%	(many diffs)
3	human	1488	CTBP2	C-terminal binding protein 2	XM_024447831.1	31.2%	27.5%	(many diffs)
4	human	1488	CTBP2	C-terminal binding protein 2	NM_001083914.3	29.5%	26%	(many diffs)
5	human	1488	CTBP2	C-terminal binding protein 2	NM_001290214.3	29.5%	26%	(many diffs)
6	human	1488	CTBP2	C-terminal binding protein 2	NM_001290215.3	29.5%	26%	(many diffs)
7	human	1488	CTBP2	C-terminal binding protein 2	NM_001321012.2	29.5%	26%	(many diffs)
8	human	1488	CTBP2	C-terminal binding protein 2	NM_001321013.2	29.5%	26%	(many diffs)
9	human	1488	CTBP2	C-terminal binding protein 2	NM_001321014.2	29.5%	26%	(many diffs)
10	human	1488	CTBP2	C-terminal binding protein 2	NM_001329.4	29.5%	26%	(many diffs)
11	human	1488	CTBP2	C-terminal binding protein 2	XM_005269561.3	29.5%	26%	(many diffs)
12	human	1488	CTBP2	C-terminal binding protein 2	XM_005269564.3	29.5%	26%	(many diffs)
13	human	1488	CTBP2	C-terminal binding protein 2	XM_005269567.3	29.5%	26%	(many diffs)
14	human	1488	CTBP2	C-terminal binding protein 2	XM_005269568.5	29.5%	26%	(many diffs)
15	human	1488	CTBP2	C-terminal binding protein 2	XM_005269569.3	29.5%	26%	(many diffs)
16	human	1488	CTBP2	C-terminal binding protein 2	XM_005269571.3	29.5%	26%	(many diffs)
17	human	1488	CTBP2	C-terminal binding protein 2	XM_005269572.4	29.5%	26%	(many diffs)
18	human	1488	CTBP2	C-terminal binding protein 2	XM_006717642.3	29.5%	26%	(many diffs)
19	human	1488	CTBP2	C-terminal binding protein 2	XM_011539351.2	29.5%	26%	(many diffs)
20	human	1488	CTBP2	C-terminal binding protein 2	XM_011539353.2	29.5%	26%	(many diffs)
21	human	1488	CTBP2	C-terminal binding protein 2	XM_011539354.2	29.5%	26%	(many diffs)
22	human	1488	CTBP2	C-terminal binding protein 2	XM_011539355.2	29.5%	26%	(many diffs)
23	human	1488	CTBP2	C-terminal binding protein 2	XM_017015756.2	29.5%	26%	(many diffs)
24	human	1488	CTBP2	C-terminal binding protein 2	XM_017015757.2	29.5%	26%	(many diffs)
25	human	1488	CTBP2	C-terminal binding protein 2	XM_024447830.1	29.5%	26%	(many diffs)
26	human	1488	CTBP2	C-terminal binding protein 2	NM_001363508.2	25.6%	22.5%	(many diffs)
27	human	403340	MGC70870	C-terminal binding protein ...	NR_003682.1	13.9%		(many diffs)
28	human	1488	CTBP2	C-terminal binding protein 2	NM_022802.3	13.3%	11.7%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

69

ORF end:

516

ORF length:

447

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcaccat gcaggagatc cacgagaaag ttctaaacga agtcgtgggc gccatgatgt
121	accacaactt ctccctcacc agggaggacc tggaaaattt caaggccctg agagtgatca
181	tgcaggtggg cagtggctac gacaacgtgg ccatcaaggc tgctggcgag ctcgaaattg
241	ctctgtgcag catcccgtcc gcagccgtgg aagagacagc cgactccacc accggccaca
301	tcctcaatct gtactggggg aacaggtcac tgtaccaggc actgagggaa ggcacacgag
361	ttcacagcgt ggagcagatt ggcgaggtgg cctcagtaaa ggcccgcatt cgtggggaga
421	tattgggcct catcggcttc ggtcgcacgc agcaggaggt tgcagttaga gccaaggcct
481	ttgcaggatg ggatcgagcg gtccctggtg tgcattgcca actttcttgt acaaagtggt
541	tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gagctagcgc
601	taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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