Transcript: Human XM_011539358.2

PREDICTED: Homo sapiens C-terminal binding protein 2 (CTBP2), transcript variant X18, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CTBP2 (1488)
Length:
3070
CDS:
606..1868

Additional Resources:

NCBI RefSeq record:
XM_011539358.2
NBCI Gene record:
CTBP2 (1488)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011539358.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000273908 ATCAATGACTTTACCATAAAG pLKO_005 1287 CDS 100% 13.200 9.240 N CTBP2 n/a
2 TRCN0000285087 ATCAGCGCCTTGGTCAGTAAT pLKO_005 1622 CDS 100% 13.200 9.240 N CTBP2 n/a
3 TRCN0000109338 CCTTTGGATTCAGCGTCATAT pLKO.1 1132 CDS 100% 13.200 9.240 N Ctbp2 n/a
4 TRCN0000287705 CCTTTGGATTCAGCGTCATAT pLKO_005 1132 CDS 100% 13.200 9.240 N Ctbp2 n/a
5 TRCN0000273846 TCGCATCCCAGAAAGCTTAAG pLKO_005 1571 CDS 100% 10.800 7.560 N CTBP2 n/a
6 TRCN0000013743 CGCATAGGATTGAAGACAGTA pLKO.1 2375 3UTR 100% 4.950 3.465 N CTBP2 n/a
7 TRCN0000013744 GCCTTTGGATTCAGCGTCATA pLKO.1 1131 CDS 100% 4.950 3.465 N CTBP2 n/a
8 TRCN0000273907 GCCTTTGGATTCAGCGTCATA pLKO_005 1131 CDS 100% 4.950 3.465 N CTBP2 n/a
9 TRCN0000109339 GCCCTCAAGGAGGGCAGGATA pLKO.1 1380 CDS 100% 0.000 0.000 N Ctbp2 n/a
10 TRCN0000433738 ACCACCTCATCAATGACTTTA pLKO_005 1279 CDS 100% 13.200 7.920 N Ctbp1 n/a
11 TRCN0000273847 TGCTTACGGAAGCGCTGAAAG pLKO_005 2040 3UTR 100% 10.800 6.480 N CTBP2 n/a
12 TRCN0000013745 CACTGCAATCTCAACGAACAT pLKO.1 1254 CDS 100% 4.950 2.970 N CTBP2 n/a
13 TRCN0000013746 CCTGAGAGTGATCGTGCGGAT pLKO.1 821 CDS 100% 0.720 0.432 N CTBP2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011539358.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15391 pDONR223 0% 94.3% 94.3% None 0_1ins75 n/a
2 ccsbBroad304_15391 pLX_304 0% 94.3% 94.3% V5 0_1ins75 n/a
3 TRCN0000478688 GCTGGCTACGTTCAGGCTCCGTCT pLX_317 27.8% 94.3% 94.3% V5 0_1ins75 n/a
4 ccsbBroadEn_10758 pDONR223 100% 81.8% 81.6% None 0_1ins279;799G>A n/a
5 ccsbBroad304_10758 pLX_304 0% 81.8% 81.6% V5 0_1ins279;799G>A n/a
6 TRCN0000469969 TATCAGCAGTAACGCAGCTATGGG pLX_317 28.2% 81.8% 81.6% V5 0_1ins279;799G>A n/a
7 ccsbBroadEn_14502 pDONR223 100% 31.2% 27.5% None (many diffs) n/a
8 ccsbBroad304_14502 pLX_304 0% 31.2% 27.5% V5 (not translated due to frame shift) (many diffs) n/a
9 TRCN0000476958 AGGCAGAAGCTAACATTTTCTCGG pLX_317 93.1% 31.2% 27.5% V5 (not translated due to frame shift) (many diffs) n/a
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