Transcript: Human NR_003682.1

Homo sapiens C-terminal binding protein 2 pseudogene (MGC70870), non-coding RNA.

Source:
NCBI, updated 2018-05-24
Taxon:
Homo sapiens (human)
Gene:
MGC70870 (403340)
Length:
3126
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_003682.1
NBCI Gene record:
MGC70870 (403340)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_003682.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000016445 ACGCAGCAGGAGGTTGCAGTT pLKO.1 1126 3UTR 100% 1.350 0.945 N MGC70870 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_003682.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10758 pDONR223 100% 39.8% None (many diffs) n/a
2 ccsbBroad304_10758 pLX_304 0% 39.8% V5 (many diffs) n/a
3 TRCN0000469969 TATCAGCAGTAACGCAGCTATGGG pLX_317 28.2% 39.8% V5 (many diffs) n/a
4 ccsbBroadEn_15391 pDONR223 0% 37% None (many diffs) n/a
5 ccsbBroad304_15391 pLX_304 0% 37% V5 (many diffs) n/a
6 TRCN0000478688 GCTGGCTACGTTCAGGCTCCGTCT pLX_317 27.8% 37% V5 (many diffs) n/a
7 ccsbBroadEn_14502 pDONR223 100% 13.9% None (many diffs) n/a
8 ccsbBroad304_14502 pLX_304 0% 13.9% V5 (not translated due to frame shift) (many diffs) n/a
9 TRCN0000476958 AGGCAGAAGCTAACATTTTCTCGG pLX_317 93.1% 13.9% V5 (not translated due to frame shift) (many diffs) n/a
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