Transcript: Human NM_001321014.2

Homo sapiens C-terminal binding protein 2 (CTBP2), transcript variant 8, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
CTBP2 (1488)
Length:
6884
CDS:
346..1683

Additional Resources:

NCBI RefSeq record:
NM_001321014.2
NBCI Gene record:
CTBP2 (1488)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321014.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000273908 ATCAATGACTTTACCATAAAG pLKO_005 1102 CDS 100% 13.200 9.240 N CTBP2 n/a
2 TRCN0000285087 ATCAGCGCCTTGGTCAGTAAT pLKO_005 1437 CDS 100% 13.200 9.240 N CTBP2 n/a
3 TRCN0000109338 CCTTTGGATTCAGCGTCATAT pLKO.1 947 CDS 100% 13.200 9.240 N Ctbp2 n/a
4 TRCN0000287705 CCTTTGGATTCAGCGTCATAT pLKO_005 947 CDS 100% 13.200 9.240 N Ctbp2 n/a
5 TRCN0000273846 TCGCATCCCAGAAAGCTTAAG pLKO_005 1386 CDS 100% 10.800 7.560 N CTBP2 n/a
6 TRCN0000013743 CGCATAGGATTGAAGACAGTA pLKO.1 2190 3UTR 100% 4.950 3.465 N CTBP2 n/a
7 TRCN0000013744 GCCTTTGGATTCAGCGTCATA pLKO.1 946 CDS 100% 4.950 3.465 N CTBP2 n/a
8 TRCN0000273907 GCCTTTGGATTCAGCGTCATA pLKO_005 946 CDS 100% 4.950 3.465 N CTBP2 n/a
9 TRCN0000013747 TGGACAGAATTTGTGAAGGTA pLKO.1 386 CDS 100% 3.000 2.100 N CTBP2 n/a
10 TRCN0000109339 GCCCTCAAGGAGGGCAGGATA pLKO.1 1195 CDS 100% 0.000 0.000 N Ctbp2 n/a
11 TRCN0000433738 ACCACCTCATCAATGACTTTA pLKO_005 1094 CDS 100% 13.200 7.920 N Ctbp1 n/a
12 TRCN0000273847 TGCTTACGGAAGCGCTGAAAG pLKO_005 1855 3UTR 100% 10.800 6.480 N CTBP2 n/a
13 TRCN0000013745 CACTGCAATCTCAACGAACAT pLKO.1 1069 CDS 100% 4.950 2.970 N CTBP2 n/a
14 TRCN0000013746 CCTGAGAGTGATCGTGCGGAT pLKO.1 636 CDS 100% 0.720 0.432 N CTBP2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321014.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15391 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15391 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000478688 GCTGGCTACGTTCAGGCTCCGTCT pLX_317 27.8% 100% 100% V5 n/a
4 ccsbBroadEn_10758 pDONR223 100% 85.3% 82.2% None (many diffs) n/a
5 ccsbBroad304_10758 pLX_304 0% 85.3% 82.2% V5 (many diffs) n/a
6 TRCN0000469969 TATCAGCAGTAACGCAGCTATGGG pLX_317 28.2% 85.3% 82.2% V5 (many diffs) n/a
7 ccsbBroadEn_14502 pDONR223 100% 29.5% 26% None (many diffs) n/a
8 ccsbBroad304_14502 pLX_304 0% 29.5% 26% V5 (not translated due to frame shift) (many diffs) n/a
9 TRCN0000476958 AGGCAGAAGCTAACATTTTCTCGG pLX_317 93.1% 29.5% 26% V5 (not translated due to frame shift) (many diffs) n/a
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