Construct: ORF ccsbBroad304_14547
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001912.1_s304c1, BRDN0000401948
- Derived from:
- ccsbBroadEn_14547
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BCL6 (604)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14547
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 604 | BCL6 | BCL6 transcription repressor | NM_001130845.2 | 98.8% | 55.2% | (many diffs) |
| 2 | human | 604 | BCL6 | BCL6 transcription repressor | NM_001706.5 | 98.8% | 55.2% | (many diffs) |
| 3 | human | 604 | BCL6 | BCL6 transcription repressor | XM_005247694.4 | 98.8% | 55.2% | (many diffs) |
| 4 | human | 604 | BCL6 | BCL6 transcription repressor | NM_001134738.1 | 90.9% | 59.9% | (many diffs) |
| 5 | human | 604 | BCL6 | BCL6 transcription repressor | XM_011513062.3 | 90.9% | 59.9% | (many diffs) |
| 6 | mouse | 12053 | Bcl6 | B cell leukemia/lymphoma 6 | NM_001348026.1 | 88% | 51.3% | (many diffs) |
| 7 | mouse | 12053 | Bcl6 | B cell leukemia/lymphoma 6 | NM_009744.4 | 88% | 51.3% | (many diffs) |
| 8 | mouse | 12053 | Bcl6 | B cell leukemia/lymphoma 6 | XM_017316855.1 | 88% | 51.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1350
- ORF length:
- 1281
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctcgccg gctgacagct gtatccagtt cacccgccat gccagtgatg 121 ttcttctcaa ccttaatcgt ctccggagtc gagacatctt gactgatgtt gtcattgttg 181 tgagccgtga gcagtttaga gcccataaaa cggtcctcat ggcctgcagt ggcctgttct 241 atagcatctt tacagaccag ttgaaatgca accttagtgt gatcaatcta gatcctgaga 301 tcaaccctga gggattctgc atcctcctgg acttcatgta cacatctcgg ctcaatttgc 361 gggagggcaa catcatggct gtgatggcca cggctatgta cctgcagatg gagcatgttg 421 tggacacttg ccggaagttt attaaggcca gtgaagcaga gatggtttct gccatcaagc 481 ctcctcgtga agagttcctc aacagccgga tgctgatgcc ccaagacatc atggcctatc 541 ggggtcgtga ggtggtggag aacaacctgc cactgaggag cgcccctggg tgtgagagca 601 gagcctttgc ccccagcctg tacagtggcc tgtccgcacc gccagcctct tattccatgt 661 acagccaccT CCCTGTCAGC AGCCTCCTCT TCTCCGATGA GGAGTTTCGG GATGTCCGGA 721 TGCCTGTGGC CAACCCCTTC CCCAAGGAGC GGGCACTCCC ATGTGATAGT GCCAGGCCAG 781 TCCCTGGTGA GTACAGCCGG CCGACTTTGG AGGTGTCCCC CAATGTGTGC CACAGCAATA 841 TCTATTCACC CAAGGAAACA ATCCCAGAAG AGGCACGAAG TGATATGCAC TACAGTGTGG 901 CTGAGGGCCT CAAACCTGCT GCCCCCTCAG CCCGAAATGC CCCCTACTTC CCTTGTGACA 961 AGGCCAGCAA AGAAGAAGAG AGACCCTCCT CGGAAGATGA GATTGCCATG CATTTCGAGC 1021 CCCCCAATGC ACCCCTGAAC CGGAAGGGTC TGGTTAGTCC ACAGAGCCCC CAGAAATCTG 1081 ACTGCCAGCC CAACTCGCCC ACAGAGTCCT GCAGCAGTAA GAATGCCTGC ATCCTCCAGG 1141 CTTCTGGCTC CCCTCCAGCC AAGAGCCCCA CTGACCCCAA AGCCTGCTTG CCAACTTTCT 1201 TGTACtcaac agcctcaacc agaatgccaa accagagggg cctgagcagg ctgagctggg 1261 ccgcctttcc ccacgagcct acacggcccc acctgcctgc cagccaccca tggagcctga 1321 gaaccttgac ctccagtccc caaccaagct gagtgccagc ggggaggact ccaccatccc 1381 acaagccagc cggctcaata acatcgttaa caggtccatg acgggctctc cccgcagcag 1441 cagcgagagc cactcaccac tctacatgca ccccccgaag tgcacgtcct gcggctctca 1501 gtccccacag catgcagaga tgtgcctcca caccgctggc cccacgttcc ctgaggagat 1561 gggagagacc cagtctgagt actcagattc tagctgtgag aacggggcct tcttctgcaa 1621 tgagtgtgac tgccgcttct ctgaggaggc ctcactcaag aggcacacgc tgcagaccca 1681 cagtgacaaa ccctacaagt gtgaccgctg ccaggcctcc ttccgctaca agggcaacct 1741 cgccagccac aagaccgtcc ataccggtga gaaaccctat cgttgcaaca tctgtggggc 1801 ccagttcaac cggccagcca acctgaaaac ccacactcga attcactctg gagagaagcc 1861 ctacaaatgc gaaacctgcg gagccagatt tgtacaggtg gcccacctcc gtgcccatgt 1921 gcttatccac actggtgaga agccctatcc ctgtgaaatc tgtggcaccc gtttccggca 1981 ccttcagact ctgaagagcc acctgcgaat ccacacagga gagaaacctt accattgtga 2041 gaagtgtaac ctgcatttcc gtcacaaaag ccagctgcga cttcacttgc gccagaagca 2101 tggcgccatc accaacacca aggtgcaata ccgcgtgtca gccactgacc tgcctccgga 2161 gctccccaaa gcctgcttgc caactttctt gtacaaagtg gttggtaagc ctatccctaa 2221 ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt ggcgcgttaa 2281 gtcgacaatc aacctctgga tta