Construct: ORF ccsbBroad304_14609

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018916.1_s304c1, BRDN0000459400
Derived from:
ccsbBroadEn_14609
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
DAPK1 (1612)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14609
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1612 DAPK1 death associated protein ki... NM_001288729.1 99.3% 15.8% (many diffs)
2 human 1612 DAPK1 death associated protein ki... NM_001288730.2 99.3% 15.8% (many diffs)
3 human 1612 DAPK1 death associated protein ki... NM_001288731.2 99.3% 15.8% (many diffs)
4 human 1612 DAPK1 death associated protein ki... NM_004938.4 99.3% 15.8% (many diffs)
5 human 1612 DAPK1 death associated protein ki... XM_017014360.1 69.9% 5.2% (many diffs)
6 human 1612 DAPK1 death associated protein ki... XM_005251757.4 57.5% 26.8% (many diffs)
7 mouse 69635 Dapk1 death associated protein ki... NM_029653.3 87.1% 15.8% (many diffs)
8 mouse 69635 Dapk1 death associated protein ki... NM_134062.2 87.1% 15.8% (many diffs)
9 mouse 69635 Dapk1 death associated protein ki... NM_001285917.1 86.4% 15.6% (many diffs)
10 mouse 69635 Dapk1 death associated protein ki... XM_006517370.3 86.4% 15.6% (many diffs)
11 mouse 69635 Dapk1 death associated protein ki... XM_006517371.3 85.9% 14.4% (many diffs)
12 mouse 69635 Dapk1 death associated protein ki... XM_017315598.1 85.1% 14.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
774
ORF length:
705
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaccgtgttc aggcaggaaa acgtggatga ttactacgac accggcgagg
121aacttggcag tggacagttt gcggttgtga agaaatgccg tgagaaaagc accggcctcc
181agtatgccgc caaattcatc aagaaaagga ggactaagtc cagccggcgg ggtgtgagcc
241gcgaggacat cgagcgggag gtcagcatcc tgaaggagat ccagcacccc aatgtcatca
301ccctgcacga ggtctatgag aacaagacgg acgtcatcct gatcttggaa ctcgttgcag
361gtggcgagct gtttgacttc ttagctgaaa aggaatcttt aactgaagag gaagcaactg
421aatttctcaa acaaattctt aatggtgttt actacctgca ctcccttcaa atcgcccact
481ttgatcttaa gcctgagaac ataatgcttt tggatagaaa tgtccccaaa cctcggatca
541agatcattga ctttgggttg gcccataaaa ttgactttgg aaatgaattt aaaaacatat
601ttgggactcc agagtttgtc gctcctgaga tagtcaacta tgaacctctt ggtcttgagg
661cagatatgtg gagtatcggg gtaataacct atatcctcct aagtggggcc tccccatttc
721ttggagacac taagcaagaa acgttagcaa tgtatccgct gtcaactacg atttgagatg
781atacttcagt ataccagtgc cctagccaaa gatttcatag agacttctgg tcagatccaa
841agagagatga cattcagata gttgcagcat ccctggatca agcctaaaga tacacaacag
901gcacttagta gaaaagcatc agcagtaaac atggagaaat tcaagaagtt tgcagcccgg
961aaaaaatgga aacaatccgt tcgcttgata tcactgtgcc aaagattatc caggtcattc
1021ctgtccagaa gtaacatgag tgttgccaga agcgatgata ctctggatga ggaagactcc
1081tttgtgatga aagccatcat ccatgccatc aacgatgaca atgtcccagg cctgcagcac
1141cttctgggct cattatccaa ctatgatgtt aaccaaccca acaagcacgg gacacctcca
1201ttactcattg ctgctggctg tgggaatatt caaatactac agttgctcat taaaagaggc
1261tcgagaatcg atgtccagga taagggcggg tccaatgccg tctactgggc tgctcggcat
1321ggccacgtcg ataccttgaa atttctcagt gagaacaaat gccctttgga tgtgaaagac
1381aagtctggag agatggccct ccacgtggca gctcgctatg gccatgctga cgtggctcag
1441ttactgtgca gcttcggctc aaatcccaat atccaggaca aggaagaaga aacccccctg
1501cactgtgctg cttggcacgg ctattactct gtggccaaag ccctttgtga agccggctgt
1561aacgtgaaca tcaagaaccg agaaggagag acgcccctcc tgacagcctc tgccaggggc
1621taccacgaca tcgtggagtg tctggccgaa catggagccg accttaatgc ttgcgacaag
1681gacggacaca ttgcccttca tctggctgta agacggtgtc agatggaggt aatcaagact
1741ctcctcagcc aagggtgttt cgtcgattat caagacaggc acggcaatac tcccctccat
1801gtggcatgta aagatggcaa catgcctatc gtggtggccc tctgtgaagc aaactgcaat
1861ttggacatct ccaacaagta tgggcgaacg cctctgcacc ttgcggccaa caacggaatc
1921ctagacgtgg tccggtatct ctgtctgatg ggagccagcg ttgaggcgct gaccacggac
1981ggaaagacgg cagaagatct tgctagatcg gaacagcacg agcacgtagc aggtctcctt
2041gcaagacttc gaaaggatac gcaccgagga ctcttcatcc agcagctccg acccacacag
2101aacctgcagc caagaattaa gctcaagctg tttggccact cgggatccgg gaaaaccacc
2161cttgtagaat ctctcaagtg tgggctgctg aggagctttt tcagaaggcg tcggcccaga
2221ctgtcttcca ccaactccag caggttccca ccttcacccc tggcttctaa gcccacagtc
2281tcagtgagca tcaacaacct gtacccaggc tgcgagaacg tgagtgtgag gagccgcagc
2341atgatgttcg agccgggtct taccaaaggg atgctggagg tgtttgtggc cccgacccac
2401cacccgcact gctcggccga tgaccagtcc accaaggcca tcgacatcca gaacgcttat
2461ttgaatggag ttggcgattt cagcgtgtgg gagttctctg gaaatcctgt gtatttctgc
2521tgttatgact attttgctgc aaatgatccc acgtcaatcc atgttgttgt ctttagtcta
2581gaagagccct atgagatcca gctgaaccaa gtgattttct ggctcagttt cctgaagtcc
2641cttgtcccag ttgaagaacc catagccttc ggtggcaagc tgaagaaccc actccaagtt
2701gtcctggtgg ccacccacgc tgacatcatg aatgttcctc gaccggctgg aggcgagttt
2761ggatatgaca aagacacatc gttgctgaaa gagattagga acaggtttgg aaatgatctt
2821cacatttcaa ataagctgtt tgttctggat gctggggctt ctgggtcaaa ggacatgaag
2881gtacttcgaa atcatctgca agaaatacga agccagattg tttcggtctg tcctcccatg
2941actcacctgt gtgagaaaat catctccacg ctgccttcct ggaggaagct caatggaccc
3001aaccagctga tgtcgctgca gcagtttgtg tacgacgtgc aggaccagct gaaccccctg
3061gccagcgagg aggacctcag gcgcattgct cagcagctcc acagcacagg cgagatcaac
3121atcatgcaaa gtgaaacagt tcaggacgtg ctgctcctgg acccccgctg gctctgcaca
3181aacgtcctgg ggaagttgct gtccgtggag accccacggg cgctgcacca ctaccggggc
3241cgctacaccg tggaggacat ccagcgcctg gtgcccgaca gcgacgtgga ggagctgctg
3301cagatcctcg atgccatgga catctgcgcc cgggacctga gcagcgggac catggtggac
3361gtcccagccc tgatcaagac agacaacctg caccgctcct gggctgatga ggaggacgag
3421gtgatggtgt atggtggcgt gcgcatcgtg cccgtggaac acctcacccc cttcccatgt
3481ggcatctttc acaaggtcca ggtgaacctg tgccggtgga tccaccagca aagccacaga
3541ggcggacgcg gacatcccgc ttgttggtga atggttgcaa gctgcccaac gtgggggccg
3601agctgctggt gctgctggtc aaacacgccc aaggcattga ggtccaggtc cgcggcctgg
3661agacggagaa gatcaagtgc tgcctgctgc tggactcggt gtgcagcacc attgagaacg
3721tcatggccac cacgctgcca gggctcctga ccgtgaagca ttacctgagc ccccagcagc
3781tgcgggagca ccatgagccc gtcatgatct accagccacg ggacttcttc cgggcacaga
3841ctctgaagga aacctcactg accaacacca tgggggggta caaggaaagc ttcagcagca
3901tcatgtgctt cgggtgtcac gacgtctact cacaggccag cctcggcatg gacatccatg
3961catcagacct gaacctcctc actcggagga aactgagtcg cctgctggac ccgcccgacc
4021ccctggggaa ggactggtgc cttctcgcca tgaacttagg cctccctgac ctcgtggcaa
4081agtacaacac cagtaacggg gctcccaagg atttcctccc cagccccctc cacgccctgc
4141tgcgggaatg gaccacctac cctgagagca cagtgggcac cctcatgtcc aaactgaggg
4201agctgggtcg ccgggatgcc gcagactttt tgctgaaggc atcctctgtg ttcaaaatca
4261acctggatgg caatggccag gaggcctatg cctcgagctg caacagcggc acctcttaca
4321attccattag ctctgttgta tcccggttgc caactttctt gtacaaagtg gttggtaagc
4381ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
4441ggcgcgttaa gtcgacaatc aacctctgga tta

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