Construct: ORF ccsbBroad304_14696

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019003.1_s304c1, BRDN0000459804
Derived from:
ccsbBroadEn_14696
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MET (4233)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14696
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4233 MET MET proto-oncogene, recepto... NM_000245.4 100% 100%
2 human 4233 MET MET proto-oncogene, recepto... NM_001127500.3 98.7% 98.7% 2263_2316del
3 human 4233 MET MET proto-oncogene, recepto... XM_011516223.1 98.6% 98.6% 1_57del
4 human 4233 MET MET proto-oncogene, recepto... NM_001324402.2 69% 69% 0_1ins1290
5 human 4233 MET MET proto-oncogene, recepto... XM_006715990.2 69% 69% 0_1ins1290
6 human 4233 MET MET proto-oncogene, recepto... NM_001324401.2 66.6% 65.9% (many diffs)
7 human 4233 MET MET proto-oncogene, recepto... XR_001744772.1 61% 1_231del;2494_2495ins100;4302_6566del
8 mouse 17295 Met met proto-oncogene NM_008591.2 85.7% 88.9% (many diffs)
9 mouse 17295 Met met proto-oncogene XM_011241042.2 85.7% 88.9% (many diffs)
10 mouse 17295 Met met proto-oncogene XM_011241043.2 85.7% 88.9% (many diffs)
11 mouse 17295 Met met proto-oncogene XM_006505002.3 82.5% 85.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4239
ORF length:
4170
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaaggccccc gctgtgcttg cacctggcat cctcgtgctc ctgtttacct
121tggtgcagag gagcaatggg gagtgtaaag aggcactagc aaagtccgag atgaatgtga
181atatgaagta tcagcttccc aacttcaccg cggaaacacc catccagaat gtcattctac
241atgagcatca cattttcctt ggtgccacta actacattta tgttttaaat gaggaagacc
301ttcagaaggt tgctgagtac aagactgggc ctgtgctgga acacccagat tgtttcccat
361gtcaggactg cagcagcaaa gccaatttat caggaggtgt ttggaaagat aacatcaaca
421tggctctagt tgtcgacacc tactatgatg atcaactcat tagctgtggc agcgtcaaca
481gagggacctg ccagcgacat gtctttcccc acaatcatac tgctgacata cagtcggagg
541ttcactgcat attctcccca cagatagaag agcccagcca gtgtcctgac tgtgtggtga
601gcgccctggg agccaaagtc ctttcatctg taaaggaccg gttcatcaac ttctttgtag
661gcaataccat aaattcttct tatttcccag atcatccatt gcattcgata tcagtgagaa
721ggctaaagga aacgaaagat ggttttatgt ttttgacgga ccagtcctac attgatgttt
781tacctgagtt cagagattct taccccatta agtatgtcca tgcctttgaa agcaacaatt
841ttatttactt cttgacggtc caaagggaaa ctctagatgc tcagactttt cacacaagaa
901taatcaggtt ctgttccata aactctggat tgcattccta catggaaatg cctctggagt
961gtattctcac agaaaagaga aaaaagagat ccacaaagaa ggaagtgttt aatatacttc
1021aggctgcgta tgtcagcaag cctggggccc agcttgctag acaaatagga gccagcctga
1081atgatgacat tcttttcggg gtgttcgcac aaagcaagcc agattctgcc gaaccaatgg
1141atcgatctgc catgtgtgca ttccctatca aatatgtcaa cgacttcttc aacaagatcg
1201tcaacaaaaa caatgtgaga tgtctccagc atttttacgg acccaatcat gagcactgct
1261ttaataggac acttctgaga aattcatcag gctgtgaagc gcgccgtgat gaatatcgaa
1321cagagtttac cacagctttg cagcgcgttg acttattcat gggtcaattc agcgaagtcc
1381tcttaacatc tatatccacc ttcattaaag gagacctcac catagctaat cttgggacat
1441cagagggtcg cttcatgcag gttgtggttt ctcgatcagg accatcaacc cctcatgtga
1501attttctcct ggactcccat ccagtgtctc cagaagtgat tgtggagcat acattaaacc
1561aaaatggcta cacactggtt atcactggga agaagatcac gaagatccca ttgaatggct
1621tgggctgcag acatttccag tcctgcagtc aatgcctctc tgccccaccc tttgttcagt
1681gtggctggtg ccacgacaaa tgtgtgcgat cggaggaatg cctgagcggg acatggactc
1741aacagatctg tctgcctgca atctacaagg ttttcccaaa tagtgcaccc cttgaaggag
1801ggacaaggct gaccatatgt ggctgggact ttggatttcg gaggaataat aaatttgatt
1861taaagaaaac tagagttctc cttggaaatg agagctgcac cttgacttta agtgagagca
1921cgatgaatac attgaaatgc acagttggtc ctgccatgaa taagcatttc aatatgtcca
1981taattatttc aaatggccac gggacaacac aatacagtac attctcctat gtggatcctg
2041taataacaag tatttcgccg aaatacggtc ctatggctgg tggcacttta cttactttaa
2101ctggaaatta cctaaacagt gggaattcta gacacatttc aattggtgga aaaacatgta
2161ctttaaaaag tgtgtcaaac agtattcttg aatgttatac cccagcccaa accatttcaa
2221ctgagtttgc tgttaaattg aaaattgact tagccaaccg agagacaagc atcttcagtt
2281accgtgaaga tcccattgtc tatgaaattc atccaaccaa atcttttatt agtggtggga
2341gcacaataac aggtgttggg aaaaacctga attcagttag tgtcccgaga atggtcataa
2401atgtgcatga agcaggaagg aactttacag tggcatgtca acatcgctct aattcagaga
2461taatctgttg taccactcct tccctgcaac agctgaatct gcaactcccc ctgaaaacca
2521aagccttttt catgttagat gggatccttt ccaaatactt tgatctcatt tatgtacata
2581atcctgtgtt taagcctttt gaaaagccag tgatgatctc aatgggcaat gaaaatgtac
2641tggaaattaa gggaaatgat attgaccctg aagcagttaa aggtgaagtg ttaaaagttg
2701gaaataagag ctgtgagaat atacacttac attctgaagc cgttttatgc acggtcccca
2761atgacctgct gaaattgaac agcgagctaa atatagagtg gaagcaagca atttcttcaa
2821ccgtccttgg aaaagtaata gttcaaccag atcagaattt cacaggattg attgctggtg
2881ttgtctcaat atcaacagca ctgttattac tacttgggtt tttcctgtgg ctgaaaaaga
2941gaaagcaaat taaagatctg ggcagtgaat tagttcgcta cgatgcaaga gtacacactc
3001ctcatttgga taggcttgta agtgcccgaa gtgtaagccc aactacagaa atggtttcaa
3061atgaatctgt agactaccga gctacttttc cagaagatca gtttcctaat tcatctcaga
3121acggttcatg ccgacaagtg cagtatcctc tgacagacat gtcccccatc ctaactagtg
3181gggactctga tatatccagt ccattactgc aaaatactgt ccacattgac ctcagtgctc
3241taaatccaga gctggtccag gcagtgcagc atgtagtgat tgggcccagt agcctgattg
3301tgcatttcaa tgaagtcata ggaagagggc attttggttg tgtatatcat gggactttgt
3361tggacaatga tggcaagaaa attcactgtg ctgtgaaatc cttgaacaga atcactgaca
3421taggagaagt ttcccaattt ctgaccgagg gaatcatcat gaaagatttt agtcatccca
3481atgtcctctc gctcctggga atctgcctgc gaagtgaagg gtctccgctg gtggtcctac
3541catacatgaa acatggagat cttcgaaatt tcattcgaaa tgagactcat aatccaactg
3601taaaagatct TATTGGCTTT GGTCTTCAAG TAGCCAAAGG CATGAAATAT CTTGCAAGCA
3661AAAAGTTTGT CCACAGAGAC TTGGCTGCAA GAAACTGTAT GCTGGATGAA AAATTCACAG
3721TCAAGGTTGC TGATTTTGGT CTTGCCAGAG ACATGTATGA TAAAGAATAC TATAGTGTAC
3781ACAACAAAAC AGGTGCAAAG CTGCCAGTGA AGTGGATGGC TTTGGAAAGT CTGCAAACTC
3841AAAAGTTTAC CACCAAGTCA GATGTGTGGT CCTTTGGCGT GCTCCTCTGG GAGCTGATGA
3901CAAGAGGAGC CCCACCTTAT CCTGACGTAA ACACCTTTGA TATAACTGTT TACTTGTTGC
3961AAGGGAGAAG ACTCCTACAA CCCGAATACT GCCCAGACCC CTTATATGAA GTAATGCTAA
4021AATGCTGGCA CCCTAAAGCC GAAATGCGCC CATCCTTTTC TGAACTGGTG TCCCGGATAT
4081CAGCGATCTT CTCTACTTTC ATTGGGGAGC ACTATGTCCA TGTGAACGCT ACTTATGTGA
4141ACGTAAAATG TGTCGCTCCG TATCCTTCTC TGTTGTCATC AGAAGATAAC GCTGATGATG
4201AGGTGGACAC ACGACCAGCC TCCTTCTGGG AGACATCACC AACTTTCTTG TACaaagtgg
4261ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
4321ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)