Construct: ORF ccsbBroad304_14696
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019003.1_s304c1, BRDN0000459804
- Derived from:
- ccsbBroadEn_14696
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MET (4233)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14696
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_000245.4 | 100% | 100% | |
| 2 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_001127500.3 | 98.7% | 98.7% | 2263_2316del |
| 3 | human | 4233 | MET | MET proto-oncogene, recepto... | XM_011516223.1 | 98.6% | 98.6% | 1_57del |
| 4 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_001324402.2 | 69% | 69% | 0_1ins1290 |
| 5 | human | 4233 | MET | MET proto-oncogene, recepto... | XM_006715990.2 | 69% | 69% | 0_1ins1290 |
| 6 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_001324401.2 | 66.6% | 65.9% | (many diffs) |
| 7 | human | 4233 | MET | MET proto-oncogene, recepto... | XR_001744772.1 | 61% | 1_231del;2494_2495ins100;4302_6566del | |
| 8 | mouse | 17295 | Met | met proto-oncogene | NM_008591.2 | 85.7% | 88.9% | (many diffs) |
| 9 | mouse | 17295 | Met | met proto-oncogene | XM_011241042.2 | 85.7% | 88.9% | (many diffs) |
| 10 | mouse | 17295 | Met | met proto-oncogene | XM_011241043.2 | 85.7% | 88.9% | (many diffs) |
| 11 | mouse | 17295 | Met | met proto-oncogene | XM_006505002.3 | 82.5% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4239
- ORF length:
- 4170
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaggccccc gctgtgcttg cacctggcat cctcgtgctc ctgtttacct 121 tggtgcagag gagcaatggg gagtgtaaag aggcactagc aaagtccgag atgaatgtga 181 atatgaagta tcagcttccc aacttcaccg cggaaacacc catccagaat gtcattctac 241 atgagcatca cattttcctt ggtgccacta actacattta tgttttaaat gaggaagacc 301 ttcagaaggt tgctgagtac aagactgggc ctgtgctgga acacccagat tgtttcccat 361 gtcaggactg cagcagcaaa gccaatttat caggaggtgt ttggaaagat aacatcaaca 421 tggctctagt tgtcgacacc tactatgatg atcaactcat tagctgtggc agcgtcaaca 481 gagggacctg ccagcgacat gtctttcccc acaatcatac tgctgacata cagtcggagg 541 ttcactgcat attctcccca cagatagaag agcccagcca gtgtcctgac tgtgtggtga 601 gcgccctggg agccaaagtc ctttcatctg taaaggaccg gttcatcaac ttctttgtag 661 gcaataccat aaattcttct tatttcccag atcatccatt gcattcgata tcagtgagaa 721 ggctaaagga aacgaaagat ggttttatgt ttttgacgga ccagtcctac attgatgttt 781 tacctgagtt cagagattct taccccatta agtatgtcca tgcctttgaa agcaacaatt 841 ttatttactt cttgacggtc caaagggaaa ctctagatgc tcagactttt cacacaagaa 901 taatcaggtt ctgttccata aactctggat tgcattccta catggaaatg cctctggagt 961 gtattctcac agaaaagaga aaaaagagat ccacaaagaa ggaagtgttt aatatacttc 1021 aggctgcgta tgtcagcaag cctggggccc agcttgctag acaaatagga gccagcctga 1081 atgatgacat tcttttcggg gtgttcgcac aaagcaagcc agattctgcc gaaccaatgg 1141 atcgatctgc catgtgtgca ttccctatca aatatgtcaa cgacttcttc aacaagatcg 1201 tcaacaaaaa caatgtgaga tgtctccagc atttttacgg acccaatcat gagcactgct 1261 ttaataggac acttctgaga aattcatcag gctgtgaagc gcgccgtgat gaatatcgaa 1321 cagagtttac cacagctttg cagcgcgttg acttattcat gggtcaattc agcgaagtcc 1381 tcttaacatc tatatccacc ttcattaaag gagacctcac catagctaat cttgggacat 1441 cagagggtcg cttcatgcag gttgtggttt ctcgatcagg accatcaacc cctcatgtga 1501 attttctcct ggactcccat ccagtgtctc cagaagtgat tgtggagcat acattaaacc 1561 aaaatggcta cacactggtt atcactggga agaagatcac gaagatccca ttgaatggct 1621 tgggctgcag acatttccag tcctgcagtc aatgcctctc tgccccaccc tttgttcagt 1681 gtggctggtg ccacgacaaa tgtgtgcgat cggaggaatg cctgagcggg acatggactc 1741 aacagatctg tctgcctgca atctacaagg ttttcccaaa tagtgcaccc cttgaaggag 1801 ggacaaggct gaccatatgt ggctgggact ttggatttcg gaggaataat aaatttgatt 1861 taaagaaaac tagagttctc cttggaaatg agagctgcac cttgacttta agtgagagca 1921 cgatgaatac attgaaatgc acagttggtc ctgccatgaa taagcatttc aatatgtcca 1981 taattatttc aaatggccac gggacaacac aatacagtac attctcctat gtggatcctg 2041 taataacaag tatttcgccg aaatacggtc ctatggctgg tggcacttta cttactttaa 2101 ctggaaatta cctaaacagt gggaattcta gacacatttc aattggtgga aaaacatgta 2161 ctttaaaaag tgtgtcaaac agtattcttg aatgttatac cccagcccaa accatttcaa 2221 ctgagtttgc tgttaaattg aaaattgact tagccaaccg agagacaagc atcttcagtt 2281 accgtgaaga tcccattgtc tatgaaattc atccaaccaa atcttttatt agtggtggga 2341 gcacaataac aggtgttggg aaaaacctga attcagttag tgtcccgaga atggtcataa 2401 atgtgcatga agcaggaagg aactttacag tggcatgtca acatcgctct aattcagaga 2461 taatctgttg taccactcct tccctgcaac agctgaatct gcaactcccc ctgaaaacca 2521 aagccttttt catgttagat gggatccttt ccaaatactt tgatctcatt tatgtacata 2581 atcctgtgtt taagcctttt gaaaagccag tgatgatctc aatgggcaat gaaaatgtac 2641 tggaaattaa gggaaatgat attgaccctg aagcagttaa aggtgaagtg ttaaaagttg 2701 gaaataagag ctgtgagaat atacacttac attctgaagc cgttttatgc acggtcccca 2761 atgacctgct gaaattgaac agcgagctaa atatagagtg gaagcaagca atttcttcaa 2821 ccgtccttgg aaaagtaata gttcaaccag atcagaattt cacaggattg attgctggtg 2881 ttgtctcaat atcaacagca ctgttattac tacttgggtt tttcctgtgg ctgaaaaaga 2941 gaaagcaaat taaagatctg ggcagtgaat tagttcgcta cgatgcaaga gtacacactc 3001 ctcatttgga taggcttgta agtgcccgaa gtgtaagccc aactacagaa atggtttcaa 3061 atgaatctgt agactaccga gctacttttc cagaagatca gtttcctaat tcatctcaga 3121 acggttcatg ccgacaagtg cagtatcctc tgacagacat gtcccccatc ctaactagtg 3181 gggactctga tatatccagt ccattactgc aaaatactgt ccacattgac ctcagtgctc 3241 taaatccaga gctggtccag gcagtgcagc atgtagtgat tgggcccagt agcctgattg 3301 tgcatttcaa tgaagtcata ggaagagggc attttggttg tgtatatcat gggactttgt 3361 tggacaatga tggcaagaaa attcactgtg ctgtgaaatc cttgaacaga atcactgaca 3421 taggagaagt ttcccaattt ctgaccgagg gaatcatcat gaaagatttt agtcatccca 3481 atgtcctctc gctcctggga atctgcctgc gaagtgaagg gtctccgctg gtggtcctac 3541 catacatgaa acatggagat cttcgaaatt tcattcgaaa tgagactcat aatccaactg 3601 taaaagatct TATTGGCTTT GGTCTTCAAG TAGCCAAAGG CATGAAATAT CTTGCAAGCA 3661 AAAAGTTTGT CCACAGAGAC TTGGCTGCAA GAAACTGTAT GCTGGATGAA AAATTCACAG 3721 TCAAGGTTGC TGATTTTGGT CTTGCCAGAG ACATGTATGA TAAAGAATAC TATAGTGTAC 3781 ACAACAAAAC AGGTGCAAAG CTGCCAGTGA AGTGGATGGC TTTGGAAAGT CTGCAAACTC 3841 AAAAGTTTAC CACCAAGTCA GATGTGTGGT CCTTTGGCGT GCTCCTCTGG GAGCTGATGA 3901 CAAGAGGAGC CCCACCTTAT CCTGACGTAA ACACCTTTGA TATAACTGTT TACTTGTTGC 3961 AAGGGAGAAG ACTCCTACAA CCCGAATACT GCCCAGACCC CTTATATGAA GTAATGCTAA 4021 AATGCTGGCA CCCTAAAGCC GAAATGCGCC CATCCTTTTC TGAACTGGTG TCCCGGATAT 4081 CAGCGATCTT CTCTACTTTC ATTGGGGAGC ACTATGTCCA TGTGAACGCT ACTTATGTGA 4141 ACGTAAAATG TGTCGCTCCG TATCCTTCTC TGTTGTCATC AGAAGATAAC GCTGATGATG 4201 AGGTGGACAC ACGACCAGCC TCCTTCTGGG AGACATCACC AACTTTCTTG TACaaagtgg 4261 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 4321 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta