Construct: ORF ccsbBroad304_14741

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005783.2_s304c1, BRDN0000459717
Derived from:
ccsbBroadEn_14741
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDPK1 (5170)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14741
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5170 PDPK1 3-phosphoinositide dependen... NM_031268.6 100% 100%
2 human 5170 PDPK1 3-phosphoinositide dependen... XM_017023276.1 92.4% 87.3% (many diffs)
3 human 5170 PDPK1 3-phosphoinositide dependen... XM_011522523.3 92.2% 90.9% (many diffs)
4 human 5170 PDPK1 3-phosphoinositide dependen... XM_017023275.2 85.6% 79.6% (many diffs)
5 human 5170 PDPK1 3-phosphoinositide dependen... NM_002613.5 77.1% 76.9% 329_709del
6 human 5170 PDPK1 3-phosphoinositide dependen... XM_011522521.3 72.4% 71.1% (many diffs)
7 human 5170 PDPK1 3-phosphoinositide dependen... XM_024450296.1 72.3% 72.1% 0_1ins81;248_628del
8 human 5170 PDPK1 3-phosphoinositide dependen... XM_017023273.2 72% 68% (many diffs)
9 human 5170 PDPK1 3-phosphoinositide dependen... XM_017023272.2 70.8% 66.7% (many diffs)
10 human 5170 PDPK1 3-phosphoinositide dependen... XM_017023277.1 70.8% 64.6% (many diffs)
11 human 5170 PDPK1 3-phosphoinositide dependen... XM_017023271.2 67.7% 62.9% (many diffs)
12 human 5170 PDPK1 3-phosphoinositide dependen... XM_017023274.1 67.4% 63.3% (many diffs)
13 human 5170 PDPK1 3-phosphoinositide dependen... XM_024450295.1 67.4% 63.3% (many diffs)
14 human 5170 PDPK1 3-phosphoinositide dependen... NM_001261816.2 58.2% 57.5% (many diffs)
15 mouse 18607 Pdpk1 3-phosphoinositide dependen... XM_017317324.1 72.1% 71.3% (many diffs)
16 mouse 18607 Pdpk1 3-phosphoinositide dependen... XM_017317325.1 72.1% 71.3% (many diffs)
17 mouse 18607 Pdpk1 3-phosphoinositide dependen... XM_017317327.1 68.2% 73.4% (many diffs)
18 mouse 18607 Pdpk1 3-phosphoinositide dependen... NM_011062.4 67% 71.7% (many diffs)
19 mouse 18607 Pdpk1 3-phosphoinositide dependen... NM_001286662.1 62.4% 66.9% (many diffs)
20 mouse 18607 Pdpk1 3-phosphoinositide dependen... XM_017317320.1 62.4% 66.9% (many diffs)
21 mouse 18607 Pdpk1 3-phosphoinositide dependen... XM_017317321.1 62.4% 66.9% (many diffs)
22 mouse 18607 Pdpk1 3-phosphoinositide dependen... XM_017317322.1 62.4% 66.9% (many diffs)
23 mouse 18607 Pdpk1 3-phosphoinositide dependen... XM_017317323.1 62.4% 66.9% (many diffs)
24 mouse 18607 Pdpk1 3-phosphoinositide dependen... XM_017317326.1 62.4% 66.9% (many diffs)
25 mouse 18607 Pdpk1 3-phosphoinositide dependen... NM_001080773.2 61.2% 65.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1353
ORF length:
1287
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc caggaccacc agccagctgt atgacgccgt gcccatccag tccagcgtgg
121tgttatgttc ctgcccatcc ccatcaatgg tgaggaccca gactgagtcc agcacgcccc
181ctggcattcc tggtggcagc aggcagggcc ccgccatgga cggcactgca gccgagcctc
241ggcccggcgc cggctccctg cagcatgccc agcctccgcc gcagcctcgg aagaagcggc
301ctgaggactt caagtttggg aaaatccttg gggaaggctc tttttccacg gttgtcctgg
361ctcgagaact ggcaacctcc agagaatatg cgaccagggc caactcattc gtgggaacag
421cgcagtacgt ttctccagag ctgctcacgg agaagtccgc ctgtaagagt tcagaccttt
481gggctcttgg atgcataata taccagcttg tggcaggact cccaccattc cgagctggaa
541acgagtatct tatatttcag aagatcatta agttggaata tgactttcca gaaaaattct
601tccctaaggc aagagacctc gtggagaaac ttttggtttt agatgccaca aagcggttag
661gctgtgagga aatggaagga tacggacctc ttaaagcaca cccgttcttc gagtccgtca
721cgtgggagaa cctgcaccag cagacgcctc cgaagctcac cgcttacctg ccggctatgt
781cggaagacga cgaggactgc tatggcaatt atgacaatct ccTGAGCCAG TTTGGCTGCA
841TGCAGGTGTC TTCGTCCTCC TCCTCACACT CCCTGTCAGC CTCCGACACG GGCCTGCCCC
901AGAGGTCAGG CAGCAACATA GAGCAGTACA TTCACGATCT GGACTCGAAC TCCTTTGAAC
961TGGACTTACA GTTTTCCGAA GATGAGAAGA GGTTGTTGTT GGAGAAGCAG GCTGGCGGAA
1021ACCCTTGGCA CCAGTTTGTA GAAAATAATT TAATACTAAA GATGGGCCCA GTGGATAAGC
1081GGAAGGGTTT ATTTGCAAGA CGACGACAGC TGTTGCTCAC AGAAGGACCA CATTTATATT
1141ATGTGGATCC TGTCAACAAA GTTCTGAAAG GTGAAATTCC TTGGTCACAA GAACTTCGAC
1201CAGAGGCCAA GAATTTTAAA ACTTTCTTTG TCCACACGCC TAACAGGACG TATTATCTGA
1261TGGACCCCAG CGGGAACGCA CACAAGTGGT GCAGGAAGAT CCAGGAGGTT TGGAGGCAGC
1321GATACCAGAG CCACCCGGAC GCCGCTGTGC AGTGCCCAAC TTTCTTGTAC aaagtggttg
1381gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
1441accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)