Construct: ORF ccsbBroad304_14741
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005783.2_s304c1, BRDN0000459717
- Derived from:
- ccsbBroadEn_14741
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDPK1 (5170)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14741
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | NM_031268.6 | 100% | 100% | |
| 2 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023276.1 | 92.4% | 87.3% | (many diffs) |
| 3 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_011522523.3 | 92.2% | 90.9% | (many diffs) |
| 4 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023275.2 | 85.6% | 79.6% | (many diffs) |
| 5 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | NM_002613.5 | 77.1% | 76.9% | 329_709del |
| 6 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_011522521.3 | 72.4% | 71.1% | (many diffs) |
| 7 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_024450296.1 | 72.3% | 72.1% | 0_1ins81;248_628del |
| 8 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023273.2 | 72% | 68% | (many diffs) |
| 9 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023272.2 | 70.8% | 66.7% | (many diffs) |
| 10 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023277.1 | 70.8% | 64.6% | (many diffs) |
| 11 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023271.2 | 67.7% | 62.9% | (many diffs) |
| 12 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023274.1 | 67.4% | 63.3% | (many diffs) |
| 13 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_024450295.1 | 67.4% | 63.3% | (many diffs) |
| 14 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | NM_001261816.2 | 58.2% | 57.5% | (many diffs) |
| 15 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317324.1 | 72.1% | 71.3% | (many diffs) |
| 16 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317325.1 | 72.1% | 71.3% | (many diffs) |
| 17 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317327.1 | 68.2% | 73.4% | (many diffs) |
| 18 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | NM_011062.4 | 67% | 71.7% | (many diffs) |
| 19 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | NM_001286662.1 | 62.4% | 66.9% | (many diffs) |
| 20 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317320.1 | 62.4% | 66.9% | (many diffs) |
| 21 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317321.1 | 62.4% | 66.9% | (many diffs) |
| 22 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317322.1 | 62.4% | 66.9% | (many diffs) |
| 23 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317323.1 | 62.4% | 66.9% | (many diffs) |
| 24 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317326.1 | 62.4% | 66.9% | (many diffs) |
| 25 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | NM_001080773.2 | 61.2% | 65.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1353
- ORF length:
- 1287
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc caggaccacc agccagctgt atgacgccgt gcccatccag tccagcgtgg 121 tgttatgttc ctgcccatcc ccatcaatgg tgaggaccca gactgagtcc agcacgcccc 181 ctggcattcc tggtggcagc aggcagggcc ccgccatgga cggcactgca gccgagcctc 241 ggcccggcgc cggctccctg cagcatgccc agcctccgcc gcagcctcgg aagaagcggc 301 ctgaggactt caagtttggg aaaatccttg gggaaggctc tttttccacg gttgtcctgg 361 ctcgagaact ggcaacctcc agagaatatg cgaccagggc caactcattc gtgggaacag 421 cgcagtacgt ttctccagag ctgctcacgg agaagtccgc ctgtaagagt tcagaccttt 481 gggctcttgg atgcataata taccagcttg tggcaggact cccaccattc cgagctggaa 541 acgagtatct tatatttcag aagatcatta agttggaata tgactttcca gaaaaattct 601 tccctaaggc aagagacctc gtggagaaac ttttggtttt agatgccaca aagcggttag 661 gctgtgagga aatggaagga tacggacctc ttaaagcaca cccgttcttc gagtccgtca 721 cgtgggagaa cctgcaccag cagacgcctc cgaagctcac cgcttacctg ccggctatgt 781 cggaagacga cgaggactgc tatggcaatt atgacaatct ccTGAGCCAG TTTGGCTGCA 841 TGCAGGTGTC TTCGTCCTCC TCCTCACACT CCCTGTCAGC CTCCGACACG GGCCTGCCCC 901 AGAGGTCAGG CAGCAACATA GAGCAGTACA TTCACGATCT GGACTCGAAC TCCTTTGAAC 961 TGGACTTACA GTTTTCCGAA GATGAGAAGA GGTTGTTGTT GGAGAAGCAG GCTGGCGGAA 1021 ACCCTTGGCA CCAGTTTGTA GAAAATAATT TAATACTAAA GATGGGCCCA GTGGATAAGC 1081 GGAAGGGTTT ATTTGCAAGA CGACGACAGC TGTTGCTCAC AGAAGGACCA CATTTATATT 1141 ATGTGGATCC TGTCAACAAA GTTCTGAAAG GTGAAATTCC TTGGTCACAA GAACTTCGAC 1201 CAGAGGCCAA GAATTTTAAA ACTTTCTTTG TCCACACGCC TAACAGGACG TATTATCTGA 1261 TGGACCCCAG CGGGAACGCA CACAAGTGGT GCAGGAAGAT CCAGGAGGTT TGGAGGCAGC 1321 GATACCAGAG CCACCCGGAC GCCGCTGTGC AGTGCCCAAC TTTCTTGTAC aaagtggttg 1381 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1441 accggtggcg cgttaagtcg acaatcaacc tctggatta