Construct: ORF ccsbBroad304_14751

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018907.1_s304c1, BRDN0000459391
Derived from:
ccsbBroadEn_14751
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PHKA1 (5255)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14751
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5255 PHKA1 phosphorylase kinase regula... NM_001122670.1 99.5% 20.9% (many diffs)
2 human 5255 PHKA1 phosphorylase kinase regula... NM_002637.4 98.5% 20.7% (many diffs)
3 human 5255 PHKA1 phosphorylase kinase regula... NM_001172436.1 94.6% 22% (many diffs)
4 human 5255 PHKA1 phosphorylase kinase regula... XM_006724661.2 93.6% 21.7% (many diffs)
5 human 5255 PHKA1 phosphorylase kinase regula... XR_001755696.1 53.2% (many diffs)
6 mouse 18679 Phka1 phosphorylase kinase alpha 1 XM_006527894.3 89.8% 20.5% (many diffs)
7 mouse 18679 Phka1 phosphorylase kinase alpha 1 NM_008832.2 88.8% 20.3% (many diffs)
8 mouse 18679 Phka1 phosphorylase kinase alpha 1 NM_173021.3 84.4% 21.3% (many diffs)
9 mouse 18679 Phka1 phosphorylase kinase alpha 1 XM_006527895.3 83.1% 21.7% (many diffs)
10 mouse 18679 Phka1 phosphorylase kinase alpha 1 XM_017318427.1 60.5% 31.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
879
ORF length:
810
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaggagccgg agtaactccg gggtccggct ggacggctac gctcgactgg
121tgcaacagac catcctgtgc catcagaatc cagtgactgg cttgcttcca gccagctatg
181atcagaaaga tgcttgggtc cgagataatg tgtacagcat cttggctgtg tggggtttgg
241gcctggccta tcggaagaat gcagaccggg atgaggataa ggcaaaggcc tatgaattgg
301agcagagtgt agtgaagctg atgagaggac tactgcactg catgatcaga caggtggata
361aagtagaatc cttcaaatat agtcagagta ctaaggatag cctccatgca aagtacaaca
421ccaaaacctg tgccactgta gtgggtgatg atcaatgggg acacctgcag ttggatgcta
481cctctgtgta cctgctcttc ttagcccaaa tgactgcctc aggactccat atcatccaca
541gcctagatga agtcaatttc atacagaacc ttgtgtttta cattgaagct gcatataaaa
601ctgctgactt cgggatatgg gaacgtggag acaagaccaa ccaagggatc tcagagttga
661atgccagttc agttggaatg gcaaaggcag ccctggaagc attagatgaa ctggatctgt
721ttggtgtgaa aggtgggcct caatcagtta tccatgtcct ggctgatgaa gtacagcact
781gccagtctat cctaaattca ctactgcccc gtgcttcaca tcaaagagtg atgctagtct
841actctcagtg gtttccttcc ctgccttgca gtagagggta gccagttggt ggagctcaca
901aaacaggaaa tcatcaccaa gcttcagggt cgttatggtt gctgtcgctt tctacgagat
961ggatataaaa ctcctaaaga ggatcccaat cgtctgtact atgaaccagc tgagctgaag
1021ctatttgaaa acattgagtg tgaatggcca ttgttctgga catactttat tcttgatggg
1081gtcttcagtg gcaatgcaga acaggttcaa gaatataaag aggctcttga agcagtcctc
1141atcaagggca aaaatggagt cccacttctg ccagagctgt acagtgttcc tcctgacagg
1201gtcgatgaag aatatcagaa tcctcacact gtggaccgag tccccatggg gaaattgcct
1261cacatgtggg gtcagtctct atacatttta ggaagcttga tggcagaggg atttttagcc
1321cctggagaaa ttgatcccct gaatcgcagg ttttctactg taccgaagcc cgatgttgtg
1381gttcaagtct ccattctagc tgaaacagaa gaaatcaaga ccattttgaa ggacaaggga
1441atttacgtgg agaccattgc tgaggtatac cccatcagag tacaaccagc tcgtattctc
1501agccacattt attccagcct aggatgcaac aatagaatga aactcagtgg acgaccctac
1561agacacatgg gagtgcttgg aacttcaaaa ctctatgaca ttcggaaaac tatctttact
1621ttcactccac agtttataga ccagcaacag ttctacctgg ctctggacaa caagatgata
1681gtggaaatgc ttagaacaga cctctcctac ctctgtagcc gctggcggat gacaggccag
1741cccaccatca ccttccccat ctcatacagc atgcttgatg aagatggaac aagcttgaat
1801tcaagtatcc tggcagcact ccgaaaaatg caagatgggt attttggtgg ggcaagggtt
1861caaacaggta aattgtcaga gtttttgaca acatcttgtt gcacacactt gagcttcatg
1921gaccctggac ctgagggtaa gctgtacagt gaagattatg atgacaacta tgattacctg
1981gaatctggca actggatgaa tgattatgat tcaaccagtc atgctcgctg tggtgatgaa
2041gttgctcgtt atttagatca ccttttggcg cacactgctc cccatcctaa actagcccct
2101acctcacaga agggagggct agatcggttc caagctgctg tgcaaacaac ctgcgactta
2161atgtccttgg tgaccaaggc caaggaactg catgtacaga atgttcacat gtatcttcct
2221acgaagttat ttcaggcttc ccggccttca ttcaacttac ttgattcacc tcatccccga
2281caggagaacc aggttccctc tgttcgtgta gaaatacatc ttcctagaga ccagtctggg
2341gaggtggact ttaaagcact ggttttacag ttgaaggaga cctcaagctt acaggaacaa
2401gctgatatcc tctatatgct gtatactatg aaaggacctg actggaacac tgaattgtat
2461aatgaacgga gtgctacagt gagagagctt cttaccgagc tgtatggcaa agtgggagaa
2521attcgtcact ggggcctgat ccgatacatt tctgggatct taaggaagaa agtggaagca
2581cttgatgagg cctgcacaga ccttctctcc caccagaaac atttgacagt aggacttcct
2641ccagaacctc gagaaaagac tatctctgca cctctgccct atgaggcgct cactcagctg
2701atagatgaag ccagtgaagg ggatatgagc atttcaatcc ttacacagga aataatggta
2761tatctagcca tgtatatgcg aacccagcct ggcctctttg ctgaaatgtt tcgacttcga
2821attggtctga tcatacaagt tatggcaaca gaactggccc actcccttcg atgctcagct
2881gaggaagcca cagagggcct gatgaatctc agtccttcgc ccatgaagga tctcctgcat
2941cacattctca gccgcaagga gttggagtgg gaacgaagcg ttcgtcccac tgatcaaaat
3001gtcagtcctg ctatttctat ccacgagatt ggtgctgtgg agcaaccaaa acagaacgaa
3061ctgggatcat gcagttaaaa agtgagataa agcagtcacc tggaacctct atgactccaa
3121gtagtgggtc ctttcctagt gcatatgatc agcagtcatc taaagatagt cgtcaaggtc
3181aatggcaacg ccgaagaagg ctggatgggg cactgaatag agttccagtt ggattttatc
3241agaaagtatg gaaagttttg cagaagtgtc acggactttc tgttgaaggg tttgtccttc
3301cttcctctac cactagagag atgactccag gtgagattaa attctctgtt catgtggagt
3361ctgtcctgaa tcgtgtacct cagccagagt accgtcagct gctggttgaa gccatccttg
3421tcctcaccat gctggcagat attgaaattc atagcatcgg aagcatcatt gctgtggaaa
3481aaatagtgca tattgccaat gacttgttcc ttcaagaaca gaaaaccctt ggcgcagatg
3541ataccatgtt ggcaaaggat cccgcatctg gcatctgtac tcttctgtat gacagtgcac
3601ccagtggcag gtttggcacc atgacctacc tctccaaggc agccgccacc tacgtgcagg
3661agttcctgcc tcacagcatc tgtgccatgc aattgccaac tttcttgtac aaagtggttg
3721gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
3781accggtggcg cgttaagtcg acaatcaacc tctggatta

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