Construct: ORF ccsbBroad304_14801
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018876.1_s304c1, BRDN0000459360
- Derived from:
- ccsbBroadEn_14801
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAPK4 (5596)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14801
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5596 | MAPK4 | mitogen-activated protein k... | NM_001292040.2 | 80.3% | 4.6% | (many diffs) |
| 2 | human | 5596 | MAPK4 | mitogen-activated protein k... | NM_002747.4 | 43.3% | 1.8% | (many diffs) |
| 3 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_005258299.3 | 43.3% | 1.8% | (many diffs) |
| 4 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526074.2 | 43.3% | 1.8% | (many diffs) |
| 5 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526075.3 | 43.3% | 1.8% | (many diffs) |
| 6 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526076.2 | 43.3% | 1.8% | (many diffs) |
| 7 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_017025839.2 | 43.3% | 1.8% | (many diffs) |
| 8 | mouse | 225724 | Mapk4 | mitogen-activated protein k... | NM_172632.2 | 39.4% | 1.6% | (many diffs) |
| 9 | mouse | 225724 | Mapk4 | mitogen-activated protein k... | XM_006525900.3 | 39.4% | 1.6% | (many diffs) |
| 10 | mouse | 225724 | Mapk4 | mitogen-activated protein k... | XM_006525901.3 | 39.4% | 1.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 107
- ORF end:
- 215
- ORF length:
- 108
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ntngccancc anggntgaga anggtggact gcatcgccnn ngtcttatgg gtatgacnnn 121 gntgggcgct ttgttnactt cnaacccctg ggnntcggtg tcaatggttt ggtgctgtcg 181 gccgtggaca gccgggcctg ccggaaggtc gctgtgaaga agattgccct gagcgatgcc 241 cgcagcatga agcacgcgct ccgagagatc aagatcattc ggcgcctgga ccacgacaac 301 atcgtcaaag tgtacgaggt gctcggtccc aagggcactg acctgcaggg tgagctgttc 361 aagttcagcg tggcgtacat cgtccaggag tacatggaga ccgacctggc acgcctgctg 421 gagcagggca cgctggcaga agagcatgcc aagctgttca tgtaccagct gctccgcggg 481 ctcaagtaca tccactccgc caacgtgctg cacagggacc tgaagcccgc caacatcttc 541 atcagcacag aggacctcgt gctcaagatt ggggatttcg ggttggcaag gatcgttgat 601 cagcattact cccacaaggg ttatctgtca gaagggttgg taacaaagtg gtaccgttcc 661 ccggccgccc ggccccggtg gacggcggcg ccagccccca gttcgacctg gacgtgttca 721 tctcccgcgc cctgaagctc tgcaccaagc ccgaggacct gccggacaat naactgggcg 781 acctcaatgg tgcgtgcatc cccgagcacc ctggcgacct cgtgcagacc gaggccttct 841 cnnaagaaag gtggttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 901 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 961 cgacaatcaa cctctggatt a