Construct: ORF ccsbBroad304_14831
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018965.1_s304c1, BRDN0000459449
- Derived from:
- ccsbBroadEn_14831
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RPS6KA2 (6196)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14831
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_021135.6 | 99.3% | 34.6% | (many diffs) |
| 2 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | XM_006715549.3 | 95.6% | 33.4% | (many diffs) |
| 3 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001006932.3 | 95.2% | 29.8% | (many diffs) |
| 4 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001318936.2 | 94.2% | 29.1% | (many diffs) |
| 5 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001318938.1 | 87.2% | 23% | (many diffs) |
| 6 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001318937.2 | 85.4% | 20.7% | (many diffs) |
| 7 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | NM_011299.4 | 87.2% | 33.5% | (many diffs) |
| 8 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523199.3 | 84.1% | 30% | (many diffs) |
| 9 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523198.3 | 84.1% | 30.3% | (many diffs) |
| 10 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523200.1 | 74.4% | 20.4% | (many diffs) |
| 11 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523201.1 | 74.4% | 20.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1023
- ORF length:
- 957
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cctgagcatg aagaagttcg ccgtgcgcag gttcttctct gtgtacctgc 121 gcaggaagtc gcgctccaag agctccagcc tgagccggct cgaggaagaa ggtgtcgtga 181 aggagataga catcagccat catgtgaagg agggctttga gaaggcagat ccttcccagt 241 ttgagctgct gaaggtttta ggacaaggat cctatggaaa ggtgttcctg gtgaggaagg 301 tgaaggggtc cgacgctggg cagctctacg ccatgaaggt ccttaagaaa gccaccctaa 361 aagttcggga ccgagtgaga tcgaagatgg agagagacat cttggcagaa gtgaatcacc 421 ccttcattgt gaagcttcat tatgcctttc agacggaagg aaagctctac ctgatcctgg 481 acttcctgcg gggaggggac ctcttcaccc ggctctccaa agaggtcatg ttcacggagg 541 aggatgtcaa gttctacctg gctgagctgg ccttggcttt agaccatctc cacagcctgg 601 ggatcatcta cagagatctg aagcctgaga acatcctcct ggatgaagag gggcacatta 661 agatcacaga tttcggcctg agtagggagg ccattgacca cgacaagaga gcgtactcct 721 tctgcgggac gatcgagtac atggcgcccg aggtggtgaa ccggcgagga cacacgcaga 781 gtgccgactg gtggtccttc ggcgtgctca tgtttgagat gctcacgggg tccctgccgt 841 tccagggaaa ggaccaggaa aggaggccat ggctctcatc ctcaaagcca agctggggat 901 gccgcagttc ctcagtgggg aggcacagag tttgctgcga gctctcttca aacggaaccc 961 ctgcaaccgg ctgggtgctg gcattgacgg agtggaggaa attaagcgcc atcccttctt 1021 tgtgaccata gactggaaca cgctgtaccg gaaggagatc aagccaccgt tcaaaccagc 1081 agtgggcagg cctgaggaca ccttccactt tgaccccgag ttcacagcgc ggacgcccac 1141 agactctcct ggcgtccccc cgagtgcaaa cgctcatcac ctgtttagag gattcagctt 1201 tgtggcctca agcctgatcc aggagccctc acagcaagat ctgcacaaag tcccagttca 1261 cccaatcgtg cagcagttac acgggaacaa catccacttc accgatggct acgagatcaa 1321 ggaggacatc ggggtgggct cctactcagt gtgcaagcga tgtgtgcata aagccacaga 1381 caccgagtat gccgtgaaga tcattgataa gagcaagaga gacccctcgg aagagattga 1441 gatcctcctg cggtacggcc agcacccgaa catcatcacc ctcaaaggat gttatgatga 1501 tgccaagttt gtgtacctgg taatggagct gatgcgtggt ggggagctcc tggaccgcat 1561 cttccgggca gagatacttc tcggagcgcg aagccagtga cgtcctgtgc accatcacca 1621 agaccatgga ctacctccat tcccaggggg tgtccatcga gacctgaagc cgagtaacat 1681 cctgtacagg gatgagtcgg ggagcccaga atccatccga gtctgcgact tcggctttgc 1741 caagcagctg cgcgcgggga acgggctgct catgacaccc tgctacacgg ccaatttcgt 1801 ggccccggag gtcctgaagc gtcaaggcta tgatgcggcg tgtgacatct ggagtttggg 1861 gatcctgttg tacaccatgc tggcaggatt tacccctttt gcaaatgggc cagacgatac 1921 ccctgaggag attctggcgc ggatcggcag tgggaagtat gccctttctg ggggaaactg 1981 ggactcgata tctgacgcag ctaaagacgt cgtgtccaag atgctccacg tggaccctca 2041 tcagcgcctg acggcgatgc aagtgctcaa acacccgtgg gtggtcaaca gagagtacct 2101 gtccccaaac cagctcagcc gacaggacgt gcacctggtg aagggcgcga tggccgccac 2161 ctactttgct ctaaacagaa cacctcaggc cccgcggctg gagcccgtgc tgtcgtccaa 2221 cctggctcag cgcagaggca tgaagagact cacgtccacg cggttgtacc caactttctt 2281 gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta 2341 atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga tta