Construct: ORF ccsbBroad304_14892
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013050.2_s304c1, BRDN0000459764
- Derived from:
- ccsbBroadEn_14892
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIP5K1B (8395)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14892
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | NM_001278253.1 | 91.3% | 90.8% | (many diffs) |
| 2 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_006717301.1 | 91.3% | 90.8% | (many diffs) |
| 3 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_017015191.1 | 91.3% | 90.8% | (many diffs) |
| 4 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | NM_003558.4 | 87.2% | 86.2% | (many diffs) |
| 5 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_005252261.3 | 87.2% | 86.2% | (many diffs) |
| 6 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_005252262.5 | 87.2% | 86.2% | (many diffs) |
| 7 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_006717300.2 | 87.2% | 86.2% | (many diffs) |
| 8 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_011519082.2 | 87.2% | 86.2% | (many diffs) |
| 9 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_011519083.2 | 87.2% | 86.2% | (many diffs) |
| 10 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_011519084.3 | 87.2% | 86.2% | (many diffs) |
| 11 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_017015188.1 | 87.2% | 86.2% | (many diffs) |
| 12 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_017015189.1 | 87.2% | 86.2% | (many diffs) |
| 13 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_017015190.1 | 87.2% | 86.2% | (many diffs) |
| 14 | mouse | 18719 | Pip5k1b | phosphatidylinositol-4-phos... | NM_008846.3 | 76.9% | 83.4% | (many diffs) |
| 15 | mouse | 18719 | Pip5k1b | phosphatidylinositol-4-phos... | XM_006526763.3 | 76.9% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1713
- ORF length:
- 1647
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttctgctgct gaaaatggag aggcagcacc tggaaaacaa aatgaagaaa 121 aaacctataa aaagacacaa gtgaaaaacc aaatcctgtc tcgtttaccc aagattccgt 181 gtacattcat tcaagcatgg accaacacag agatgattac gttgctagcc tgttattact 241 tcagatcagt ttcaactgca tcatctgcta ttaaaggtgc tattcagctg ggaataggat 301 acacagtggg taatctcact tccaagccag aacgagatgt tcttatgcaa gacttttatg 361 tggtggaaag tgtgttccta cccagcgaag ggagcaatct gaccccagca catcactacc 421 cagactttag atttaagaca tacgctccat tagcattccg atatttcaga gaactttttg 481 gtatcaagcc tgatgattac ttgtattcca tctgcagtga acctctaata gaactgtcta 541 accctggagc cagtggatcc ttgttttttg tgaccagtga tgatgaattt atcatcaaaa 601 cagttcagca caaagaagct gagtttcttc agaagctact gccaggctat tacatgaatt 661 taaaccagaa tccaaggact cttttgccaa aattttacgg actgtattgt atgcaatcag 721 gaggcattaa tatcaggatt gtggtgatga acaacgtttt gccacgctcc atgagaatgc 781 actttacata tgacttgaaa ggctcaacgt ataagcgaag agcatcccgt aaagagagag 841 agaaatccaa ccccacattt aaggacttag atttcctgca agacatgcac gaagggttgt 901 attttgatac ggaaacatac aacgcgctta tgaaaacact tcagagagac tgccgggtgc 961 tagaaagctt caagatcatg gattatagcc ttctgttggg aattcatttc ctggaccatt 1021 ccctcaaaga gaaagaggag gagaccccac aaaatgtgcc tgatgctaag cggactggga 1081 tgcagaaggt tctctactca acagccatgg aatctatcca gggtccaggg aaatctggag 1141 atgggataat cacagagaac ccagacacaa tgggaggcat tccagctaaa agccataggg 1201 gagaaaaact acttttattt acgggcatta ttgacattct gcaatcatat aggttaatga 1261 agaagttaga acattcctgg aaagctcttg tttatgatgg ggacactgtt tctgttcata 1321 gaccaagctt ttatgcagac agatttctta agttcatgaa ttccagagtt ttcaagaaaa 1381 ttcaagcttt gaaggcttca ccgtctaaga aacggtgcaa ttcaatcgcc gccctaaagg 1441 ccacttcaca ggagattgtg tcctcaatta gccaggaatg gaaggatgag aagcgggatt 1501 tgctgactga aggacaaagt tttagcagcc ttgatgaaga agccctggga tcccgacaca 1561 ggccagacct ggtccctagc actccatcac tgtttgaagc tgcttccttg gcaaccacaa 1621 tttcatcttc ttccttatac gtcaatgagc actatccaca cgacaggcct acactctatt 1681 caaacaggtt caagatggca acatcagagc attacccaac tttcttgtac aaagtggttg 1741 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1801 accggtggcg cgttaagtcg acaatcaacc tctggatta