Construct: ORF ccsbBroad304_14900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018900.1_s304c1, BRDN0000459384
- Derived from:
- ccsbBroadEn_14900
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DGKZ (8525)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14900
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_001199267.2 | 97.9% | 28.6% | (many diffs) |
| 2 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_003646.4 | 97.8% | 28.6% | (many diffs) |
| 3 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_001199266.2 | 97.2% | 28.4% | (many diffs) |
| 4 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_001199268.2 | 95.3% | 26.1% | (many diffs) |
| 5 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448738.1 | 94.5% | 27.6% | (many diffs) |
| 6 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448737.1 | 94.4% | 27.6% | (many diffs) |
| 7 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_005253182.3 | 94.1% | 23.5% | (many diffs) |
| 8 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_201533.3 | 94% | 23.4% | (many diffs) |
| 9 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_201532.3 | 93.2% | 23.4% | (many diffs) |
| 10 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448736.1 | 91% | 22.7% | (many diffs) |
| 11 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448735.1 | 90.9% | 22.7% | (many diffs) |
| 12 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448734.1 | 90.1% | 22.6% | (many diffs) |
| 13 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448733.1 | 90% | 22.6% | (many diffs) |
| 14 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448739.1 | 88.6% | 20.4% | (many diffs) |
| 15 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448741.1 | 82% | 14.9% | (many diffs) |
| 16 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448740.1 | 81.7% | 22.6% | (many diffs) |
| 17 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_001105540.2 | 80.2% | 19.9% | (many diffs) |
| 18 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448732.1 | 77.9% | 19.3% | (many diffs) |
| 19 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_017018456.1 | 77.4% | 22.5% | (many diffs) |
| 20 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_017018455.1 | 77.3% | 22.5% | (many diffs) |
| 21 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448730.1 | 75.3% | 21.9% | (many diffs) |
| 22 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448729.1 | 75.2% | 21.9% | (many diffs) |
| 23 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448731.1 | 73.3% | 20% | (many diffs) |
| 24 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XR_002957206.1 | 61.4% | (many diffs) | |
| 25 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | NM_138306.2 | 86.8% | 27% | (many diffs) |
| 26 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498542.3 | 85.7% | 26.6% | (many diffs) |
| 27 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498543.2 | 83.9% | 22.1% | (many diffs) |
| 28 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498541.2 | 82.9% | 22.7% | (many diffs) |
| 29 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498540.2 | 82.8% | 21.8% | (many diffs) |
| 30 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498539.1 | 81.9% | 22.3% | (many diffs) |
| 31 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_017314875.1 | 74.3% | 14.8% | (many diffs) |
| 32 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_017314878.1 | 74.3% | 14.8% | (many diffs) |
| 33 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | NM_001166597.1 | 70.7% | 18.6% | (many diffs) |
| 34 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_017314867.1 | 70.6% | 18.6% | (many diffs) |
| 35 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498538.2 | 70% | 18.4% | (many diffs) |
| 36 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498537.1 | 69.9% | 18.4% | (many diffs) |
| 37 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_011239240.1 | 60.2% | 21.1% | (many diffs) |
| 38 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_011239241.2 | 41.3% | 28.4% | (many diffs) |
| 39 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_011239244.1 | 37.8% | 30.1% | (many diffs) |
| 40 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XR_374381.2 | 28.5% | (many diffs) | |
| 41 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XR_866203.1 | 23.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 894
- ORF length:
- 825
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagccgcgg gacggtagcc ccgaggcccg gagcagcgac tccgagtcgg 121 cttccgcctc gtccagcggc tccgagcgcg acgccggtcc cgagccggac aaggcgccgc 181 ggcgactcaa caagcggcgc ttcccggggc tgcggctctt cgggcacagg aaagccatca 241 ccaagtcggg cctccagcac ctggcccccc ctccgcccac ccctggggcc ccgtgcagcg 301 agtcagagcg gcagatccgg agtacagtgg actggagcga gtcagcgaca tatggggagc 361 acatctggtt cgagaccaac gtgtccgggg acttctgcta cgttggggag cagtactgtg 421 tagccaggat gctgaagtca gtgtctcgaa gaaagtgcgc agcctgcaag attgtggtgc 481 acacgccctg catcgagcag ctggagaaga taaatttccg ctgtaagccg tccttccgtg 541 aatcaggctc caggaatgtc cgcgagccaa cctttgtacg gcaccactgg gtacacagac 601 gacgccagga cggcaagtgt cggcactgtg ggaagggatt ccagcagaag ttcaccttcc 661 acagcaagga gattgtggcc atcagctgct cgtggtgcaa gcaggcatac cacagcaagg 721 tgtcctgctt catgctgcag cagatcgagg agccgtgctc gctgggggtc cacgcagccg 781 tggtcatccc gcccacctgg atcctccgcg cccggaggcc ccagaatact ctgaaagcaa 841 gcaagaagaa gaagagggca tcctttcaag aggaagtcca gcaagaaagg gcctgaggag 901 ggccgctgga gacccttcat catcaggccc accccctccc cgctcatgaa gcccctgctg 961 gtgtttgtga accccaagag tgggggcaac cagggtgcaa agatcatcca gtctttcctc 1021 tggtatctca atccccgaca agtcttcgac ctgagccagg gagggcccaa ggaggcgctg 1081 gagatgtacc gcaaagtgca caacctgcgg atcctggcgt gcgggggcga cggcacggtg 1141 ggctggatcc tctccaccct ggaccagcta cgcctgaagc cgccaccccc tgttgccatc 1201 ctgcccctgg gtactggcaa cgacttggcc cgaaccctca actggggtgg gggctacaca 1261 gatgagcctg tgtccaagat cctctcccac gtggaggagg ggaacgtggt acagctggac 1321 cgctgggacc tccacgctga gcccaacccc gaggcagggc ctgaggaccg agatgaaggc 1381 gccaccgacc ggttgcccct ggatgtcttc aacaactact tcagcctggg ctttgacgcc 1441 cacgtcaccc tggagttcca cgagtctcga gaggccaacc cagagaaatt caacagccgc 1501 tttcggaata agatgttcta cgccgggaca gctttctctg acttcctgat gggcagctcc 1561 aaggacctgg ccaagcacat ccgagtggtg tgtgatggaa tggacttgac tcccaagatc 1621 caggacctga aacccaagtg tgttgttttc ctgaacatcc ccaggtactg tgcgggcacc 1681 atgccctggg gccaccctgg ggagcaccac gactttgagc cccagcggca tgacgacggc 1741 tacctcgagg tcattggctt caccatgacg tcgttggccg cgctgcaggt gggcggacac 1801 ggcgagcggc tgacgcagtg tcgcgaggtg gtgctcacca catccaaggc catcccggtg 1861 caggtggatg gcgagccctg caagcttgca gcctcacgca tccgcatcgc cctgcgcaac 1921 caggccacca tggtgcagaa ggccaagcgg cggagcgccg cccccctgca cagcgaccag 1981 cagccggtgc cagagcagtt gcgcatccag gtgagtcgcg tcagcatgca cgactatgag 2041 gccctgcctt acgacaaggg agcaattaaa gggaggcctt tttcccgctg ggcaatgttg 2101 tggttccagg gagacatgac cttagaactt tgcccgggcc cacatgaaga gattccaaca 2161 ggagccccga tgtgcgggag gccaagtccc gaacatgcca gaaatgtccc ccaagtggtg 2221 tttcctggac gccaccactg ccagccgttt ctacaggatc gaccgagccc aggagcacct 2281 caactatgtg actgagatcg caccggcctg aggttgnttt ccnggccccc gngcngcngg 2341 gggcttcggc cccncccnna cctcccaccc ccccaccttc ccctctcccc acctcaccct 2401 gctcacccac gccccggtca ctgcaagggg atgctgcacc ccctcaaggt gaagagctga 2461 ttgaggctgc caagaggaac gacttctgta agctccagga gctgcaccga gctgggggcg 2521 acctcatgca ccgagacgag cagagtcgca cgctcctgca ccacgcagtc agcactggca 2581 gcaaggatgt ggtccgctac ctgctggacc acgccccccc agagatcctt gatgcggtgg 2641 aggaaaacgg ggagacctgt ttgcaccaag cagcggccct gggccagcgc accatctgcc 2701 actacatcgt ggaggccggg gcctcgctca tgaagacaga ccagcagggc gacactcccc 2761 ggcagcgggc tgagaaggct caggacaccg agctggccgc ctacctggag aaccggcagc 2821 actaccagat gatccagcgg gaggaccagg agacggctgt gttgccaact ttcttgtaca 2881 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2941 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta