Construct: ORF ccsbBroad304_14923

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018868.1_s304c1, BRDN0000459352
Derived from:
ccsbBroadEn_14923
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
BRSK2 (9024)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14923
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9024 BRSK2 BR serine/threonine kinase 2 NM_003957.4 96.5% 37.1% (many diffs)
2 human 9024 BRSK2 BR serine/threonine kinase 2 NM_001256629.2 93.4% 36.7% (many diffs)
3 human 9024 BRSK2 BR serine/threonine kinase 2 XM_005253218.3 88.7% 34.4% (many diffs)
4 human 9024 BRSK2 BR serine/threonine kinase 2 NM_001256627.2 87.5% 33.6% (many diffs)
5 human 9024 BRSK2 BR serine/threonine kinase 2 XM_006718374.2 85.5% 32.7% (many diffs)
6 human 9024 BRSK2 BR serine/threonine kinase 2 NM_001282218.2 84.7% 27.8% (many diffs)
7 human 9024 BRSK2 BR serine/threonine kinase 2 NM_001256630.1 81.9% 29.8% (many diffs)
8 human 9024 BRSK2 BR serine/threonine kinase 2 XM_011520462.2 80.5% 26.1% (many diffs)
9 human 9024 BRSK2 BR serine/threonine kinase 2 XM_011520461.2 79.4% 25.5% (many diffs)
10 human 9024 BRSK2 BR serine/threonine kinase 2 XM_006718376.2 77.7% 24.8% (many diffs)
11 human 9024 BRSK2 BR serine/threonine kinase 2 XM_017018536.1 76.1% 28.9% (many diffs)
12 human 9024 BRSK2 BR serine/threonine kinase 2 XM_017018533.1 74.6% 28.1% (many diffs)
13 human 9024 BRSK2 BR serine/threonine kinase 2 XM_017018537.1 73.7% 23.1% (many diffs)
14 human 9024 BRSK2 BR serine/threonine kinase 2 XM_017018535.1 69.1% 21.9% (many diffs)
15 human 9024 BRSK2 BR serine/threonine kinase 2 XM_017018534.1 68.6% 21.7% (many diffs)
16 human 9024 BRSK2 BR serine/threonine kinase 2 XM_017018532.1 67.9% 21.3% (many diffs)
17 human 84446 BRSK1 BR serine/threonine kinase 1 NM_032430.2 65.2% 30.3% (many diffs)
18 human 84446 BRSK1 BR serine/threonine kinase 1 XM_024451739.1 60.2% 23.9% (many diffs)
19 human 9024 BRSK2 BR serine/threonine kinase 2 XR_930925.1 49.8% (many diffs)
20 human 9024 BRSK2 BR serine/threonine kinase 2 XR_242831.2 49.3% (many diffs)
21 human 9024 BRSK2 BR serine/threonine kinase 2 XR_002957207.1 49.2% (many diffs)
22 human 9024 BRSK2 BR serine/threonine kinase 2 NR_046331.2 46.8% (many diffs)
23 human 9024 BRSK2 BR serine/threonine kinase 2 XR_001748025.1 45.5% (many diffs)
24 human 9024 BRSK2 BR serine/threonine kinase 2 XR_930926.2 44.5% (many diffs)
25 human 9024 BRSK2 BR serine/threonine kinase 2 XR_001748026.2 41.1% (many diffs)
26 human 9024 BRSK2 BR serine/threonine kinase 2 XM_017018538.1 40.4% 2.8% (many diffs)
27 mouse 75770 Brsk2 BR serine/threonine kinase 2 NM_029426.2 83.1% 37.6% (many diffs)
28 mouse 75770 Brsk2 BR serine/threonine kinase 2 NM_001009929.3 82.7% 36.4% (many diffs)
29 mouse 75770 Brsk2 BR serine/threonine kinase 2 NM_001276763.1 81.7% 36.4% (many diffs)
30 mouse 75770 Brsk2 BR serine/threonine kinase 2 NM_001009930.3 78.8% 34.2% (many diffs)
31 mouse 381979 Brsk1 BR serine/threonine kinase 1 NM_001168572.1 37.1% 68.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
816
ORF length:
747
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gacatcgacg gggaaggacg gcggcgcgca gcacgcgcag tatgttgggc
121cctaccggct ggagaagacg ctgggcaagg ggcagacagg tctggtgaag ctgggggttc
181actgcgtcac ctgccagaag gtggccatca agatcgtcaa ccgtgagaag ctcagcgagt
241cggtgctgat gaaggtggag cgggagatcg cgatcctgaa gctcattgag cacccccacg
301tcctaaagct gcacgacgtt tatgaaaaca aaaaatattt gtacctggtg ctagaacacg
361tgtcaggtgg tgagctcttc gactacctgg tgaagaaggg gaggctgacg cctaaggagg
421ctcggaagtt cttccggcag atcatctctg cgctggactt ctgccacagc cactccatat
481gccacaggga tctgaaacct gaaaacctcc tgctggacga gaagaacaac atccgcatcg
541cagactttgg catggcgtcc ctgcaggttg gcgacagcct gttggagacc agctgtgggt
601ccccccacta cgcctgcccc gaggtgatcc ggggggagaa gtatgacggc cggaaggcgg
661acgtgtggag ctgcggcgtc atcctgttcg ccttgctggt gggggctctg cccttcgacg
721atgacaactt gcgacagctg ctggagaagg tgaagcgggg cgtgttccac atgccgcact
781ttatcccgcc cgactgccag agtctgctac gggcatgatc gaggtggacg cggcacgcgc
841tcacgctaga gcacattcag accacatatg gtatataggg ggcaagaatg agcccgaacc
901agagcagccc attcctcgca aggtgcagat ccgctcgctg cccagcctgg aggacatcga
961ccccgacgtg ctggacagca tgcactcact gggctgcttc cgagaccgca acaagctgct
1021gcaggacctg ctgtccgagg aggagaacca ggagaagatg atttacttcc tcctcctgga
1081ccggaaagaa aggtacccga gccaggagga tgaggacctg cccccccgga acgagataga
1141ccctccccgg aagcgtgtgg actccccgat gctgaaccgg cacggcaagc ggcggccaga
1201acgcaaatcc atggaggtgc tcagcgtgac ggacggcggc tccccggtgc ttgcgcgggg
1261cccatccccc aatttacgca aggaagaaag tttttgggtt ccttaaaggg ggcttccttg
1321aggcttttcc caccagcccc atccaggagg cccccggggg gcccccttaa ccccttccac
1381caagggccat tcccccctcc cccccccccc ccagggacac ctgtccacac gccaaaggag
1441agcccggctg gcacgcccaa cccccaaggc ccccgtccag ccccagcgtc ggaggggtgc
1501cctggagggc gcggctcaac tccatcaaga acagctttct gggctcaccc cgcttccacc
1561gccggaaact gcaagttccg acgccggagg agatgtccaa cctgacacca gagtcgtccc
1621cagagctggc gaagaagtcc tggtttggga acttcatcag cctggagaag gaggagcaga
1681tcttcgtggt catcaaagac aaacctctga gctccatcaa ggctgacatc gtgcacgcct
1741tcctgtcgat tcccagtctc agccacagcg tcatctccca aacgagcttc cgggccgagt
1801acaaggccac gggggggcca gccgtgttcc agaagccggt caagttccag gttgatatca
1861cctacacgga gggtggggag gcgcagaagg agaacggcat ctactccgtc accttcaccc
1921tgctctcagg ccccagccgt cgcttcaaga gggtggtgga gaccatccag gcccagctgc
1981tgagcacaca cgacccgcct gcggcccagc acttgtcaga caccactaac tgtatggaaa
2041tgatgacggg gcggctttcc aaatgtggaa ttatcccgaa aagtttgcca actttcttgt
2101acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
2161gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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