Construct: ORF ccsbBroad304_14944

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018910.1_s304c1, BRDN0000459394
Derived from:
ccsbBroadEn_14944
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
ULK2 (9706)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14944
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9706 ULK2 unc-51 like autophagy activ... NM_001142610.2 99.3% 73.9% (many diffs)
2 human 9706 ULK2 unc-51 like autophagy activ... NM_014683.4 99.3% 73.9% (many diffs)
3 human 9706 ULK2 unc-51 like autophagy activ... XM_017025424.2 97.3% 72.4% (many diffs)
4 human 9706 ULK2 unc-51 like autophagy activ... XM_017025425.2 97.3% 72.4% (many diffs)
5 human 9706 ULK2 unc-51 like autophagy activ... XM_017025426.2 94.8% 69.9% (many diffs)
6 human 9706 ULK2 unc-51 like autophagy activ... XM_011524087.2 87.9% 62.5% (many diffs)
7 human 9706 ULK2 unc-51 like autophagy activ... XM_017025427.2 74.4% 50% (many diffs)
8 human 9706 ULK2 unc-51 like autophagy activ... XR_001752701.2 73.9% (many diffs)
9 human 9706 ULK2 unc-51 like autophagy activ... XM_017025428.2 71.8% 47.3% (many diffs)
10 human 9706 ULK2 unc-51 like autophagy activ... XR_001752700.2 49.8% (many diffs)
11 mouse 29869 Ulk2 unc-51 like kinase 2 NM_013881.4 89.9% 68.7% (many diffs)
12 mouse 29869 Ulk2 unc-51 like kinase 2 XM_006533508.3 88.4% 67.8% (many diffs)
13 mouse 29869 Ulk2 unc-51 like kinase 2 XM_006533509.3 84.4% 63.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2478
ORF length:
2409
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaggtggtg ggtgacttcg agtacagcaa gagggatctc gtgggacacg
121gggccttcgc cgtggtcttc cgggggcggc accgccagaa aactgattgg gaggtagcta
181ttaaaagtat taataaaaag aacttgtcaa aatcacaaat actgcttgga aaggaaatta
241aaatcttaaa ggaacttcag catgaaaata ttgtagcact ctatgatgtt caggaattac
301ccaactctgt ctttttggtg atggagtatt gcaatggtgg agacctcgca gattatttgc
361aagcgaaagg gactctcagt gaagacacga tcagagtgtt tctgcatcag attgctgctg
421ccatgcgaat cctgcacagc aaaggaatca tccacagaga tctcaaacca cagaacatct
481tgctgtccta tgccaatcgc agaaaatcaa gtgtcagtgg tattcgcatc aaaatagcgg
541attttggttt tgctcgttac ctacatagta acatgatggc tgcaacactg tgtggatccc
601cgatgtacat ggctcctgag gttattatgt ctcaacatta tgatgctaag gctgacttgt
661ggagcatagg aacagtgata taccaatgcc tagttggaaa accacctttt caggccaata
721gtcctcaaga cttaaagatg ttttatgaaa aaaacaggag cttaatgcct agtattccca
781gagaaacatc accttatttg gctaatctct tttgggtttg cttcagagaa accaaaaaga
841taagaatgga ctttgaagcg tttttagcca tcctttttct tgagcaaggt ccagtaaaaa
901aatcttgccc agttccagtg cccatgtatt ctggttctgt ctctggaagc tcctgtggca
961gctctccatc ttgtcgtttt gcttctccac catcccttcc agatatgcag catattcagg
1021aagaaaactt atcttcccca ccattgggtc ctcccaacta tctacaagtt tccaaagatt
1081ctgccagtac tagtagcaag aactcttctt gtgacacgga tgactttgtt ttggtgccac
1141acaacatctc gtcagaccac tcatgtgata tgccaatggg gactgctggc agacgtgctt
1201caaatgaatt cttggtgtgt ggagggcagt gtcagcctac tgtgtcacct cacagcgaaa
1261cagcaccaat tccagttcct actcaaataa ggaattatca gcgcatagag cagaatctta
1321catctactgc cagctcaggc acaaatgtac atggttctcc aagatctgca gtggtacgaa
1381ggtccaacac cagccccatg ggcttcctcc ggccgggatc atgctcccca gtaccagcag
1441acacagcaca gacagttgga cgaaggctct ccactgggtc ttctaggcct tactcacctt
1501cccctttggt tggtaccatt cctgagcaat tcagtcagtg ctgctgtggg catcctcagg
1561gccatgactc caggagtaga aactcctcag gttctccagt gccacaagct cagtccccac
1621agtctctctt atcgggtgct agactgcaga gcgcccccac cctcactgac atctatcaga
1681acaagcagaa gctcagaaaa cagcactctg accccgtgtg cccatcccat actggggctg
1741ggtacagcta ctcgcctcag cccagtcggc ctggcagcct tggaacttct cccaccaagc
1801acttggggtc ctctccacgg agttctgact ggttctttaa aactcctttg ccaacaatca
1861ttggctctcc tactaagacc acagctcctt tcaaaatccc taaaactcaa gcatcttcca
1921acctgttagc cttggttact cgtcatgggc ctgctgaaga acagtcgaaa gatgggaatg
1981agccacggga atgtgcccat tgcctcttag tgcaaggaag tgagaggcag cgggccgagc
2041agcagagcaa ggcagtgttt ggcagatctg tcagtaccgg gaagttatca gatcaacaag
2101gaaagactcc tatatgtcga catcagggca gcacagacag tttaaataca gaacgaccaa
2161tggatatagc tccggcagga gcctgtggtg gtgttctggc acctcctgca ggtacagcag
2221caagttccaa ggctgtcctc ttcactgtag ggtctcctcc acacagtgcg gcagccccca
2281cttgtaccca catgttcctt cgaacaagaa caacctcagt ggggcccagc aactccgggg
2341gctctctttg tgccatgagt ggccgcgttt gcgtggggtc ccccgcttgc cccaagcctt
2401cgggtctttc cctttccagg agcagaggca gctcccagcc tgagatacgt gccttacggt
2461gcttcacccc cccagcctag agggctcatc acctttgaag cccctgaact gccggaggag
2521acgctgatgg agcgggaaca cacagacacc ttacgccatc tgaatgtgat gctgatgttc
2581actgagtgtg tgctggacct gacagccatg aggggaggaa accntgagct gtgcacatct
2641gctgtgtcct tgtaccagat ccaggagagt gtggtggtgg accagatcag tcagctgagc
2701aaagactggg ggcgggtgga gcagctggtg ttgtacatga aagcagcaca gctgcttgcg
2761gcttctctgc atcttgccaa agcccagatc aagtccggga aactgagccc atccacagct
2821gtgaaacaag ttgtcaagaa tctgaacgaa cgatataaat tctgcatcac catgtgcaag
2881aaacttacag aaaagctgaa tcgattcttc tctgacaaac agaggtttat tgatgaaatc
2941aacagtgtga ctgcagagaa actcatctat aattgtgctg tagaaatggt tcagtctgca
3001gccctggatg agatgtttca gcagaccgaa gatattgttt atcgctatca taaggcagcc
3061cttcttttgg aaggcctaag taggattcta caggaccctg cagatattga aaatgtgcat
3121aaatataaat gtagtattga gagaagactg tcggcgctct gccatagcac cgcaaccgtg
3181ttgccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc
3241gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc
3301tggatta

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